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The predictive value of basal follicle stimulating and growth hormone levels as determined by immunofluorometry during assisted reproduction

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Abstract

Purpose

Our purpose was (a) to investigate relationships of baseline endocrine serum levels with selected assisted reproduction (AR) parameters and (b) to evaluate the clinical applicability of an immunofluorometric assay (IFMA) as an alternative to a radioimmunoassay (RIA).

Methods

Basal endocrine values (analyzed by RIA—rFSH, rLH, and rE2; analyzed by IFMA—fFSH, fLH, and fGH) were determined for female patients (n = 142) preceding ovarian stimulation for AR.

Results

Specific AR parameters correlated significantly with RIA- and IFMA-determined FSH levels, although IFMA correlations consistently exceeds that of RIA. Cutoff values of fFSH ⩾ 11.68 IU/L or rFSH ⩾ 15.0 IU/L indicated a poor response. The high-basal fFSH group was older (34 vs 31 years; P = 0.0334) and yielded fewer oocytes (2.9 vs 4.6 oocytes; P = 0.0018) than the low-basal fFSH group (<11.68 IU/L). Lower cumulative embryo scores and conception rates were also associated with the high-fFSH group, compared to the low-fFSH group.

Conclusions

This study confirms the negative impact of elevated basal FSH levels on AR. Basal LH, GH, and E2 levels are, in comparison to baseline FSH levels, unsuitable indices for estimating ovarian responsiveness. IFMA can be regarded as an alternative to RIA in both clinical and research laboratories.

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Authors and Affiliations

  1. Centre for Fertility Studies, Department of Obstetrics and Gynaecology, University of Pretoria, P.O. Box 667, 0002, Pretoria, RSA

    Carin Huyser, Francois Le R. Fourie & Johan Pentz

  2. Department of Chemical Pathology, University of Pretoria, Pretoria, RSA

    Philip Hurter

Authors
  1. Carin Huyser
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  2. Francois Le R. Fourie
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  3. Johan Pentz
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  4. Philip Hurter
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Huyser, C., Fourie, F.L.R., Pentz, J. et al. The predictive value of basal follicle stimulating and growth hormone levels as determined by immunofluorometry during assisted reproduction. J Assist Reprod Genet 12, 244–251 (1995). https://doi.org/10.1007/BF02212926

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  • DOI: https://doi.org/10.1007/BF02212926

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