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Mechanisms of biliary excretion of lithocholate-3-sulfate in Eisai hyperbilirubinemic rats (EHBR)

Digestive Diseases and Sciences volume 40, pages 1792–1797 (1995)Cite this article

Abstract

Biliary excretion of lithocholate-3-sulfate is markedly impaired in EHBR. To examine the mechanism of biliary lithocholate-3-sulfate excretion in EHBR, the effects of colchicine treatment, a vesicular transport inhibitor, and infusion of taurocholate and organic anions were studied in EHBR and Sprague-Dawley rats. Colchicine treatment and taurocholate infusion had no effect on biliary lithocholate-3-sulfate excretion in EHBR, suggesting that biliary lithocholate-3-sulfate excretion is not mediated by the vesicular transport or by the bile acid excretory pathway. In control Sprague-Dawley rats, both sulfobromophthalein and dibromosulfophthalein infusion inhibited biliary lithocholate-3-sulfate excretion. In contrast, in EHBR dibromosulfophthalein infusion inhibited biliary lithocholate-3-sulfate excretion but BSP infusion did not. Indocyanine green and pravastatin infusion did not affect biliary lithocholate-3-sulfate excretion but pravastatin infusion had no effect in EHBR. These findings indicate that, whether physiologically important or not, two or more excretory pathways for organic anions exist at the canalicular membrane other than the ATP-dependent one.

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Affiliations

  1. Department of Medicine, Teikyo University School of Medicine, Kaga 2-11-1, Itabashi-ku, 173, Tokyo, Japan

    Hajime Takikawa MD, Kou Nishikawa MD, Naoyo Sano PhD, Masami Yamanaka MD & Tohru Horie PhD

  2. Tsukuba Research Laboratories, Eisai Co., Ltd., Ibaraki, Japan

    Hajime Takikawa MD, Kou Nishikawa MD, Naoyo Sano PhD, Masami Yamanaka MD & Tohru Horie PhD

Authors
  1. Hajime Takikawa MD
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  2. Kou Nishikawa MD
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  3. Naoyo Sano PhD
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  4. Masami Yamanaka MD
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  5. Tohru Horie PhD
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This study was funded in part by a Grant-in-Aid for General Scientific Research (C) 06670583 from the Japanese Ministry of Education, Science and Culture.

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Takikawa, H., Nishikawa, K., Sano, N. et al. Mechanisms of biliary excretion of lithocholate-3-sulfate in Eisai hyperbilirubinemic rats (EHBR). Digest Dis Sci 40, 1792–1797 (1995). https://doi.org/10.1007/BF02212704

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  • DOI: https://doi.org/10.1007/BF02212704

Key Words

  • bile acid
  • organic anion
  • biliary excretion
  • hepatic transport