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Role of prostaglandins and histamine in hyperemic response to superficial and deep gastric mucosal injury and H+ back-diffusion in cats

  • Esophageal, Gastric, and Duodenal Disorders
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Abstract

This study was undertaken to examine the role of prostaglandins and histamine in the hyperemic response to gastric mucosal damage followed by H+ back-diffusion. Cat stomachs were exposed to 2 mol/liter NaCl for 10 min followed by luminal perfusion at pH 1. Hypertonic saline caused extensive (microscopic) damage to the surface epithelium, increased gastric mucosal blood flow, and increased release of histamine, PGE2, and 6-keto PGF1α (prostacyclin) into portal venous blood. The effect of indomethacin and histamine blockers (H1+H2) on the hyperemic response to acid back-diffusion was related to the depth of the mucosal injury and the region of the stomach. In the corpus, indomethacin enhanced mucosal injury. In areas with superficial damage, the hyperemia was inhibited by indomethacin and antihistamines and eliminated by the combination of both. In corpus areas with indomethacin-induced deep lesions, the blood flow was very high, and this hyperemia was partly inhibited by antihistamines. In the antrum the hyperemic response was reduced by antihistamines. Indomethacin increased the release of histamine into the portal venous blood (baseline recordings) and reduced basal gastric mucosal blood flow.

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References

  1. Svanes K, Gislason H, Guttu K, Herfjord JK, Fevang J, Grønbech JE: Role of blood flow in adaptive protection of the cat gastric mucosa. Gastroenterology 100:1249–1258, 1991

    Google Scholar 

  2. Livingston EH, Guth PH: Antisecretory and cytoprotective doses of enprostil do not alter gastric mucosal blood flow. Life Sci 52:1621–1628, 1993

    Google Scholar 

  3. Leung FW, Robert A, Guth PH: Gastric mucosal blood flow in rats after administration of 16,16-dimethyl prostaglandin E2 at a cytoprotective dose. Gastroenterology 88:1948–1953, 1985

    Google Scholar 

  4. Whittle BJR, Vane JR: Prostanoids as regulators of gastrointestinal function.In LR Johnson (ed). Physiology of the Gastrointestinal Tract, 2nd ed. New York, Raven Press, 1987, pp 143–180

    Google Scholar 

  5. Holzer P, Guth PH: Neuropeptide control of rat gastric mucosal blood flow. Increase by calcitonin generalated peptide and vasoactive intestinal polypeptide, but not substance P and neurokinin A. Circ Res 68:100–105, 1991

    Google Scholar 

  6. Li DS, Raybould HE, Quintero E, Guth PH: Calcitonin generelated peptide mediates the gastric hyperemic response to acid back-diffusion. Gastroenterology 102:1124–1128, 1992

    Google Scholar 

  7. Whittle BJR, Lopez-Belmonte J, Moncada S: Regulation of gastric mucosal integrity by endogenous nitric oxide: Interactions with prostanoids and sensory neuropeptides in the rat. Br J Pharmacol 99:607–611, 1990

    Google Scholar 

  8. Wood JG, Darnell MP, Cheung LY: Adenosine is a mediator of ethanol-induced gastric vasodilation in dogs. Am J Physiol 264:G664-G670, 1993

    Google Scholar 

  9. Gislason H, Guttu K, Sørbye H, Schifter S, Waldum HL, Svanes K: Role of histamine and calcitonin gene-related peptide (CGRP) in the hyperemic response to hypertonic saline and H+ back-diffusion in the gastric mucosa of cats. Scand J Gastroenterol 30:300–310, 1994

    Google Scholar 

  10. Endoh K, Kao J, Domek MJ, Leung FW: Mechanism of gastric hyperemia induced by intragastric hypertonic saline in rats. Gastroenterology 104:114–121, 1993

    Google Scholar 

  11. Ritchie WP, Mercer D: Mediators of bile acid-induced alterations in gastric mucosal blood flow. Am J Surg 161:126–130, 1991

    Google Scholar 

  12. Gerkens JF, Flexner C, Oates JA, Shand DG: Prostaglandin and histamine involvement in the gastric vasodilator action of pentagastrin. J Pharmacol Exp Ther 201:421–426, 1977

    Google Scholar 

  13. Hogaboam CM, Bissonnette EY, Chin BC, Befus AD, Wallace JL: Prostaglandins inhibit inflammatory mediator release from rat mast cells. Gastroenterology 104:122–129, 1993

    Google Scholar 

  14. Sandvik AK, Waldum HL: The effect of misoprostol on baseline and stimulated acid secretion and on gastrin and histamine release in the totally isolated, vascularly perfused rat stomach. Scand J Gastroenterol 23:696–700, 1988

    Google Scholar 

  15. Matuoka K, Masanobu T, Mitsui Y, Murota S: Cultured rabbit gastric epithelial cells producing prostaglandin I2. Gastroenterology 84:498–505, 1983

    Google Scholar 

  16. Levine RA, Kohen KR, Schwartzel EH, Ramsey CE: Prostaglandins E2 histamine interactions on cAMP, cGMP and acid production in isolated fundic glands. Am J Physiol 242:G21-G26, 1982

    Google Scholar 

  17. Heyman MA, Payne BD, Hoffman JIE, Rudolph AM: Blood flow measurements with radionuclide-labelled particles. Prog Cardiovasc Dis 20:619–625, 1977

    Google Scholar 

  18. Waldum HL, Sandvik AK, Brenna E, Søgnen BS: Radioimmunoassay of histamine. Scand J Gastroenterol 26(suppl 180):32–39, 1991

    Google Scholar 

  19. Farman N, Pradelles P, Bonvalet JP: PGE2, PGF2α, 6-keto-PGF1α, and TxB2 synthesis along the rabbit nephron. Am J Physiol 252:F53-F59, 1987

    Google Scholar 

  20. Maxey KM, Maddipati KR, Birkmeier J: Interference in enzyme immunoassays. J Clin Immunoassay 15:116–120, 1992

    Google Scholar 

  21. Kelly RW, Graham BJM, O'Sullivan MJ: Measurement of PGE2 as the methyl oxime by radioimmunoassay using a novel lodinated label. Prostaglandins Leukotrienes Essent Fatty Acids 37:187–191, 1989

    Google Scholar 

  22. Lacy ER, Ito S: Microscopic analysis of ethanol damage to rat gastric mucosa after treatment with a prostaglandin. Gastroenterology 83:619–625, 1982

    Google Scholar 

  23. Sørbye H, Guttu K, Gislason H, Grong K, Svanes K: Gastric mucosal injury and associated changes in mucosal blood flow and gastric fluid secretion caused by dimethyl sulfoxide (DMSO) in rats. Dig Dis Sci 38:1243–1250, 1993

    Google Scholar 

  24. Richards DM, Brogden RN, Heel RC, Speight TM, Avery GS: Astemizole. A review of its pharmacodynamic properties and therapeutic efficacy. Drugs 28:38–61, 1984

    Google Scholar 

  25. Brittain RT, Jack D, Reeves JJ, Stables R: Pharmacological basis for the induction of gastric carcinoid tumours in the rat by loxtidine, an unsurmountable histamine H2-receptor blocking drug. Br J Pharmacol 85:843–847, 1985

    Google Scholar 

  26. Grønbech JE, Matre K, Stangeland L, Svanes K, Varhaug JE: Gastric mucosal repair in the cat: Role of the hyperemic response to mucosal damage. Gastroenterology 95:311–320, 1988

    Google Scholar 

  27. Waldum HL, Sandvik AK, Brenna E, Petersen H: Gastrin-histamine sequence in the regulation of gastric acid secretion. Gut 32:32–39, 1991

    Google Scholar 

  28. Andersson K, Mattsson H, Larsson H: The role of gastric mucosal histamine in acid secretion and experimentally induced lesions in the rat. Digestion 46:1–9, 1990

    Google Scholar 

  29. Konturek SJ, Brzozowski T, Piastucki I, Radecki T, Dembinski A, Dembinska-Kiec A: Role of locally generated prostaglandins in adaptive gastric cytoprotection. Dig Dis Sci 27:967–971, 1982

    Google Scholar 

  30. Kauffman GL, Whittle BJR, Salmon JA: Gastric venous prostaglandin concentrations during basal and stimulated acid secretion in dog. Proc Soc Exp Med 169:233–238, 1982

    Google Scholar 

  31. Main IHM, Whittle BRJ: Investigation of the vasodilator and antisecretory role of prostaglandins in the rat gastric mucosa by use of non-steroidal anti-inflammatory drugs. Br J Pharmacol 53:217–224, 1975

    Google Scholar 

  32. Guth PH, Moler TL: The role of endogenous prostanoids in the response of the rat gastric microcirculation to vasoactive agents. Microvasc Res 23:336–346, 1982

    Google Scholar 

  33. Skarstein A: Effect of indomethacin on blood flow distribution in the stomach of cats with acute gastric ulcer. Scand J Gastroenterol 14:905–911, 1979

    Google Scholar 

  34. Guth PH, Paulsen G, Nagata H: Histologic and microcirculatory changes in alcohol-induced gastric lesions in the rat. Effect of prostaglandin cytoprotection. Gastroenterology 87:1083–1090, 1984

    Google Scholar 

  35. Morris GP, Wallace JL: The roles of ethanol and of acid in the production of gastric mucosal erosions in rats. Virchows Arch (Cell Pathol) 38:23–38, 1981

    Google Scholar 

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Authors and Affiliations

  1. Surgical Research Laboratory, and Department of Clinical Biology, Division of Endocrinology, University of Bergen, Norway

    Hjörtur Gislason MD, Halfdan Sørbye MD, Farzad Abdi-Dezfuli MD, Helge L. Waldum MD & Knut Svanes MD

  2. University of Trondheim, Norway

    Hjörtur Gislason MD, Halfdan Sørbye MD, Farzad Abdi-Dezfuli MD, Helge L. Waldum MD & Knut Svanes MD

Authors
  1. Hjörtur Gislason MD
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  2. Halfdan Sørbye MD
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  3. Farzad Abdi-Dezfuli MD
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  4. Helge L. Waldum MD
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  5. Knut Svanes MD
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Gislason, H., Sørbye, H., Abdi-Dezfuli, F. et al. Role of prostaglandins and histamine in hyperemic response to superficial and deep gastric mucosal injury and H+ back-diffusion in cats. Digest Dis Sci 40, 1669–1678 (1995). https://doi.org/10.1007/BF02212687

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  • DOI: https://doi.org/10.1007/BF02212687

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