Journal of Assisted Reproduction and Genetics

, Volume 11, Issue 8, pp 428–432 | Cite as

Improvements in assessment of human failed fertilization oocytes using the supravital dye hoechst 33342

  • E. Neuber
  • J. Witmyer
  • S. P. Oskowitz
  • R. D. Powers
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  • 23 Downloads

Keywords

Public Health Developmental Biology Failed Fertilization Fertilization Oocyte 

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References

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    Arndt-Jovin D, Jovin T: Analysis and sorting of living cells according to deoxyribonucleic acid content. J Histochem Cytochem 1977;25:585–589PubMedGoogle Scholar
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    Luttmer S, Longo F: Examination of living and fixed gametes and early embryos stained with supra vital fluorochromes (Hoechst 33342 and 3,3′-dihexyloxacarbocyanine Iodine). Gamete Res 1986;15:267–283Google Scholar
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    Latt SA, Stetten G: Spectral studies on 33258 Hoechst and related bisbenzimidazole dyes useful for fluorescent detection of deoxyribonucleic acid synthesis. J Histochem Cytochem 1976;24:24–33PubMedGoogle Scholar
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    Gwatkin RBL, Conover JC, Collins RL, Quigley MM: Failed fertilization in humanin vitro fertilization analyzed with the deoxyribonucleic acid-specific fluorochrome Hoechst 33342. Am J Obstet Gynecol 1989;160:31–35PubMedGoogle Scholar
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    Schalkoff ME, Oskowitz SP, Powers RD: A multifactorial analysis of the pregnancy outcome in a successful embryo cryopreservation program. Fertil Steril 1993;59:1070–1074PubMedGoogle Scholar
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    Van Wissen B, Bomsel-Helmreich O, Debey P, Eisenberg C, Vautier D, Pennehouat G: Fertilization and ageing processes in non-dividing human oocytes after GnRHa treatment: An analysis of individual oocytes. Human Reprod 1991;6:879–884Google Scholar
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    Pieters M, Dumoulin J, Englehart C, Bras M, Evers J, Geraedts J: Immaturity and aneuploidy in human oocytes after different stimulation protocols. Fertil Steril 1991;56:306–310PubMedGoogle Scholar

Copyright information

© Plenum Publishing Corporation 1994

Authors and Affiliations

  • E. Neuber
    • 2
  • J. Witmyer
    • 2
  • S. P. Oskowitz
    • 2
  • R. D. Powers
    • 1
    • 2
  1. 1.Department of BiologyBoston CollegeChestnut Hill
  2. 2.Boston IVFBrookline

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