Journal of Assisted Reproduction and Genetics

, Volume 11, Issue 8, pp 428–432 | Cite as

Improvements in assessment of human failed fertilization oocytes using the supravital dye hoechst 33342

  • E. Neuber
  • J. Witmyer
  • S. P. Oskowitz
  • R. D. Powers
Short Communications


Public Health Developmental Biology Failed Fertilization Fertilization Oocyte 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Arndt-Jovin D, Jovin T: Analysis and sorting of living cells according to deoxyribonucleic acid content. J Histochem Cytochem 1977;25:585–589PubMedGoogle Scholar
  2. 2.
    Luttmer S, Longo F: Examination of living and fixed gametes and early embryos stained with supra vital fluorochromes (Hoechst 33342 and 3,3′-dihexyloxacarbocyanine Iodine). Gamete Res 1986;15:267–283Google Scholar
  3. 3.
    Latt SA, Stetten G: Spectral studies on 33258 Hoechst and related bisbenzimidazole dyes useful for fluorescent detection of deoxyribonucleic acid synthesis. J Histochem Cytochem 1976;24:24–33PubMedGoogle Scholar
  4. 4.
    Gwatkin RBL, Conover JC, Collins RL, Quigley MM: Failed fertilization in humanin vitro fertilization analyzed with the deoxyribonucleic acid-specific fluorochrome Hoechst 33342. Am J Obstet Gynecol 1989;160:31–35PubMedGoogle Scholar
  5. 5.
    O'Rand MG, Herman B, Diguiseppi J, Halme J, Hammond MG, Talbert LM: Analysis of deoxyribonucleic acid distribution in non cleaving oocytes from patients undergoingin vitro fertilization. Fertil Steril 1986;46:452–460PubMedGoogle Scholar
  6. 6.
    Schalkoff ME, Oskowitz SP, Powers RD: A multifactorial analysis of the pregnancy outcome in a successful embryo cryopreservation program. Fertil Steril 1993;59:1070–1074PubMedGoogle Scholar
  7. 7.
    Van Wissen B, Bomsel-Helmreich O, Debey P, Eisenberg C, Vautier D, Pennehouat G: Fertilization and ageing processes in non-dividing human oocytes after GnRHa treatment: An analysis of individual oocytes. Human Reprod 1991;6:879–884Google Scholar
  8. 8.
    Pieters M, Dumoulin J, Englehart C, Bras M, Evers J, Geraedts J: Immaturity and aneuploidy in human oocytes after different stimulation protocols. Fertil Steril 1991;56:306–310PubMedGoogle Scholar

Copyright information

© Plenum Publishing Corporation 1994

Authors and Affiliations

  • E. Neuber
    • 2
  • J. Witmyer
    • 2
  • S. P. Oskowitz
    • 2
  • R. D. Powers
    • 1
    • 2
  1. 1.Department of BiologyBoston CollegeChestnut Hill
  2. 2.Boston IVFBrookline

Personalised recommendations