Cytogenetic analysis of human oocytes parthenogenetically activated by puromycin
Treatment of aged human oocytes by puromycin allows a high rate of parthenogenetic activation and development until the first cleavage division. This technique was used for the study of the chromosome complement of oocytes which remained unfertilized after in vitro fertilization. Three hundred four unfertilized oocytes were treated with 10 Μg/ml puromycin for 6–8 hr and further cultured for 12–15 hr.
Activation occurred in 90.5% of the oocytes. Heterozygous diploids with two pronuclei predominated (61%), which is in contrast to the mouse, where the majority of oocytes activated by puromycin are uniform haploids (89%).
Therefore we conclude that puromycin treatment induces retention of the second polar body in human oocytes, unlike in mouse oocytes treated in the same way. Chromosome analysis performed on 182 oocytes suggested a nondisjunction (ND) rate for the second meiotic division of 12.7%. This is a low figure considering the fact that puromycin itself has been reported to induce nondisjunction. For the first meiotic division a ND rate of only 5.6% was found. This rate is lower than the one found in metaphase II arrested oocytes and we believe that this difference is due to the technical differences between the study of meiotic and that of mitotic chromosomes.
Key wordschromosomes human oocytes parthenogenesis puromycin
Unable to display preview. Download preview PDF.
- 9.De Sutter P: Non-random chromosome nondisjunction at the first meiotic division induced by puromycin in the mouse.In abstract book of the IXth Meeting of the Contact Group for IVF, National Scientific Research Foundation, Brussels, 1993, p 15Google Scholar
- 14.Kaufman MH: The ovulation and activation of primary and secondary oocytes in Lt/Sv strain mice. Gamete Res 1986; 14:255–264Google Scholar
- 17.Balakier H, Casper RF: A morphologic study of unfertilized oocytes and abnormal embryos in human in vitro fertilization. J Vitro Fert Embryo Transfer 1991;8:73–79Google Scholar
- 19.Procházka R, Kaňka J, šutovský P, Fulka J, Motlík J: Development of pronuclei in pig oocytes activated by a single electric pulse. J Reprod Fert 1992;96:725–734Google Scholar