Plant and Soil

, Volume 57, Issue 2–3, pp 399–405 | Cite as

Enumeration of acetylene-reducing bacteria in strip-mined reclamation sites in a temperate grassland

  • M. A. Koponen
  • B. R. Funke
  • D. S. Galitz


From 36 to 71% of bacteria, depending on the sampling site, that were isolated from the soil or rhizosphere of undisturbed prairie soil or reclamation sites of strip-mined grassland areas in western North Dakota were capable of reducing acetylene. These bacteria generally could be divided into two populations; one capable of acetylene reduction under aerobic conditions and another capable of acetylene reduction under anaerobic conditions. The reclamation site to which no topsoil had been applied, pH 8.5, had a bacterial population which generally was capable of higher levels of acetylene reduction than individual bacteria isolated from other sites.

Key Words

Acetylene reduction Asymbiotic nitrogen fixation Grasslands Rhizosphere Stripmined land reclamation 


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Copyright information

© Martinus Nijhoff Publishers 1980

Authors and Affiliations

  • M. A. Koponen
    • 1
  • B. R. Funke
    • 1
  • D. S. Galitz
    • 2
  1. 1.Dept. of BacteriologyNorth Dakota State UniversityFargo
  2. 2.Dept. of BotanyNorth Dakota State UniversityFargo

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