Abstract
Purpose
The effects of dam strain on the spontaneous incidence of the cleft lip and palate (CLP) and the intrauterine growth of transferred CLP-susceptible CL/Fr embryos were examined with an embryo transfer technique. The CL/Fr strain of embryos at the early blastocyst stage was transferred to the same dam strain and also to the CLP-resistant C57BL dam strain. A laparotomy was done on the 18th gestational day at which time the number of fetuses, the resorption sites and the fetus weight were recorded. Each fetus was checked for the presence of CLP. Five criteria to assess the reproduction and fertility as well as the fetus weight were then compared between both dam strains.
Results
The dam pregnancy rate and the fetus survival rate in the CL/Fr dam strain were both significantly lower than those in the C57BL dam strain. The resorption rate in the CL/Fr dam strain was significantly higher than that in the C57BL dam strain. The spontaneous incidence rate of CLP in the CL/Fr dam strain was also significantly higher than that in the C57BL dam strain. The fetus weight of the CL/Fr fetuses developed in the CL/Fr dam strain was significantly lighter than that of the CL/Fr fetuses developed in the C57BL dam strain.
Conclusion
The results indicated that the CLP-susceptible CL/Fr dam strain provided a less favorable uterine environment for the implantation, survival and intrauterine growth of the transferred CL/Fr embryos and also caused a higher spontaneous incidence rate of CLP. Thus, it can be concluded that the effect of the dam strain appears to play an important role on the spontaneous incidence of CLP and the intrauterine growth of the CL/ Fr strain embryos transferred to both CL/Fr and C57BL dam strains.
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References
Carter CO: The inheritance of common congenital malformations. Prog Med Genet 1965;5:59–84
Bhatia SN: Genetics of cleft lip and palate. Br Dent J 1972;132:95–103
Millicovsky G, Johnston MC, Ambrose LJH: Developmental alterations associated with spontaneous cleft lip and palate in CL/Fr mice. J Am Anat 1982;164:29–44
Gasser DL, Mele L, Lees DD, Goldman AS: Genes in mice that affect susceptibility to cortisone-induced cleft palate are closely linked to Ir genes on chromosomes 2 and 17. Proc Natl Acad Sci USA 1981;78:3147–3150
Esaki K: Decrease in cleft lip incidence by urethane administration to CL/Fr mice. Cong Anom 1983;23:395–398
Liu SL, Erickson RP: Genetic differences among the A/J x C57BL/6J recombinant inbred mouse lines and their degree of association with glucocorticoid-induced cleft palate. Genetics 1986;113:745–754
Melnick M, Marazita ML, Hu DN: Genetic analysis of cleft lip with or without cleft palate in Chinese kindreds. Am J Med Genet Suppl 1987;2:183–190
Karolyi J, Liu S, Erickson RP: Genetics of susceptibility to 6-aminonicotinamide-induced cleft palate in the mouse: studies in congenic and recombinant inbred strains. Teratology 1988;37:283–287
Khoury MJ, Erickson JD, James LM: Maternal factors in cleft lip with or without cleft palate: Evidence from interracial crosses in the United States. Teratology 1983;27:351–357
Nomura T, Hata S, Kusafuka T: Suppression of developmental anomalies by maternal macrophages in mice. J Exp Med 1990;172:1325–1330
The Institute of Experimental Animals, Kyushu University: The annual report of the Institute of Experimental Animals, Kyushu University. Fukuoka, Kyushyu University, 1987
Martin DA, Nonaka K, Yanagita K, Nakata M: The effect of dam strain on the craniofacial morphogenesis of CL/Fr mouse fetuses. J Craniofac Genet Dev Biol 1995 (in press)
Whittingham JG: Culture of mouse ova. J Reprod Fert Suppl 1971;14:7–21
Biggers JD, Whitten WK, Whittingham DG: The culture of mouse embryos in vitro.In Methods in Mammalian Embryology, JC Daniel (ed). San Francisco, Freeman, 1971, pp 88–116
Bornstein S, Trasler DG, Fraser FC: Effect of uterine environment on frequency of spontaneous cleft lip in CL/Fr mice. Teratology 1970;2:371–376
Juriloff DM, Fraser FC: Genetic maternal effects on cleft lip frequency in A/J and CL/Fr mice. Teratology 1980;21:167–175
Kalter H: The inheritance of susceptibility to the teratogenic action of cortisone in mice. Genetics 1954;39:185–196
Kalter H: Modification of the teratogenic action of cortisone in mice by maternal age, maternal weight, and litter size. Am J Physiol 1956;185:65–68
Moler TL, Donahue SE, Anderson GB, Bradford GE: Effects of maternal and embryonic genotype on prenatal survival in two selected mouse lines. J Anim Sci 1981;51(2):300–303
McLaren A: Mechanisms affecting embryo development.In The Mammalian Oviduct, ESE Hafez, RJ Blandau (eds). Chicago, University of Chicago Press, 1968, pp 477–490
Kalter H: Sex, side, severity in spontaneous malformations of fetal A/JKt mice, and their associations with each other and with fetal weight. Teratology 1979;19:1–8
Kalter H: The history of the A family of inbred mice and biology of its congenital malformations. Teratology 1979;20: 213–232
Kalter H: Prenatal epidemiology of spontaneous cleft lip and palate, open eyelid, and embryonic death in A/J mice. Teratology 1975;12:245–257
Jacobson D, Trasler DG: Morphometric analysis of heterozygote dancer mice predisposed to 6-aminonicotinamideinduced cleft lip. Teratology 1992;45:393–400
Paria BC, Dey SK: Preimplantation embryo development in vitro: cooperative interactions among embryos and role of growth factors. Proc Natl Acad Sci USA 1990;87:4756–4760
Tamada H, McMaster MT, Flanders KC, Andrews GK, Dey SK: Cell type-specific expression of transforming growth factor-β1 in the mouse uterus during the periimplantation period. Mol Endoc 1990;4:965–972
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Nonaka, K., Sasaki, Y., Martin, D.A. et al. Effect of the dam strain on the spontaneous incidence of cleft lip and palate and intrauterine growth of CL/Fr mouse fetuses. J Assist Reprod Genet 12, 447–452 (1995). https://doi.org/10.1007/BF02211146
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DOI: https://doi.org/10.1007/BF02211146