Skip to main content

Advertisement

SpringerLink
Log in

Comparison of blood and synovial fluid lymphocyte subsets in rheumatoid arthritis and osteoarthritis

  • Published:
Clinical Rheumatology Aims and scope Submit manuscript

Summary

Immunoregulatory T-cell deficiency is thought to underlie pathogenesis of rheumatoid arthritis (RA) as a systemic autoimmunopathy. The aim of this study was a simultaneous analysis of peripheral blood and synovial lymphocyte subsets (Ly-SS) of RA patients as compared to patients with locally active osteoarthritis (OA). Peripheral blood Ly-SS and paired synovial fluid Ly-SS from 87 RA patients were analysed by two dimensional flow cytometry (Simulset Becton Dickinson) as compared to 15 OA patients. The control group consisted of 32 healthy subjects. The peripheral blood analysis from RA and OA patients revealed a significant decrease of CD8+T-cells and increase of CD4+:CD8+ ratio when compared to the control group. The blood of RA patients showed a significant increase of HLA DR+ and IL 2R+T cells as compared to OA group. The synovial fluid from RA and OA patients showed a significant increase of CD3+, CD8+, HLA DR+ T-cells and decrease of CD4+:CD8+ ratio and CD19+ cells in comparison to the peripheral blood. This study shows, that the OA T-cell system seems not to be activated in peripheral blood in opposition to RA patients. Synovial fluid Ly-SS in OA, however, showed only quantitative but not qualitative differences. OA seems to be mainly a local inflammatory response depending on T-cells, when lymphocyte T activity in blood is diminished.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Cush, J.J., Lipsky, P.E. Phenotypic analysis of synovial tissue and peripheral blood lymphocytes isolated of patients with RA. Arthritis Rheum 1988, 31, 1230–1238.

    Google Scholar 

  2. Duke, O., Panayi, G.S., Janossy, G., Poulter, L.W., Tidman, N. Analysis of T cell subsets in the peripheral blood and synovial fluid of patients with rheumatoid arthritis by means of monoclonal antibodies. Ann Rheum Dis 1983, 42, 357–361.

    Google Scholar 

  3. Emery, P., Gentry, K., Mackay, J.R., Muirden, K.D., Rowley, M. Deficiency of the suppressor inducer subset of T lymphocytes in RA. Arthritis Rheum 1987, 30, 849–856.

    Google Scholar 

  4. Janossy, G., Panayi, G., Dunke, O., Bofill, M., Poulter, L.W., Goldstein, G. Rheumatoid arthritis: a disease of T lymphocytes/macrophage immunoregulation. Lancet 1981, 2, 839–842.

    Google Scholar 

  5. Rueda, H.C., Schainberg, H., Diniz, R., Scheinberg, M.A. Helper (OKT4)/suppressor (OKT8) ratios in the peripheral blood and synovial fluid of the patients with rheumatoid arthritis. Clin Exp Rheumatol 1984, 2, 293–296.

    Google Scholar 

  6. Smith, M.D., Roberts-Thomson, P.J. Lymphocytes surface marker expression in rheumatic diseases: evidence for prior activation of lymphocytes in vivo. Ann Rheum Dis 1990, 49, 81–87.

    Google Scholar 

  7. Verwilghen, J., Vertessen, S., Stevens, E.A., Dequeker, J., Ceuppens, J.L. Depressed T cell reactivity to recall antigen in rheumatoid arthritis. J Clin Immunol 1990, 10, 90–98.

    Google Scholar 

  8. haraoui, B., Pelletier, J.P., Cloutier, J.M., Faure, M.P., Martel-Pelletier, J. Synovial membrane histology and immunopathology in rheumatoid arthritis and osteoarthritis. In vivo effects of antirheumatic drugs. Arthritis Rheum 1991, 34, 153–163.

    Google Scholar 

  9. Lindblad, S., Hedfors, E. Arthroscopic and immunohistologic characterization of knee joint synovitis in osteoarthritis. Arthritis Rheum 1987, 30, 1081–1088.

    Google Scholar 

  10. Revell, P.A., Mayston, V., Lalor, P., Mapp, P. The synovial membrane in osteoarthritis: a histological study including the characterization of the cellular infiltrate present in inflammatory osteoarthritis using monoclonal antibodies. Ann Rheum Dis 1988, 47, 300–307.

    Google Scholar 

  11. Reidbord, H.E., Osial, T.A. Synovial lymphocyte subsets in rheumatoid arthritis and degenerative joint disease. J Rheumatol 1987, 14, 1089–1094.

    Google Scholar 

  12. Reme, T., Portier, M., Frayssinoux, F., Combe, B., Miossec, P., Favier, F., Sany, J. T cell receptor expression and activation of synovial lymphocyte subsets in patients with rheumatoid arthritis. Phenotyping of multiple synovial sites. Arthritis Rheum 1990, 33, 485–492.

    Google Scholar 

  13. Arnett, F.C., Edworthy, S.M., Bloch, D.A., McShane, D.J., Fries, J.F., Cooper, N.S., Healey, L.A., Kaplan, S.R., Liang, M.H., Luthra, H.S., Medsger, T.A. Jr., Mitchell, D.M., Neustadt, D.H., Pinals, R.S., Schaller, J.G., Sharp, J.T., Wilder, R.L., Hunder, G.G. The American Rheumatism Association 1987 revised criteria for the classification of rheumatoid arthritis. Arthritis Rheum 1988, 31, 315–324.

    Google Scholar 

  14. Ziff, M. Role of the endothelium in chronic inflammatory synovitis. Arthritis Rheum 1991, 34, 1345–1351.

    Google Scholar 

  15. Hale, L.P., Martin, M.E., McCollum, D.E., Nunley, J.A., Springer, T.A., Singer, K.H., Haynes, B.F. Immunohistologic analysis of the distribution of cell adhesion nodules within the inflammatory synovial microenvironment. Arthritis Rheum 1989, 32, 22–29.

    Google Scholar 

  16. Hanly, J.G., Pledger, D. Characteristics of immunocompetent cells in synovial membranes from multiple sites in patients with rheumatoid arthritis. Ann Rheum Dis 1992, 51, 1066–1068.

    Google Scholar 

  17. Cooper, S.M., Dier, D.L., Roessner, K.D., Budd, R.C., Nicklas, J.A. Diversity of rheumatoid synovial tissue T cells by T cell receptor analysis. Oligoclonal expansion in Interleukin-2-responsive cells. Arthritis Rheum 1991, 34, 537–546.

    Google Scholar 

  18. Jahn, B., Burmester, G.R., Stock, P., Rohwer, P., Kalden, J.R. Functional and phenotypical characterization of activated T cells from intra-articular sites in inflammatory joint disease. Possible modulation of the CD3 antigen. Scand J Immunol 1987, 26, 745–754.

    Google Scholar 

  19. Fox, R., Fong, S., Sabharwal, N., Carstens, S.A., Kung, P.C., Vaughan, J.H. Synovial fluid lymphocytes differ from peripheral blood lymphocytes in patients with rheumatoid arthritis. J Immunol 1982, 128, 351–354.

    Google Scholar 

  20. Crockard, A.D., Finch, M.B., Fay, A.C., McNeil, T.A., Bell, A.L., Roberts, S.D. Comparative analysis of CD4 subsets in peripheral blood and synovial fluid of patients with inflammatory joint disease. Scand J Rheumatol 1990, 19, 157–161.

    Google Scholar 

  21. Jahn, B., Burmester, G.R., Gramatzki, G., Weseloh, G., Stock, P., Kalden, J.R. Interarticular T lymphocytes in monoarticular and oligoarticular inflammatory joint disease. J Rheumatol 1986, 13, 254–258.

    Google Scholar 

  22. Clegg, D.O., Pincus, S.H., Zone, J.J., Ward, J.R. Circulating HLA DR bearing T cells: correlation with the genetic rather than clinical variables. J Rheumatol 1986, 13, 870–874.

    Google Scholar 

  23. Morimoto, C., Letvin, N.L., Distaso, J.A., Aldrich, W.R., Schlossman, S.F. The isolation and characterization of the human suppressor inducer T cell subset. J Immunol 1985, 134, 1508–1515.

    Google Scholar 

  24. Lasky, H.P., Bauer, K., Pope, R.M. Increased helper inducer and decreased suppressor inducer phenotypes in the rheumatoid joint. Arthritis Rheum 1988, 31, 52–59.

    Google Scholar 

  25. Morimoto, C., Romain, P.L., Fox, D.A., Anderson, P., Dimaggio, M., Levine, H., Schlossman, S.F. Abnormalities in CD4+T-lymphocyte subsets in inflammatory rheumatic diseases. Am J Med 1988, 84, 817–825.

    Google Scholar 

  26. Goto, M., Miyamoto, T., Nishioka, K., Uchida, S. T cytotoxic and helper cells are markedly increased, and T suppressor and inducter cells are markedly decreased in rheumatoid synovial fluids. Arthritis Rheum 1987, 30, 737–743.

    Google Scholar 

  27. Corkill, M.M., Kirkham, B.W., Haskard, D.O., Barbatis, C., Gibson, T., Panayi, G.S. Gold treatment of rheumatoid arthritis decreases synovial expression of the endothelial leukocyte. Adhesion receptor ELAM-1. J Rheumatol 1991, 18, 1453–1460.

    Google Scholar 

  28. Karlsson-Parra, A., Svenson, K., Hällgren, R., Klareskog, L., Forsum, U. Peripheral blood T-lymphocyte subsets in active rheumatoid arthritis-effects of different therapies on previously untreated patients. J Rheumatol 1986, 13, 263–268.

    Google Scholar 

  29. Verbruggen, G., Veys, E.M., Hermanns, P., Delanghe, J., Vansteenkiste, M.. The effect of low doses of prednisolone on the traffic of T-helper-inducer cells in rheumatoid arthritis. Clin Rheumatol 1982, 1, 199–207.

    Google Scholar 

  30. Bakke, A.C., Kirland, P.A., Kitridou, R.C., Quismorio, F.P., Rear, J.R.T., Ehresmann, G.R., Horwitz, D.A. T-lymphocyte subsets in SLE. Correlations with corticosteroid therapy and disease activity. Arthritis Rheum 1983, 26, 745–750.

    Google Scholar 

  31. Doita, M., Maeda, S., Kawai, K., Hirohata, K., Sugiyam, A. Analysis of lymphocytes subsets of bone marrow in patients with rheumatoid arthritis by two colour immunofluorescence and flow cytometry. Ann Rheum Dis 1990, 49, 168–171.

    Google Scholar 

  32. Emery, P., Wood, N., Gentry, K., Stockman, A., Mackay, I.R., Bernard, O. High affinity interleukin-2-receptors on blood lymphocytes are decreased during active rheumatoid arthritis. Arthritis Rheum 1988, 31, 1176–1181.

    Google Scholar 

  33. Nouri, A.M.E., Panayi, G.S. Cytokines and the chronic inflammation of rheumatic disease. III. Deficient Interleukin-2 production in rheumatoid arthritis is due to suppressor mechanisms. J Rheumatol 1987, 14, 902–906.

    Google Scholar 

  34. Soden, M., Rooney, M., Whelan, A., Feighery, C. Bresnihan, B. Immunohistological analysis of the synovial membrane: search for predictors of the clinical course in rheumatoid arthritis. Ann Rheum Dis 1991, 50, 673–676.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Rheumatology, Medical School, M. Sklodowska Curie Street 24a, 15-276, Bialystok, Poland

    A. Kuryliszyn-Moskal M.D.

  2. Department of Rheumatology, Dr. Alfons Gamp Str. 1, Bad Kreuznach, Germany

    A. Kuryliszyn-Moskal M.D.

Authors
  1. A. Kuryliszyn-Moskal M.D.
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kuryliszyn-Moskal, A. Comparison of blood and synovial fluid lymphocyte subsets in rheumatoid arthritis and osteoarthritis. Clin Rheumatol 14, 43–50 (1995). https://doi.org/10.1007/BF02208083

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02208083

Key words

Search

Navigation