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Proliferation of peripheral blood mononuclear cells from rheumatoid arthritis patients to mycobacterial antigens

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Summary

Proliferation of rheumatoid and control peripheral blood mononuclear cells (PBMC) to an antigenic acetone-precipitable extract from mycobacterium tuberculosis (MTa) was investigated. Cells were also stimulated with the recall antigen tuberculin PPD (purified protein derivative) and the mitongen OKT3. Controls had a significantly higher response to both MTa and tuberculin PPD than RA patients. However, lymphocytes from patients who had had their disease for 3–10 years profliferated more vigorously to MTa than did PBMD from patients with longer disease duration. There was no difference in the proliferation to OKT3 between patients and controls. We were not able to confirm a previously found correlation between the HLA-DR4 phenotype and lymphocyte transformation to MTa.

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References

  1. Pearson, C.M. Development of arthritis, periarthritis and periostitis in rats given adjuvant. Proc Soc Exp Biol Med 1956, 91, 95–101.

    Google Scholar 

  2. Pearson, C.M., Wood, F.O. Passive transfer of adjuvant arthritis by lymph node or spleen cells. J Exp Med 1964, 120, 547–60.

    Google Scholar 

  3. Holoshitz, J., Matitiau, A., Cohen, IR. Arthritis induced in rats by cloned T-lymphocytes responsive to mycobacteria but not to collagen type II. J Clin Invest 1994, 73, 211–15.

    Google Scholar 

  4. Cohen, I.R., Holoshitz, J., van Eden, W., Frenkel, A. T-lymphocyte clones illuminate pathogenesis and affect therapy of experimental arthritis. Arthritis Rheum 1985, 28, 841–45.

    Google Scholar 

  5. Van Eden, W., Holoshitz, J., Nevo, A., Frenkel, A., Klajman, A., Cohen, I.R. Arthritis induced by a T-lymphocyte clone that responds to Mycobacterium tuberculosis and to cartilage proteoglycans. Proc Natl Acad Sci 1985, 82, 5117–20.

    Google Scholar 

  6. Holoshitz, J., Klajman, A., Drucker, I., Lapidot, Z., Yaretzky, A., Frenkel, A., van Eden, W., Cohen, I.R. T-lymphocytes from rheumatoid arthritis patients show augmented reactivity to a fraction of mycobacteria cross-reactive with cartilage. Lancet 1986, II, 305–309.

    Google Scholar 

  7. Ottenhoff, T.H.M., Torres, P., de las Aguas, J.T., Fernandez, R., van Eden, W., de Vries, R.R.P., Stanford, J.L. Evidence for a HLA-Dr4-associated immune-response gene for Mycobacterium tuberculosis: a clue to the pathogenesis of rheumatoid arthritis? Lancet 1986, II, 310–12.

    Google Scholar 

  8. Palacios-Boix, A.A., Estrada-G., I., Colston, M.J., Panayi, G.S. HLA-DR4 restricted lymphocyte proliferation to a mycobacerium tuberculosis extract in rheumatoid arthritis and healthy subjects. J Immunol 1988, 140, 1844–50.

    Google Scholar 

  9. Ropes, M.W., Bennett, G.A., Cobb, S., Jacox, R., Jessar, R.A. 1958 revision of diagnostic criteria for rheumatoid arthritis. Bull Rheum Dis 1958, 9, 175–76.

    Google Scholar 

  10. Res, P.C., Schaar, C.G., Breedveld, F.C., van Eden, W., van Embden, J.D.A., Cohen, I.R., de Vries, R.R.P. Synovial fluid T-cell reactivity against 65 kD heat shock protein of mycobacteria in early chronic arthritis. Lancet 1988, II, 478–80.

    Google Scholar 

  11. Kingsley, G.H., Pitzalis, C., Panayi, G.S. Abnormal lymphocyte reactivity to self-major histocompatibility antigens in rheumatoid arthritis. J Rheumatol 1987, 14, 667–73.

    Google Scholar 

  12. Emery, P., Panayi, G.S., Nouri, A.M.E. Interleukin-2 reverses deficient cell-mediated immune responses in rheumatoid arthritis. Clin Exp Immunol 1984, 57, 123–29.

    Google Scholar 

  13. Pope, R.M., Pahlavani, M.A., LaCour, E., Sambol, S., Desai, B.V. Antigenic specificity of rheumatoid synovial fluid lymphocytes. Arthritis Rheum 1989, 32, 1371–80.

    Google Scholar 

  14. Van Eden, W., Thole, J.E.R., van der Zee, R., Noordzij, A., van Embden, J.D.A., Hensen, E.J., Cohen, I.R. Cloning of the mycobacterial epitope recognized by T-lymphocytes in adjuvant arthritis. Nature 1988, 331, 171–73.

    Google Scholar 

  15. Emery, P., Panayi, G.S., Welsh, K.I., Cole, B.C. Relationship of HLA-DR4 to defective cellular immunity in rheumatoid arthritis using PPD, and mycoplasma and lectin mitogens. J Rheumatol 1985, 12, 859–64.

    Google Scholar 

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Authors and Affiliations

  1. Rheumatology Unit, Division of Medicine, United Medical and Dental Schools of Guy's and St Thomas' Hospitals, SE1 9RT, London, UK

    M. Swärdh, A. Palacios-Boix & G. S. Panayi

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  1. M. Swärdh
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  2. A. Palacios-Boix
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  3. G. S. Panayi
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Swärdh, M., Palacios-Boix, A. & Panayi, G.S. Proliferation of peripheral blood mononuclear cells from rheumatoid arthritis patients to mycobacterial antigens. Clin Rheumatol 11, 81–85 (1992). https://doi.org/10.1007/BF02207090

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  • DOI: https://doi.org/10.1007/BF02207090

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