Plant and Soil

, Volume 81, Issue 1, pp 111–118 | Cite as

Nitrogen fixation (C2H2 reduction) in soil samples from rhizosphere of rice grown under alternate flooded and nonflooded conditions

  • V. Rao Rajaramamohan
  • J. L. N. Rao


In a greenhouse study the influence of alternate flooded and nonflooded conditions on the N2-ase activity of rice rhizosphere soil was investigated by C2H2 reduction assay. The soil fraction attached to roots represent the rhizosphere soil. Soil submergence always accelerated N2-ase and this effect was more pronounced in planted system. Moreover, rice plant exhibited phase-dependent N2-ase with a maximum activity at 60 days after transplanting. The alternate flooded and nonflooded regimes resulted in alterations of the N2-ase activity. Thus, the N2-ase activity increased following a shift from nonflooded to flooded conditions, but the activity decreased when the flooded soil was returned to nonflooded condition by draining. However, the differential influence of the water regime on N2-ase was not marked in prolonged flooded-nonflooded cycles. Microbial analysis indicated the stimulation of different groups of free-living and associative N2-fixing microorganisms depending on the water regime.

Key words

Flooded Nitrogenase activity Nonflooded Rhizosphere soil Rice 


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  1. 1.
    Alexander M 1974 Introduction to Soil Microbiology. John Wiley and Sons, New York.Google Scholar
  2. 2.
    App A A, Watanabe I, Alexander M, Ventura W, Daez C, Santiago T and De Datta S K 1980 Nonsymbiotic nitrogen fixation associated with rice plant in flooded soils. Soil Sci. 130, 283–289.Google Scholar
  3. 3.
    Boddey R M, Quilt P and Ahmad N 1978 Acetylene reduction in the rhizosphere of rice: Methods of assay. Plant and Soil 50, 567–574.Google Scholar
  4. 4.
    Boddey R M and Dobereiner J 1982 Association of Azospirillum and other diazotrophs with tropical gramineae (28–47).In Non-symbiotic N2 Fixation and Organic Matter in the Tropics. Symposia Paper I, Transactions of the 12th International Congress of Soil Science, New Delhi.Google Scholar
  5. 5.
    Brouzes R C, Mayfield I and Knowles R 1971 Effect of oxygen partial pressure on nitrogen fixation and acetylene reduction in a sandy loam soil amended with glucose. Plant and Soil Spec. Vol. 481–494.Google Scholar
  6. 6.
    Charyulu P B B N and Rao V R 1979 Nitrogen fixation in some Indian rice soils. Soil Sci. 128, 86–89.Google Scholar
  7. 7.
    Charyulu P B B N, Nayak D N and Rao V R 198115N2 incorporation by rhizosphere soil. Influence of rice variety, organic matter and combined nitrogen. Plant and Soil 59, 399–405.Google Scholar
  8. 8.
    De Datta S K 1981 Principles and Practices of Rice Production. John Wiley and Sons, New York.Google Scholar
  9. 9.
    Dei Y and Yamasaki S 1979 Effect of water and crop management on nitrogen supplying capacity of paddy soils. pp 451–463.In Nitrogen and Rice. International Rice Research Institute, Los Banos, Philippines.Google Scholar
  10. 10.
    Garcia J L 1975 Effect rhizosphere due riz sur la denitrification. Soil Biol. Biochem. 7, 139–141.Google Scholar
  11. 11.
    Garcia J L 1975 Evaluation de la denitrification dans les rizieres par la methode de reduction de H2O. Soil Biol. Biochem. 7, 251–256.Google Scholar
  12. 12.
    Habte M and Alexander M 1980 Nitrogen fixation by photosynthetic bacteria in lowland rice culture. Appl. Environ. Microbiol. 39, 342–347.Google Scholar
  13. 13.
    Koyama T and App A 1979 Nitrogen balance in flooded rice soils. pp 95–104.In Nitrogen and Rice. International Rice Research Institute, Los Banos, Philippines.Google Scholar
  14. 14.
    Lee K K, Alimagno B V and Yoshida T 1977 Field technique using acetylene reduction method to assay nitrogenase activity and its association with the rice rhizosphere. Plant and Soil 46, 127–134.Google Scholar
  15. 15.
    Mahapatra R N and Rao V R 1981 Influence of hexachlorocyclohexane on the nitrogenase activity of rice rhizosphere soil. Plant and Soil 59 473–477.Google Scholar
  16. 16.
    Nayak D N and Rao V R 1977 Nitrogen fixation bySpirillum sp. from rice roots. Arch. Microbiol. 115, 358–359.Google Scholar
  17. 17.
    Nayak D N and Rao V R 1981 The influence of alternate flooded and nonflooded conditions on nitrogen fixation (C2H2 reduction) in paddy soils. Soil Sci. 131, 26–29.Google Scholar
  18. 18.
    Okon Y, Albrecht S L and Burris R H 1977 Methods for growingSpirillum lipoferum and for counting it in pure culture and in association with plants. Appl. Environ. Microbiol. 33, 85–88.Google Scholar
  19. 19.
    Patrick W H and Mahapatra I C 1968 Transformation and availability to rice of nitrogen and phosphorus in waterlogged soils. Adv. Agron. 20, 323–329.Google Scholar
  20. 20.
    Rao V R, Kalininskaya T A and Miller U M 1973 The activity of non-symbiotic nitrogen fixation in soils of rice fields studied with15N. Microbiologiya 42, 729–734.Google Scholar
  21. 21.
    Reddy K R and Patrick W H 1975 Effect of alternate aerobic and anaerobic conditions on redox potential, organic matter decomposition and nitrogen loss in a flooded soil. Soil Biol. Biochem. 7, 87–94.Google Scholar
  22. 22.
    Tam T Y, Mayfield C I and Inniss W E 1981 Nitrogen fixation and methane metabolism in a stream sediment water system amended with leaf material. Can. J. Microbiol. 27, 511–516.PubMedGoogle Scholar
  23. 23.
    Tenny F G and Waksman S A 1930 Composition of natural organic materials and their decomposition in the soil. 5. Decomposition of various chemical constituents in plant material under anaerobic conditions. Soil Sci. 30, 143–160.Google Scholar
  24. 24.
    Watanabe I, Lee K K and Deguzman M R 1978 Seasonal changes in nitrogen-fixing rate in lowland rice field assayed byin situ acetylene reduction technique. 2. Estimation of nitrogen fixation associated with rice plant. Soil Sci. Pl. Nutr. 24 465–471.Google Scholar
  25. 25.
    Yoshida T and Ancajas R R 1973 Nitrogen-fixing activity in upland and flooded rice fields. Soil Sci. Soc. Am. Proc. 37 42–46.Google Scholar

Copyright information

© Martinus Nijhoff/Dr W. Junk Publishers 1984

Authors and Affiliations

  • V. Rao Rajaramamohan
    • 1
  • J. L. N. Rao
    • 1
  1. 1.Division of Soil Science and MicrobiologyCentral Rice Research InstituteCuttackIndia

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