Summary
The effect of soil acidity on spore germination, germ tube growth and root colonization of vesicular-arbuscular mycorrhizal (VAM) fungi was examined using a Florida Ultisol. Soil samples were treated with 0, 4, 8 and 12 meq Ca/MgCO3/100 g soil and each lime level received 0, 240, and 720 ppm P as superphosphate. Corn (Zea mays L.) was planted in the soil treatments, inoculated with eitherGlomus mosseae orGigaspora margarita spores and grown for 31 days. Acid soil inhibits mycorrhizal formation byG. mosseae through its strong fungistatic effect against the spores. The dolomitic lime increased mycorrhizal formation by both fungal species.G. margarita is much less sensitive to acidic conditions thanG. mosseae. Al ions are a very important component of the fungistatic property against the VAM symbiosis. VAM fungus adaptation may be important for plants growing on infertile acid soils if soil inoculation with these fungi is to contribute significantly to low-input technology for tropical agricultural systems.
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References
Abbott L K and Robson A D 1977 The distribution and abundance of vesicular-arbuscular endophytes in some Australian soils. Aust. J. Bot. 25, 515–522.
Bowen G D 1980 Mycorrhizal roles in tropical plants and ecosystems.In Tropical Mycorrhiza Research. Ed. P M, Kola. Oxford University Press, Oxford, pp 165–190.
Daft M J, Hacskaylo E and Nicolson T M 1975 Arbuscular mycorrhizas in plants colonising coal spoils in Scotland and Pennsylvania.In Endomycorrhizas. Eds. F E Sanders, B Mosse and P B Tinker. Acad Press, London, pp 581–592.
Davis E A, Young J L and Liderman R G 1983 Soil lime level (pH) and VA-mycorrhiza effects on growth responses of sweetgum seedlings. Soil Sci. Soc. Am. J. 47, 251–256.
Elmes R, Mosse B and O'Shea J 1980 Nutrient film technique. Rothamsted Exp. Sta. Report pp 203–204.
Gildon A and Tinker P B 1981 A heavy metal-tolerant strain of a mycorrhizal fungus. Trans. Brit. Mycol. Soc. 77, 648–649.
Graw D 1979 The influence of soil pH on the efficiency of vesicular-arbuscular mycorrhiza. New Phytol. 82, 687–695.
Green N E, Graham S O and Schenck N C 1976 The influence of pH on the germination of vesicular-arbuscular mycorrhizal spores. Mycologia 68, 929–934.
Hepper C M 1979 Germination and growth ofGlomus caledonius spores: the effects of inhibitors and nutrients. Soil Biol. Biochem. 11, 269–277.
Hepper C M and Smith G A 1976 Observations on the germination ofEndogone spores. Trans. Br. Mycol. Soc. 66, 189–194.
Hora T S and Baker R 1974 Abiotic generation of volatile fungistatic factor in soil by liming. Phytopathology 74, 624–629.
Hubbell D H 1971 Microbiological effects from liming soils. Soil Crop Sci. Soc. Florida 31, 196–199.
Ko W H and Hora F K 1972 Identification of an Al ion as a soil fungitoxin. Soil Sci. 113, 42–45.
Lambert D H and Cole H Jr 1980 Effects of mycorrhizae on establishment and performance of forage species in mine spoil. Agron. J. 72, 257–260.
Mahmud A W 1983 Response of corn (Zea mays L.) to inoculation with vesicular-arbuscular mycorrhizal fungi in a phosphorus-deficient soil. Ph.D. thesis, University of Florida, Gainesville, USA.
McIlveen W D and Cole H 1978 Influence of zinc on development of the endomycorrhizal fungusGlomus mosseae and its mediation on P uptake byGlycine max. “Amsoy 71”. Agric. Environ. 4, 245–256.
Mosse B 1081 Vesicular-arbuscular mycorrhiza research for tropical agriculture. Hawaii Inst. for Tropical Agric. and Human Resources. Research Bulletin, 194, 82 p.
Mosse B 1972 Effects of differentEndogone strains on the growth ofPaspalum notatum. Nature London 239, 221–223.
Mosse B 1972 The influence of soil type andEndogone strain on the growth of mycorrhizal plants in phosphorus deficient soils. Rev. Ecol. Biol. Sol. 9, 529–537.
Phillips J M and Hayman D S 1970 Improved procedures for clearing roots and straining parasitic and vesicular-arbuscular mycorrhizal fungi for rapid assessment of infection. Trans. Br. Mycol. Soc. 55, 158–161.
Read D J, Koucheki H K and Hodgson J 1976 Vesicular-arbuscular mycorrhiza in natural vegetation systems. I. The occurrence of infection. New Phytol. 77, 641–653.
Reeve N G and Sumner M E 1970 Lime requirements of Natal Oxisols based on exchangeable aluminium. Soil Sci. Soc. Am. Proc. 34, 595–598.
Sanchez P A and Salinas J G 1981 Low-input technology for managing Oxisols and Ultisols in tropical America. Adv. Agron. 34, 279–406.
Siqueira J O 1983 Nutritional and edaphic factors affecting spores germination, germ tube growth, and root colonization by the vesicular-arbuscular mycorrhizal fungi. Ph.D. dissertation, University of Florida, Gainesville, USA.
Siqueira J O, Hubbell D H and Schenck N C 1982 Spore germination and germ tube growth of a vesicular-arbuscular mycorrhizal fungusin vitro. Mycologia 74, 952–959.
Sparling G P and Tinker P B 1978 Mycorrhizal infection in Pennine grassland. I. Level of infection in the field. J. Appl. Ecol. 15, 943–950.
Yawney W I, Schultz R C and Kormanik P P 1982. Soil phosphorus and pH influence the growth of mycorrhizal sweetgum. Soil Sci. Am. J. 46, 1315–1320.
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Siqueira, J.O., Hubbell, D.H. & Mahmud, A.W. Effect of liming on spore germination, germ tube growth and root colonization by vesicular-arbuscular mycorrhizal fungi. Plant Soil 76, 115–124 (1984). https://doi.org/10.1007/BF02205572
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DOI: https://doi.org/10.1007/BF02205572