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Simultaneous mass fragmentographic determination of 3, 4-dihydroxyphenylacetic acid and 4-hydroxy-3-methoxyphenylacetic acid in brain tissue

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Summary

A massfragmentographic method for determination of 3, 4-dihydroxyphenylacetic acid (DOPAC) is described. Deuterium labelled DOPAC was used as internal standard. Derivatives suitable for GC-MS were prepared by reacting standard solutions and rat brain extracts with pentafluoropropanol and pentafluoropropionic anhydride. Fragments set into focus were m/e 592/597 (M+) and m/e 415/420 (M+ −177). The sensitivity was in the pmole range with high specificity as tested by multiple ion analysis. The DOPAC level in rat striatum was 6.0 ± 1.1 nmoles/g with an experimental error below 8.5%. The recovery of 575 pmoles DOPAC added to brain extracts was 91 %. Treatment of rats with pargyline (150 mg/kg i.p.) reduced the DOPAC level by about 95% whereas a slight elevation was obtained after administration of chlorpromazine (10 mg/kg i.p.). DOPAC and 4-hydroxy-3-methoxyphenylacetic acid (HVA) could be determined concomitantly in the same sample, by using the described technique for derivative preparation and an AVA allowing simultaneous recording of 4 mass numbers.

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Authors and Affiliations

  1. Division of Neuropsychopharmacology, Department of Pharmacology, Karolinska Institutet, Stockholm, Sweden

    F. -A. Wiesel, C. -G. Fri & G. Sedvall

Authors
  1. F. -A. Wiesel
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  2. C. -G. Fri
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  3. G. Sedvall
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With 4 Figures

Part of the study was presented as a preliminary report at the 2nd International Symposium on Gas Chromatography Mass Spectrometry, Milan, Italy, 1974.

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Wiesel, F.A., Fri, C.G. & Sedvall, G. Simultaneous mass fragmentographic determination of 3, 4-dihydroxyphenylacetic acid and 4-hydroxy-3-methoxyphenylacetic acid in brain tissue. J. Neural Transmission 35, 319–326 (1974). https://doi.org/10.1007/BF02205228

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  • DOI: https://doi.org/10.1007/BF02205228

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