Plant and Soil

, Volume 62, Issue 1, pp 65–80 | Cite as

Phyllosphere and rhizosphere microflora of pearl millet with reference to downy mildew incited bySclerospora graminicola (Sacc.) Schroet

  • C. Promod Chandra Kumar
  • K. A. Balasubramanian


Analysis of phyllosphere microflora showed that in the resistant cultivar (PHB-14) there was a significantly higher population of fungi, gram positive and gram negative bacteria, compared to susceptible cultivar (NHB-3) under healthy and diseased situations. The cultivars during earhead stage supported maximum phyllosphere fungal and gram negative bacterial populations.

The rhizosphere of the susceptible cultivar under downy mildew influence supported maximum fungal and gram negative bacterial populations and the rhizosphere effect was the highest in downy mildew colonized susceptible NHB-3 at the earhead stage (95 days after planting).

Among the fungi isolated Cladosporium dominated the phyllosphere of resistant, susceptible and susceptible cultivar under the influence of downy mildew.

Among the rhizosphere microflora the population ofAspergillus sp. was found to be greatest in resistant, susceptible and the susceptible cultivar under the influence of downy mildew.

Key Words

Downy mildew Microflora Phyllosphere Rhizosphere 


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  1. 1.
    Agnihothrudu, V. 1961 Rhizosphere microflora of tea (Camellia sinensis (L.) O. Kuntz) in relation to root rot caused byUstulina zonata Leb. Sacc. Soil Sci.91, 133–137.Google Scholar
  2. 2.
    Balasubramanian, K. A. 1974 Pattern of electrolyte leakage in downy mildew affected sorghum. Experientia30, 1020.Google Scholar
  3. 3.
    Balasubramanian, K. A. 1981 Distribution pattern of micronutrients and exchangeable cations in the rhizosphere of pearl millet under downy mildew disease pressure. Plant and Soilsubmitted.Google Scholar
  4. 4.
    Balagopal, C. and Oblisami, G. 1973 The phyllosphere microflora ofPhaseolus vulgaris L. infected with common bean mosaic virus. Madras Agric. J.60, 478–480.Google Scholar
  5. 5.
    Bailey, J. A. 1971 Phytoalexins and the ability of leaf tissues to inhibit fungal growth.In Ecology of Leaf Surface Microorganisms. Ed. T. F. Preece and C. H. Dickinson. Academic Press, London. pp 519–528.Google Scholar
  6. 6.
    Bhatt, D. D. and Vaughan, E. K. 1963 Interrelationships among fungi associated with strawberries in Oregon. Phytopathology53, 217–220.Google Scholar
  7. 7.
    Blakeman, J. P. 1972 Effect of plant age on inhibition ofBotrytis cinerea spores by bacteria on beet root leaves. Physiol. Plant Pathol.2, 143–152.Google Scholar
  8. 8.
    Bruehl, G. W. and Lai, P. 1966 Prior colonization as a factor in the saprophytic survical of several fungi in wheat straw. Phytopathology56, 766–768.Google Scholar
  9. 9.
    Cruickshank, I. A. M. and Perrin, D. R. 1963 Studies on phytoalexins. VI The effect of some factors on its formation inPisum sativum L. and the significance of pisatin in disease resistance. Aust. J. Biol. Sci.16, 111–128.Google Scholar
  10. 10.
    Cruickshank, I. A. M. and Perrin, D. R. 1965 Studies on Phytoalexin. VIII. The effect of some further factors in the formation, stability and localization of pisatinin vivo. Aust. J. Biol. Sci.18, 817–828.Google Scholar
  11. 11.
    Collins, M. A. 1976 Colonization of leaves by phyllosphere saprophytes and their interaction in this environment.In Microbiology of Aerial Plant Surfaces. Eds. C. H. Dickinson and T. F. Preece. Academic Press, London. pp 401–418.Google Scholar
  12. 12.
    Dickinson, C. H. 1978 Factors affecting microbial growth in the phylloplane. Third International Congress of Plant Pathology, München Abstract. 102 p.Google Scholar
  13. 13.
    Dickinson, C. H. 1976 Fungi on the aerial surfaces of higher plants.In Microbiology of Aerial Plant Surfaces. Eds. C. H. Dickinson and T. F. Preece. Academic Press London. pp 293–324.Google Scholar
  14. 14.
    Diem, H. G. 1969 Microorganisms de la surface des feuilles. II Interactions entre quelques champignins parasites et dives saprophytes filamenteux de la phyllosphere de l'orge. Bull. Ec. Nat. Super. Agron. Nancy11, 12–17.Google Scholar
  15. 15.
    Fokkema, N. J. 1968 The influence of pollen in the development ofCladosporium herbarum in the phyllosphere of rye. Neth. J. Plant Pathol.74, 159–165.Google Scholar
  16. 16.
    Fokkema, N. J. 1973 The role of saprophytic fungi in antagonism againstDrechslera sorokiniana (Helminthosporium sativum) on agar plates and on rye leaves with pollen. Physiol. Plant Pathol.3, 195–205.Google Scholar
  17. 17.
    Fokkema, N. J., Laar, J. A. J., Van de Nelis Blomberg, A. L. and Schippers, B. 1975 The buffering capacity of the natural mycoflora of rye leaves to infection byCochliobolus sativus and its susceptibility to benomyl. Neth. J. Plant Pathol.81, 176–186.Google Scholar
  18. 18.
    Garbutt, J. W. and Bartlett, A. J. 1972 Experimental Biology with Microorganisms Students Manual, Butterworth, London 180 p.Google Scholar
  19. 19.
    Harper, J. L. 1950 Studies on the resistance of certain varieties of banana to Panama disease II Rhizosphere. Plant and Soil2, 374–394.Google Scholar
  20. 20.
    Hiltner, L. 1904 Über neure Erfahrungen und Probleme auf dem Gebiet der Bodenbakteriologie und unter besonderer Berucksichtigung der Grunddungung und Brache. Arb. Dtsch. Landwirtsch. Ges.98, 59–78.Google Scholar
  21. 21.
    Hocking, D. 1968 Fungi associated with damping off and healthy pine seedlings and with seed in East African pine nurseries. Trans. Br. Mycol. Soc.51, 221–226.Google Scholar
  22. 22.
    Hornby, D. and Ullstrup, A. J. 1967 Fungal populations associated with maize roots. Quantitative rhizosphere data for genotypes differing in root rot resistance. Phytopathology57, 76–82.Google Scholar
  23. 23.
    Johnson, L. F. and Curl, E. A. 1972 Methods of Research on the Ecology of Soil Borne Plant Pathogens. Burgess Publication, Minneapolis. 247 p.Google Scholar
  24. 24.
    Katznelson, H. 1965 Nature and Importance of the rhizosphere.In Ecology of Soil Borne Plant Pathogens. University of California Press. pp 187–209.Google Scholar
  25. 25.
    Ko, W. H. and Lockwood, J. L. 1968 Soil fungistasis: Relation to fungal spore germination. Phytopathology57, 894–901.Google Scholar
  26. 26.
    Ko, W. H. and Lockwood, J. L. 1970 Mechanism of lysis of fungal mycelia in soil. Phytopathology60, 148–154.Google Scholar
  27. 27.
    Lakshmi Kumari, M. 1964 Rhizosphere microflora ofDolichos infected with DEMV. Proc. Indian Acad. Sci.60, 116–127.Google Scholar
  28. 28.
    Last, F. T. 1971 Introduction.In Ecology of Leaf Surface Microorganisms. Eds. T. F. Preece and C. H. Dickinson. Academic Press London.Google Scholar
  29. 29.
    Last, F. T. 1955 Seasonal incidence of Sporobolomyces on cereal leaves. Trans. Br. Mycol. Soc.38, 221–239.Google Scholar
  30. 30.
    Lapp, N. W. and Fairfax, J. A. W. 1976 The role of acid rains as a regulator of folian nutrient uptake and loss.In Microbiology of Aerial Plant Surfaces. Eds. C. H. Dickinson and T. F. Preece. Academic Press, London pp 107–118.Google Scholar
  31. 31.
    Leben, C. 1964 Influence of bacteria isolated from healthy cucumber leaves on two leaf diseases of cucumber. Phytopathology54, 405–408.Google Scholar
  32. 32.
    Lochhead, A. G., Timonin, M. I. and West, P. M. 1940 The microflora of the rhizosphere in relation to resistance of plants to soil borne pathogens. Sci. Agric.20, 414–418.Google Scholar
  33. 33.
    Mishra, R. R. and Kamal, A. 1970 Rhizosphere mycoflora of virus infected plants. Indian Phytopathol.23, 27–31.Google Scholar
  34. 34.
    Mishra, R. R. and Tewari, R. P. 1976 Studies on biological control ofPuccinia graminis tritici.In Microbiology of Aerial Plant Surfaces. Eds. C. H. Dickinson and T. F. Preece. Academic Press, London pp 559–567.Google Scholar
  35. 35.
    Newhook, F. J. 1951 Microbiological control ofBotrytis cinerea Pers. II. Antagonism by fungi and actinomycetes. Ann. Appl. Biol.38, 185–202.Google Scholar
  36. 36.
    Newhook, F. J. 1957 The relationship of saprophytic antagonism to control ofBotrytis cinerea Pers. on tomatoes. N.Z.J. Sci. Technol.38, 473–481.Google Scholar
  37. 37.
    Norse, D. 1972 Fungal population of tobacco leaves and their effect on growth ofAlternaria longipes. Trans. Br. Mycol. Soc.59, 261–271.Google Scholar
  38. 38.
    Preece, T. F. and Dickinson, C. H. 1971 Ecology of Leaf Surface Microorganisms. Academic Press, London 640 p.Google Scholar
  39. 39.
    Pace, N. A. and Campbell, R. 1974 The effect of saprophytes on infection of leaves of Brassica sp. byAlternaria brassicicola. Trans. Br. Mycol. Soc.63, 193–196.Google Scholar
  40. 40.
    Rajkumar and Gupta, J. S. 1974 Phyllosphere microflora of three potato varieties in relation to micro-climatic and meteorological factors. Indian Phytopathol.29, 164–168.Google Scholar
  41. 41.
    Riviere, J. 1959 Contribution to the study of wheat rhizosphere. Ann. Agron.45, 93–337.Google Scholar
  42. 42.
    Rombouts, J. E. 1953 The microorganisms in the rhizosphere of banana in relation to susceptibility or resistance to Panama disease. Plant and Soil4, 276–288.Google Scholar
  43. 43.
    Rovira, A. D. 1965 Plant root exudates and their influence upon soil microorganisms.In Ecology of Soil Borne Plant Pathogens. University of California Press. pp 170–186.Google Scholar
  44. 44.
    Rovira, A. D. 1969 Plant root exudates. Bot. Rev.35, 35–57.Google Scholar
  45. 45.
    Ruinen, J. 1956 Occurrence of Beijerinkia in the phyllosphere. Nature London177, 220–221.Google Scholar
  46. 46.
    Sharma, K. R. and Mukherji, K. G. 1976 Microbial ecology ofSesamum orientale L. andGossypium hirsutum L.In Microbiology of Aerial Plant Surfaces. Eds. C. H. Dickinson and T. F. Preece. Academic Press.Google Scholar
  47. 47.
    Sharma, J. K. and Sinha, S. 1972 Studies on the phyllosphere microflora of Sorghum in relation to anthracnose caused byColletotrichum gaminicola Ces) Wilson. Sorghum Newslett.15, 28–30.Google Scholar
  48. 48.
    Singh, A. B. 1972 Effect of virus infection on the rhisosphere mycoflora of papaya plants. Plant and Soil36, 205–208.Google Scholar
  49. 49.
    Srivastava, V. B. and Mishra, R. R. 1971 Rhizosphere fungal variation in susceptible and healthy wheat. Indian Phytopathol.24, 784–786.Google Scholar
  50. 50.
    Stenlid, G. 1958 Salt loss and redistribution of salts in higher plants. Encycl. Plants Physiol.4, 615–637.Google Scholar
  51. 51.
    Strzelczyk, E. 1958 The influence of various crop plants on the development of Azotobacter and Clostridium in their rhizosphere. Acta Microbiol. Pol.7, 115–123.Google Scholar
  52. 52.
    Subba Rao, N. S. and Bailey, D. L. 1961 Rhizosphere studies in relation to varietal resistance or susceptibility of tomato to Verticillium wilt. Can. J. Bot.39, 1747–1758.Google Scholar
  53. 53.
    Timonin, M. I. 1966 Rhizosphere effect of healthy and diseased lodge pole pine seedlings. Can. J. Microbiol.12, 531–537.Google Scholar
  54. 54.
    Tuckey, H. B. 1971 Leaching of substances from plants.In Ecology of Leaf Surface Microorganisms. Eds. T. F. Preece and C. H. Dickinson. Academic Press, London pp 76–80.Google Scholar
  55. 55.
    Vagnerova, K., Macura, J. and Catska, V. 1960 Rhizosphere microflora of wheat. I. Composition and properties of bacterial flora during the first stage of growth. Folia Microbiol. Prague5, 298–310.Google Scholar
  56. 56.
    Vagnerova, K., Macura, J. and Catska, V. 1960 Rhizosphere microflora of wheat. II. Composition and properties of bacterial flora during vegetative period of wheat. Folia Microbiol. Prague5, 311–319.Google Scholar
  57. 57.
    Valacon, C. R. S. and Lodge, E. 1970 Auxins and other compounds ofCladosporium herbarum. Trans. Br. Mycol. Soc.55, 9–15.Google Scholar
  58. 58.
    Viswanath, N. R., Patil, R. B. and Rangaswamy, G. 1969 Rhizosphere microflora of coffee plants affected by decline disease. Curr. Sci.38, 497–498.Google Scholar
  59. 59.
    Warren, R. C. 1972 The effect of pollen on the fungal leaf microflora ofBeta vulgaris L. and infection of leaves byPhoma betae. Neth. J. Plant Pathol.78, 89–98.Google Scholar
  60. 60.
    Warren, R. C. 1976 Microbes associated with buds and leaves: Some recent investigations on decidous trees.In Microbiology of Aerial Plant Surfaces. Eds. C. H. Dickinson and T. F. Preece. Academic Press, London pp 361–374.Google Scholar

Copyright information

© Martinus Nijhoff/Dr. W. Junk Publishers 1981

Authors and Affiliations

  • C. Promod Chandra Kumar
    • 1
  • K. A. Balasubramanian
    • 1
  1. 1.Department of Plant PathologyA.P. Agricultural UniversityHyderabadIndia

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