Skip to main content
SpringerLink
Log in

Morphologische und funktionelle Veränderungen im Blutzellsystem des Meerschweinchens nach Behandlung mit cytostatischen Substanzen, Prednisolon und Phenylbutazon

III. Veränderungen einer immunologischen und einer unspezifischen Entzündungsreaktion

Morphological and functional alterations of the blood cell system of guinea pigs after treatment with cytostatic agents, prednisolone and phenylbutazone

III. Alterations of an immunologic and an unspecific form of inflammation

  • Published:
Research in Experimental Medicine

Summary

Guinea pigs, having developed cutaneous tuberculin hypersensitivity after immunization with BCG were treated for 10 days by intraperitoneal injections of moderately toxic doses of cyclophosphamide, triethylene melamine, ibenzmethyzin, 6-MP, amethopterin (6 injections in 13 days), actinomycin C, vinblastine, podophyllin derivative, prednisolone and phenylbutazone. After 5 days of treatment tuberculin reaction was suppressed in most animals by 6-MP, whereas the other substances had only moderate or no effect. After 10 days of treatment, however, tuberculin reactions were markedly diminished by nearly all applied substances with exception of vinblastine, TEM and amethopterin, which caused only weak effects. Inspite of nearly complete inhibition of the tuberculin reaction at the end of treatment with 6-MP and ibenzmethyzin a state of tolerance never developed. There was a correlation between the intensity of tuberculin reaction and the number of small lymphocytes with a pale cytoplasm in the blood. An unspecific inflammation, Rebuck's skin window (like the tuberculin reaction characterized by an intensive mononuclear cell infiltration), caused simultaneously with the tuberculin reaction immediately after the period of treatment was more or less markedly diminished by 6-MP, TEM, cyclophosphamide, ibenzmethyzin, podophyllin derivative and actinomycin. The notably good inhibition of an immunological reaction of the delayed type by a large scale of different substances in our experiments is explained by an immunosuppressive effect of all and an anti-inflammatory effect of some substances and by some kind of immunoparalysis caused by frequent injections of antigen.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Literatur

  1. Aisenberg, A. C.: Nature (Lond.)200, 484 (1963).

    Article  CAS  Google Scholar 

  2. Arnason, B. G., andB. H. Waksman: Fortschr. Tuberk.-Forsch.13, 1–97 (1964).

    Google Scholar 

  3. Baker, R., R. Gordon, J. Huffer, andG. H. Miller, Jr.: Arch. Surg.65, 702–705 (1952).

    Article  CAS  Google Scholar 

  4. Begemann, H.: Ärztl. Fortbild.17 (1968) (im Druck).

  5. Berenbaum, M. C.: Nature (Lond.)196, 384–385 (1962).

    Article  CAS  Google Scholar 

  6. —: Nature (Lond.)198, 606–607 (1963).

    Article  CAS  Google Scholar 

  7. —, andJ. N. Brown: Nature (Lond.)200, 84 (1963).

    Article  CAS  Google Scholar 

  8. Billingham, R. E., P. L. Krohn, andP. B. Medawar: Brit. med. J.1951 I, 1157–1163.

    Article  CAS  Google Scholar 

  9. Bohrod, M. G.: Progr. Allergy4, 31–78 (1954).

    Google Scholar 

  10. Bollag, W.: Experientia (Basel)19, 304–307 (1963).

    Article  CAS  Google Scholar 

  11. Borel, Y., andR. Schwartz: J. Immunol.92, 754–761 (1964).

    CAS  PubMed  Google Scholar 

  12. Brodehl, J.: Z. Immun.-Forsch.113, 343 (1956).

    CAS  Google Scholar 

  13. Condie, R. M., W. J. Mennis, andC. Miller: Fed. Proc.20, 26 (Abstr.) (1961).

    Google Scholar 

  14. Cline, M. J.: Blood30, 176–188 (1967).

    CAS  PubMed  Google Scholar 

  15. Dammin, G. J., andS. C. Bukantz: J. Amer. med. Ass.139, 358–362 (1949).

    Article  CAS  Google Scholar 

  16. Domenjoz, R.: Pharmakologie der nichthormonellen Antiphlogistika. InR. Heister u.H. F. Hofmann (Herausgeb.): Die Entzündung, S. 244–252. München-Berlin-Wien: Urban & Schwarzenberg 1966.

    Google Scholar 

  17. Dougherty, T. F.: Progr. Allergy4, 319–360 (1954).

    Google Scholar 

  18. Etteldorf, J. N., S. Roy, R. L. Summitt, M. J. Sweeney, H. P. Wall, andW. M. Berton: J. Pediat.70, 758–766 (1967).

    Article  CAS  PubMed  Google Scholar 

  19. Favez, G.: The influence of oxyphenbutazone on specific circulating antibodies during active tuberculosis in man. InJ. Lindner u.E. Wilhelmi (Herausgeb.): Die infektiöse Entzündung, S. 383–385. Bern-Stuttgart: Huber 1968.

    Google Scholar 

  20. Floersheim, G. L.: Experientia (Basel)19, 546–547 (1963).

    Article  CAS  Google Scholar 

  21. —: Helv. physiol. pharmacol. Acta22, C 121-C 123 (1964).

    Google Scholar 

  22. - In S.Garattini and M. N. G.Dukes (Ed.): Non-steroidal anti-inflammatory drugs, pp. 232–235. Amsterdam-New York-London-Milan-Tokyo-Buenos Aires 1965.

  23. Follis, R. H., Jr.: Bull. Johns Hopk. Hosp.63, 283–299 (1938).

    Google Scholar 

  24. Fukase, M.: Tohoku J. exp. Med.89, 349–362 (1966).

    Article  CAS  PubMed  Google Scholar 

  25. Gell, P. G. H., andB. Benacerrat: Advanc. Immunol.1, 319–343 (1961).

    Article  CAS  Google Scholar 

  26. —, andI. T. Hinde: Bull. schweiz. Äkad. med. Wiss.8, 200–202 (1952).

    CAS  PubMed  Google Scholar 

  27. Genghof, D. S., andJ. R. Battisto: Proc. Soc. exp. Biol. (N.Y.)107, 933–936 (1961).

    Article  CAS  Google Scholar 

  28. Gillissen, G.: Klin. Wschr.43, 590–597 (1965).

    Article  CAS  PubMed  Google Scholar 

  29. Gowans, J. L., andD. D. McGregor: Progr. Allergy9, 1–78 (1965).

    Google Scholar 

  30. Gubner, R., S. August, andV. Ginsberg: Amer. J. med. Sci.221, 176–182 (1951).

    Article  CAS  PubMed  Google Scholar 

  31. Hektoen, L., andH. J. Corper: J. infect. Dis.28, 279–285 (1921).

    Article  Google Scholar 

  32. Hersh, E. M., V. G. Wong, andE. J. Ferireich: Blood27, 38–48 (1966).

    CAS  PubMed  Google Scholar 

  33. Hitchings, G. H., andG. B. Elion: Pharmacol. Rev.15, 365–405 (1963).

    CAS  PubMed  Google Scholar 

  34. Hoffmann, E.: Tuberk.-Arzt9, 464–472 (1955).

    CAS  Google Scholar 

  35. Hoyer, J., andR. M. Condie: Fed. Proc.21, 277 (Abstr.) (1962).

    Google Scholar 

  36. Hoyer, L. W., R. A. Good, andR. M. Condie: J. exp. Med.116, 311–327 (1962).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  37. Hoyer, J. R., L. W. Hoyer, R. A. Good, andR. M. Condie: J. exp. Med.116, 679–685 (1962).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  38. Humphreys, S. R., M. A. Chirigos, K. L. Milstead, N. Mantel, andA. Goldin: J. nat. Cancer Inst.27, 259–276 (1961).

    CAS  PubMed  Google Scholar 

  39. Hunstein, W., u.M. Strey: Klin. Wschr.43, 52–53 (1965).

    Article  CAS  PubMed  Google Scholar 

  40. Jones, J. W., R. Oneal, R. Haines, andG. Rosin: Fed. Proc.21, 40 (1962).

    Google Scholar 

  41. Kaboth, W., F. Trepel, P. Schick, G. Wernekke u.H. Begemann: Z. ges. exp. Med.148, 306–336 (1968).

    Article  CAS  Google Scholar 

  42. Kistler, H.: Int. Arch. Allergy10, 361–375 (1957).

    Article  CAS  PubMed  Google Scholar 

  43. Kosunen, T. U., W. S. Tihen, andB. H. Waksman: Fed. Proc.21, 274 (1962).

    Google Scholar 

  44. Kuwert, E.: Der Einfluß von Oxyphenylbutazon und Phenylbutazon auf den Ablauf der experimentellen Immunencephalitis. InJ. Lindner u.E. Wilhelmi: Die infektiöse Entzündung, S. 117–126. Bern-Stuttgart: Huber 1968.

    Google Scholar 

  45. Lagrue, G., B. N. Halpern, J. Bariéty, P. Samarcg, A. Fray etP. Milliez: C.R. Soc. Biol. (Paris)159, 322 (1965).

    CAS  Google Scholar 

  46. Lange, A.: Z. Rheumaforsch.20, 203–208 (1961).

    CAS  Google Scholar 

  47. LaPlante, E. S., R. M. Condie, andR. A. Good: J. Lab. clin. Med.59, 542–549 (1962).

    CAS  PubMed  Google Scholar 

  48. Leder, L.-D., u.R. Nicolas: Frankfurt. Z. Path.72, 632 (1963).

    CAS  PubMed  Google Scholar 

  49. Long, D. A., andP. C. Spensley: Lancet1954 I, 645–647.

    Article  Google Scholar 

  50. Lorenzen, I., andA. Videbaek: Lancet1965 II, 558–561.

    Article  Google Scholar 

  51. Maguire, H. C., Jr., andH. J. Maibach: J. Invest. Derm.37, 427–431 (1961).

    Article  CAS  PubMed  Google Scholar 

  52. Maguire, H., andE. Steers: Experientia (Basel)19, 591–592 (1963).

    Article  CAS  Google Scholar 

  53. McCluskey, R. T., B. Benacerraf, andJ. W. McCluskey: J. Immunol.90, 466–477 (1963).

    CAS  PubMed  Google Scholar 

  54. McQuarrie, D. G., R. M. Condie, W. R. Meeker, F. Roller, andR. L. Varco: Proc. Soc. exp. Biol. (N.Y.)103, 278–282 (1960).

    Article  CAS  Google Scholar 

  55. Medawar, P. B., andE. M. Sparrow: J. Endocr.14, 240–256 (1956).

    Article  CAS  PubMed  Google Scholar 

  56. Meier, R., u.F. Gross: Dtsch. med. Wschr.76, 1179–1181 u. 1241–1243 (1951).

    Article  CAS  PubMed  Google Scholar 

  57. Moeschlin, S., R. Báguena, andJ. Báguena: Int. Arch. Allergy4, 83–100 (1953).

    Article  CAS  PubMed  Google Scholar 

  58. Najarian, J. S., andJ. D. Feldman: J. exp. Med.114, 779–789 (1961).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  59. Page, A. R., R. M. Condie, andR. A. Good: Amer. J. Path.40, 519–530 (1962).

    CAS  PubMed  PubMed Central  Google Scholar 

  60. — — —: Blood20, 118–119 (1962).

    Google Scholar 

  61. Potel, J.: Arzneimittel-Forsch.15, 527–532 (1965).

    CAS  Google Scholar 

  62. —, u.N. Brock: Arzneimittel-Forsch.15, 659–666 (1965).

    CAS  Google Scholar 

  63. Prendergast, R. A.: J. exp. Med.119, 377–387 (1964).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  64. Rebuck, J. W., andJ. H. Crowley: Ann. N.Y. Acad. Sci.59, 757–805 (1955).

    Article  CAS  PubMed  Google Scholar 

  65. Rothschild, H., J. S. Friedenwald, andC. Bernstein: Bull. Johns Hopk. Hosp.54, 232 (1934); zit. n. R. H.Follis, Jr.: Bull. Johns Hopk. Hosp.63, 283 (1938).

    Google Scholar 

  66. Schick, P., F. Trepel u.H. Begemann: Z. ges. exp. Med.148, 275–305 (1968).

    Article  CAS  Google Scholar 

  67. Schwartz, R. S.: Progr. Allergy9, 246–303 (1965).

    CAS  Google Scholar 

  68. —, andL. Beldotti: Science149, 1511–1514 (1965).

    Article  CAS  PubMed  Google Scholar 

  69. Scothorne, R. J.: J. Anat. (Lond.)90, 417–427 (1956).

    CAS  Google Scholar 

  70. Steffen, C.: Allgemeine und experimentelle Immunologie und Immunpathologie. Stuttgart: Thieme 1968.

    Google Scholar 

  71. Stender, H. S., D. Strauch, H. Winter u.W. Textor: Arzneimittel-Forsch.13, 1031–1034 (1963).

    CAS  Google Scholar 

  72. Studer, A.: Bull. schweiz. Akad. med. Wiss.8, 60–66 (1952).

    CAS  PubMed  Google Scholar 

  73. Tolderlund, K., K. Bunch-Christensen, andH. Waaler: Bull. Wld Hlth Org.22, 177–184 (1960).

    CAS  Google Scholar 

  74. Trepel, F., u.H. Begemann: Acta haemat. (Basel)36, 386–398 (1966).

    Article  CAS  Google Scholar 

  75. —,G. Gerstmair, R. Waubke, H. Pichlmaier u.H. Begemann: Haematologia1, 119–129 (1967).

    Google Scholar 

  76. —, u.J. Rastetter: Blut15, 76–82 (1967).

    Article  Google Scholar 

  77. -, P.Schick u. H.Begemann: Wirkung verschiedener Zytostatika und Antiphlogistika auf die zelluläre Entzündungsreaktion. Folia haemat. (im Druck).

  78. Turk, J. L.: Int. Arch. Allergy24, 191–200 (1964).

    Article  CAS  PubMed  Google Scholar 

  79. —,C. J. Heather, andJ. V. Diengdoh: Int. Arch. Allergy29, 278–289 (1966).

    Article  Google Scholar 

  80. Uehlinger, E., u.R. Siebenmann: Bull. schweiz. Akad. med. Wiss.8, 203–212 (1952).

    CAS  PubMed  Google Scholar 

  81. Vollmer, H.: J. Pediat.39, 22–32 (1951).

    Article  CAS  PubMed  Google Scholar 

  82. Vorlaender, K. O.: Z. ärztl. Fortbild.16, 4–10 (1967).

    Google Scholar 

  83. Waksman, B. H.: Tissue damage in the “delayed” (cellular) type of hypersensitivity. In: Mechanism of Cell and Tissue Damage Produced by Immune Reactions. II. Internat. Symposium on Immunopathology 1961, Basel-Stuttgart: Schwabe 1962, p. 146–159.

    Google Scholar 

  84. Waubke, R., G. Gerstmair, F. Trepel, H. Pichlmaier u.H. Begemann: Haematologia (Budapest)1, 45–60 (1967).

    Google Scholar 

  85. Weiler, E.: Science144, 846–849 (1964).

    Article  CAS  PubMed  Google Scholar 

  86. Wiener, J., D. Spiro, andH. O. Zunker: Amer. J. Path.47, 723–763 (1965).

    CAS  PubMed  PubMed Central  Google Scholar 

  87. Wirostko, E., andS. P. Halbert: J. exp. Med.116, 653–663 (1962).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  88. Wust, C. J., C. L. Gall, andG. D. Novelli: Science143, 1041–1043 (1964).

    Article  CAS  PubMed  Google Scholar 

  89. Zollinger, H. U., M. Enderlin u.O. Spühler: Bull. schweiz. Akad. med. Wiss.8, 162–168 (1952).

    CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. I. Medizinische Abteilung des Städtischen Krankenhauses München-Schwabing, Deutschland

    F. Trepel, P. Schick & H. Begemann

Authors
  1. F. Trepel
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. P. Schick
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. H. Begemann
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Diese Untersuchungen wurden durch die Deutsche Forschungsgemeinschaft ermöglicht.

Wir danken den Firmen Asta-Werke, Brackwede, Farbwerke Hoechst, Frankfurt, Hoffmann-La Roche, Basel, Burroughs and Wellcome, London, Lederle, München, Farbenfabriken Bayer, Leverkusen, E. Lilly, Gießen, Sandoz, Nürnberg, und E. Merck, Darmstadt, für die Bereitstellung der Substanzen.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Trepel, F., Schick, P. & Begemann, H. Morphologische und funktionelle Veränderungen im Blutzellsystem des Meerschweinchens nach Behandlung mit cytostatischen Substanzen, Prednisolon und Phenylbutazon. Z. Gesamte Exp. Med. 149, 25–43 (1969). https://doi.org/10.1007/BF02203434

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02203434

Key-words

Search

Navigation