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Clinical significance of antibodies to double stranded DNA (dsDNA) for systemic lupus erythematosus (SLE)

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Summary

In sera of patients with systemic lupus erythematosus (SLE) a wide variety of antibodies against nuclear antigens can be found, including antibodies to nucleic acids, histones and non-histone nuclear proteins. Among these, antibodies to double stranded DNA (dsDNA) appear to be mainly restricted to SLE. Yet, in daily practice one also finds patients that have antibodies to dsDNA during a long time (>5 years) but have not developed SLE. These anti-dsDNA positive non-SLE patients often fulfil several of the ARA criteria for SLE though one may not conclude that they form a specific clinical subset of SLE. It has often been tried to discriminate between clinical subsets of this heterogeneous disease by studying differences within the population of anti-dsDNA antibodies. Immunospecificity, complement-fixing ability, avidity, immunoglobulin (sub)class composition have all been the subject of different studies; yet, conclusions from these studies are often contradictory and more work will be necessary to elucidate this. The prognostic significance of anti-dsDNA levels in prospective studies has been proven valuable. A continuous increase in anti-dsDNA level correlates well with the appearance of an exacerbation of the disease. In other studies merely the amount of antibodies was found to be correlated with disease activity. Therapeutical consequences of these findings are still discutable. The role of anti-dsDNA antibodies in the pathogenesis of the disease is merely based on the above mentioned correlations and on the specificity of the antibodies to SLE. Questions regarding the etiology of SLE or the mere existence of anti-dsDNA antibodies in this kind of patients are unresolved. In conclusion: research in the last decennia to the diagnostic, prognostic, pathogenic and etiological significance of anti-dsDNA antibodies to SLE only showed clinical specificity and prognostic significance for this disease. Clinical subdivision still is only dawning.

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References

  1. Lachman, P.J., Petrie, A. Detection of anti-dsDNA as diagnostic tool. Ann Rheum Dis 1977, 36, suppl. 1, 76–81.

    Google Scholar 

  2. Aarden, L.A. Measurement of anti-dsDNA antibodies. Ann Rheum Dis 1977, suppl. 36, 91–95.

    Google Scholar 

  3. Koffler, D., Carr, R.I., Agnello, V., Fiezi, T., Kunkel, H.G., Antibodies to polynucleotides: Detection in human serum. Science 1969, 166, 1648–1649.

    Google Scholar 

  4. Kredich, N.M., Skyler, J.S., Foote, L.J. Antibodies to native DNA in systemic lupus erythematosus. Arch J Intern Med 1973, 131, 639–644.

    Google Scholar 

  5. Aarden, L.A., De Groot, E.R., Lakmaker, F., Swaak, A.J.G., Feltkamp, T.E.W.. Detection of antibodies to DNA by radioimmunoassay and immunofluorescence. Scand J Rheumatol 1975, 11, 12–19.

    Google Scholar 

  6. Fritzler, M.J., Kinsella, T.D. The crest syndrome: A distinct serologic entity with anticentromere antibodies. Am J Med 1980, 69, 520–526.

    Google Scholar 

  7. Sarai, T., Miyawaki, S., Kurata, N., Opfuji, T. Characteristics of antibody to denatured DNA in sera of patients with systemic lupus erythematosus and other rheumatic diseases. Ann J Clin Pathol 1980, 73, 761–766.

    Google Scholar 

  8. Hughes, G.R.V. Antinucleic acid antibodies in SLE: clinical and pathological significance. Clin Rheum Dis 1975, 1, 545–559.

    Google Scholar 

  9. Swaak, A.J.G., Groenwold, J., Aarden, L.A., Feltkamp, T.E.W. Detection of anti-dsDNA as diagnostic tool. Ann Rheum Dis 1981, 40, 45–49.

    Google Scholar 

  10. Lightfoot, R.W., Redecha, P.B., Levesanos, M. Longitudinal studies of anti-DNA antibody levels in SLE. Scand J Rheumatol 1975, supp. 11, 52–58.

    Google Scholar 

  11. Swaak, A.J.G., Groenwold, J., Aarden, L.A., Statius van Eps, L. W., Feltkamp, T.E.W. Prognostic value of anti-dsDNA in SLE. Ann Rheum Dis 1982, 41, 388–395.

    Google Scholar 

  12. Davis, J.S., Godfrey, S.M., Winfield, J.B. Direct evidence for circulating DNA/anti-DNA complexes in systemic lupus erythematosus. Arthritis Rheum 1978, 21, 17–22.

    Google Scholar 

  13. Koffler, D., Agnello, V., Thoburn, E., Kunkel, H.G. Systemic lupus erythematosus: prototype of immune complex nephritis in man. J Exp Med 1971, 134, 169–179.

    Google Scholar 

  14. Krishna, C., Kaplan, M.H. Immunopathologic studies of systemic lupus erythematosus. II. Antinuclear reaction of gammaglobulin eluted from homogenates and isolated glomeruli of kidneys from patients with lupus nephritis. J Clin Invest 1967, 46, 569–579.

    Google Scholar 

  15. Winfield, J.B., Koffler, D., Kunkel, H.G. Role of DNA/anti-DNA complexes in the immunopathogenesis of tissue injury in systemic lupus erythematosus. Scand J Rheumatol 1975, suppl. 11, 59–62.

    Google Scholar 

  16. Lightfoot, R.W., Hughes, G.R.V. Significance of persisting serologic abnormalities in SLE. Arthritis Rheum 1976, 19, 837–843.

    Google Scholar 

  17. Pennebaker, J.B., Gilliam, J.N., Ziff, M. Immunoglobulin classes of DNA binding activity in serum and skin in systemic lupus erythematosus. J Clin Invest 1977, 60, 1331–1338.

    Google Scholar 

  18. Ballou, S.P., Kushner, I. Immunochemical characteristics of antibodies to DNA in patients with active systemic lupus erythematosus. Clin Exp Immunol 1979, 37, 58–67.

    Google Scholar 

  19. Miniter, M.F., Stollar, B.D., Agnello, V. Reassessment of the clinical significance of native DNA antibodies in systemic lupus erythematosus. Arthritis Rheum 1979, 22, 959–968.

    Google Scholar 

  20. Aotsuka, S., Okawa, M., Ikebe, K., Yokahari, R. Measurement of anti-double stranded DNA antibodies in major immunoglobulin classes. J Immunol Methods 1979, 28, 149–162.

    Google Scholar 

  21. Feldman, M.D., Huston, D.P., Karsh, J., Balow, J.E., Klima, E., Steinberg, A.D. Correlation of serum IgG, IgM and anti native DNA antibodies with renal and clinical indexes of activity in systemic lupus erythematosus. J Rheumatol 1982, 9, 52–58.

    Google Scholar 

  22. Beaulieu, A., Quismorio, F.P., Friou, G.J., Vajavegula, B., Mirick, G. Ig antibodies to double standed DNA in systemic lupus erythematosus sera. Arthritis Rheum 1979, 22, 565–570.

    Google Scholar 

  23. Sontheimer, R.D., Gilliam, J.N. DNA antibody class, subclass and complement fixation in systemic lupus erythematosus with and without nephritis. Clin Immunol Immunopathol 1978, 10, 459–467.

    Google Scholar 

  24. Huber, O., Greenberg, M.L., Huber, J. Complement fixing anti-double stranded DNA with the Crithidia method: a better indication of active SLE than anti-DNA with the Farr-method. J Lab Clin Med 1979, 93, 32–39.

    Google Scholar 

  25. Whiteside, T.L., Dixon, J.A. Clinical usefulness of the crithidia luciliae test for antibodies to native DNA. Ann J Clin Pathol 1979, 72, 829–835.

    Google Scholar 

  26. Johnson, G.D., Edmonds, J.P., Holborow, E.J. Precipitating antibody to DNA detected by two-stage electroimmunodiffusion. Lancet 1973, 883–885.

  27. Miller, T.E., Lahita, R.G., Zarro, V.J., MacWiliam, J., Koffler, D. Clinical significance of antidouble stranded DNA antibodies detected by a solid-phase enzyme immunoassay. Arthritis Rheum 1982, 24, 602–610.

    Google Scholar 

  28. Tron, F., Bach, J.F. Relationships between antibodies to native DNA and glomerulonephritis in systemic lupus erythematosus. Clin Exp Immunol. 1977, 28, 426–432.

    Google Scholar 

  29. Winfield, J.B., Faiferman, I., Koffler, D. Avidity of anti-DNA antibodies in serum and IgG glomeruli eluted form patients with systemic lupus erythematosus. J Clin Invest 1977, 59, 90–96.

    Google Scholar 

  30. Leon, S.A., Green, A., Ehrlich, G.E., Poland, M., Shapiro, B. Avidity of antibodies in SLE: relationship to severity of renal involvement. Arthritis Rheum 1977, 20, 23–29.

    Google Scholar 

  31. Halsey, J.F., Woolery, W.A., Oleinick, S., Kahaleh, M.B., Carwile, E. DNA binding activity of serum from patients with systemic lupus erythematosus. Clin Exp Immunol 1979, 35, 356–363.

    Google Scholar 

  32. Adler, M.K., Baumgarten, A., Hecht, B., Siegel, N.J. Prognostic significance of DNA binding capacity patterns in patients with lupus nephritis. Ann Rheum Dis 1975, 34, 444–448.

    Google Scholar 

  33. Hahn, B.H., Bagby, M.K., Hamilton, T.R., Osterland, C.K. Comparison of therapeutic and immuno suppressive effects of azathioprine, prednisolone and combined therapy in NZB/NZW mice. Arthritis Rheum 1973, 16, 163–168.

    Google Scholar 

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Authors and Affiliations

  1. Department of Autoimmune Diseases, Central Laboratory of the Blood Transfusion Service, Amsterdam

    T. Swaak

  2. Department of Rheumatology, Dr. Daniël den Hoed Clinic, Rotterdam, The Netherlands

    R. Smeenk

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  1. R. Smeenk
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  2. T. Swaak
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Smeenk, R., Swaak, T. & Smeenk, R. Clinical significance of antibodies to double stranded DNA (dsDNA) for systemic lupus erythematosus (SLE). Clin Rheumatol 6 (Suppl 1), 56–63 (1987). https://doi.org/10.1007/BF02200721

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