Summary
The α and ß forms of the pregnancy-associated protein, SP1, have been studied in late pregnancy serum and in similar serum after acidification. In both sera only two forms of the protein, SP1α and SP1ß, could be found; both reacting with antisera against SP1. On gel chromatography these two forms could be separated, with the intermediate effluent containing a varying mixture of both proteins. Immunoelectrophoresis of the effluent fractions from gel chromatography showed rocket shaped immunoprecipitates whose morphology depended on the mixture of SP1α and SP1ß. Crossed immunoelectrophoresis confirmed that only two proteins could be defined with SP1 antisera; the front running protein having an α2 electrophoretic mobility and a molecular weight of 430,000, while the slower moving component had the electrophoretic mobility of a ß1 globulin and a molecular weight of 90,000.
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Ahmed AM, Toop KM, Klopper A (1981) The demonstration of two pregnancy-associated proteins with SP1 determinants in placental extracts. Placenta 2: 45–52
Ahmed AG, Klopper A (1980) Separation of two pregnancy-associated proteins with SP1 determinants and the conversion of SP1ß and SP1α. Arch Gynecol 230: 95–108
Ahmed AM, Klopper A (1981) Positive and negative affinity chromatography of SP1 Placenta (in press)
Bohn H (1971) Nachweis und Charakterisierung von Schwangerschaftsproteinen in der menschlichen Placenta sowie ihre quantitative immunologische Bestimmung im Serum schwangerer Frauen. Arch Gynäkol 210: 440–457
Bohn H (1979) Placental and pregnancy proteins. In: Lehmann F-G (ed) Carcino-embryonic proteins. Elsevier/North-Holland Biomedical Press, Amsterdam, pp 289–299
Bruce D, Klopper A (1978) The measurement of pregnancy-specific ß1 glycoprotein by electroimmunodiffusion. Clin Chim Acta 84: 107–113
Gall SA, Halbert SP (1972) Antigenic constitutents in pregnancy plasma which are undetectable in the normal non-pregnant female or male plasma. Int Arch Allergy Appl Immunol 42: 503–515
Hindersson P, Folkersen J, Westergaard JG, Teisner B, Svehag S (1979) Specific assay for pregnancy-specific ß1-glycoprotein (SP1ß). Preparation of antiserum specific for SP1ß by absorption with a crossreacting high molecular weight serum protein. J Reprod Immunol 1: 159–166
Tatarinov YS, Masyukevich VN (1970) Immunological identification of a new betaglobulin in the blood serum of pregnant women. Bull Exp Biol Med (USSR) 69: 66–68
Teisner B, Westergaard JG, Folkerser J, Husby S, Svehag S (1978) Two pregnancy-associated serum proteins with pregnancy-specific glycoprotein determinants. Am J Obstet Gynecol 131: 262–266
Westergaard JG, Teisner B, Folkersen J, Hindersson P, Schultz-Larsen P, Svehag S (1979) Characterisation of two pregnancy-associated serum proteins with pregnancy-specific ß1 glycoprotein (PSß1G)-determinants. Scand J Clin Lab Invest 39: 351–359
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Ahmed, A.G., Klopper, A. An immunochemical study of proteins with SP1 determinants in native and acidified pregnancy serum. Arch. Gynecol. 230, 283–291 (1981). https://doi.org/10.1007/BF02199676
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DOI: https://doi.org/10.1007/BF02199676