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Molecular cloning of theEnvC gene ofEscherichia coli

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Abstract

DNA fragments complementing theenvC mutation could be isolated by cloning chromosomal DNA in the vector pUH84. When the frequencies of transformation and the frequencies of restoring theenvC + phenotype were compared, the high copy number hybrid plasmids complemented with a frequency of 10−5. After subcloning theenvC-complementing DNA fragment into the low copy number plasmid pLG339, efficient complementation was achieved by spontaneous integration of the IS2 element ofEscherichia coli. By nucleotide sequence analysis, a potential promoter, a ribosome-binding site, and an unidentified reading frame were detected in the respective DNA fragment.

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Authors and Affiliations

  1. Department of Biology, Division of Microbiology, University of Kaiserslautern, Kaiserslautern, Germany

    Jürgen Robert Klein, Bernhard Henrich & Roland Plapp

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  1. Jürgen Robert Klein
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  2. Bernhard Henrich
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  3. Roland Plapp
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Robert Klein, J., Henrich, B. & Plapp, R. Molecular cloning of theEnvC gene ofEscherichia coli . Current Microbiology 21, 341–347 (1990). https://doi.org/10.1007/BF02199435

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