Neurophysiology

, Volume 20, Issue 4, pp 323–330 | Cite as

Electrophysiological research into afferent connections of embryonic neocortex allotransplants grafted into the association cortex of adult rats

  • S. V. Girman
  • I. L. Golovina
Article
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Abstract

Grafts of the rat fetal neocortex at the 17–18th day of gestation were placed in the cavity made by aspiration in the primary visual or somatosensory cortex of adult rats. Findings from electrophysiological research performed 3–3.5 months after this transplant showed that neurons of this transplant responded to sensory stimulation specific to the cortical regions replaced by the transplant in 50% of animals. This response was evoked by stimulating local receptive fields displaying a topical organization pattern in a proportion of the animals. Neuronal response in the transplant indicated that the usual field of vision previously existing on the replaced portions of visual cortex had been restored. Electrical stimulation applied locally to a number of brain structures showed that the transplants received afferent inputs from the thalamic nucleus normally projecting to the cortical region replaced by the graft, as well as from homotopic sites on the contralateral cortex. Latencies and time course of neuronal response to stimulating these regions of the host brain resemble those observed in the normal. Afferent inputs from the host brain to cortical transplants thus emulate normal cortical input. Possible mechanisms underlying reinnervation of the grafts are discussed.

Keywords

Receptive Field Cortical Region Somatosensory Cortex Thalamic Nucleus Neuronal Response 

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Literature Cited

  1. 1.
    A. G. Bragin, “Involvement of neurons of a rat fetal neocortical graft in implementing sensory function in the host brain cortex,” Zh. Vyssh. Nerv. Deyat.,36, No. 5, 930–938 (1986).Google Scholar
  2. 2.
    L. V. Polezhaev and M. A. Aleksandrova, Brain Tissue Grafts in Disease and in the Normal [in Russian], Nauka, Moscow (1986).Google Scholar
  3. 3.
    A. J. Aguayo, M. Benfy, and S. David, “A potential for axonal regeneration in neurons of the adult mammalian nervous system,” In: Nervous System Regeneration, B. Haber, et al., editors, New York (1983), pp. 327–340.Google Scholar
  4. 4.
    M. Benfy, V. Buenger, M. Vidal-Sanz, et al., “Axonal regeneration from GABA-ergic neurons in the adult rat thalamus,” J. Neurocytol.,14, No. 2, 279–296 (1985).Google Scholar
  5. 5.
    A. Björklund and U. Stenevi, “Intracerebral neural transplants: neuronal replacements and reconstruction of damaged circuitries,” Ann. Rev. Neurosci.,7, 279–308 (1984).Google Scholar
  6. 6.
    F.-L. F. Chang, J. G. Steedman, and R. D. Lund, “The lamination and connectivity of embryonic cerebral cortex transplanted into newborn rat cortex,” J. Comp. Neurol.,244, No. 3, 401–411 (1986).Google Scholar
  7. 7.
    J. R. R. Konig and R. A. Knippel, The Rat Brain, R. E. Krieger, New York (1970).Google Scholar
  8. 8.
    L. F. Kromer, A. Björklund, and U. Stenevi, “Innervation of embryonic hippocampal implants by regenerating axons of cholinergic septal neurons in the adult rat,” Brain Res.,210, No. 1/2, 153–171 (1981).Google Scholar
  9. 9.
    L. F. Kromer, A. Björklund, and U. Stenevi, “Regeneration of the septohippocampal pathways in adult rats is promoted by utilizing embryonic hippocampal implants as bridges,” Brain Res.,210, No. 1/2, 173–200 (1981).Google Scholar
  10. 10.
    R. D. Lund, M. H. Hankin, V. H. Perry, et al., “Retinae transplanted to rat brains,” In: Retinal Signal Systems, Degenerations and Transplants, E. Agardh and B. Ehinger, editors, New York (1986), pp. 243–255.Google Scholar
  11. 11.
    K. F. So and A. J. Aguayo, “Lengthy regrowth of cut axons from ganglion cells after peripheral nerve transplantation into the retina of adult rats,” Brain Res.,328, No. 2, 349–354 (1985).Google Scholar
  12. 12.
    O. S. Vinogradova, A. G. Bragin, and V. F. Kitchigina, “Spontaneous and evoked activity of neurons in intrabrain allo- and xenografts of the hippocampus and septum,” in: Neural Grafting in the Mammalian CNS, A. Björklund and U. Stenevi, editors, Amsterdam (1985), pp. 409–420.Google Scholar

Copyright information

© Plenum Publishing Corporation 1989

Authors and Affiliations

  • S. V. Girman
  • I. L. Golovina

There are no affiliations available

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