Summary
The seasonal and diurnal variations in nitrate reductase (NR) activity of field grown ‘Altona’ soybean, with and without applied nitrogen, were determined. The NR activity in the fortnightly collected leaf samples was, on the average, 20 percent higher throughout the season in N-treated plants, the highest being early in the season and declining gradually in the samples of subsequent dates. Diurnal variations were marked by increase in the NR activity from 7 a.m. to 7 p.m. and then declining gradually to a minimum at 7 a.m. the next morning.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Afridi, M. M. R. K. and Hewitt, E. J. 1964 The inducible formation and stability of nitrate reductase in higher plants. 1. Effects of nitrate and molybdenum on enzyme activity in cauliflower (Brassic oleracea var. botrytis). J. Exp. Bot.15, 251–271.
Beevers, L. and Hageman, R. H. 1969 Nitrate reductase in higher plants. Annu. Rev. Plant Physiol.20, 495–522.
Chantarotwong, W., Huffaker, R. C., Miller, B. L. and Granstedt, R. C. 1976In vivo nitrate reduction in relation to nitrate uptake, content andin vitro nitrate reductase activity in intact barley seedlings. Plant Physiol.57, 519–522.
Chen, T. M. and Reis, S. K. 1969 Effect of light and temperature on nitrate uptake and nitrate reductase activity in rye and oat seedlings. Can. J. Bot.47, 341–343.
Hageman, R. H. and Flesher, Donna 1969 Nitrate reductase activity in corn seedlings as affected by light and nitrate content of nutrient media. Plant Physiol.35, 700–708.
Harper, J. E. and Cooper, R. L. 1971 Nodulation response of soybeans to application, rate and placement of combined nitrogen. Crop Sci.11, 438–440.
Harper, J. E. and Hageman, R. H. 1972 Canopy and seasonal profiles of nitrate reductase in soybean (Glycine max (L) Merr). Plant Physiol.49, 146–154.
Harper, J. E., Nicholas, J. S. and Hageman, R. H. 1972 Seasonal and canopy variation in nitrate reductase activity of soybean (Clycine max (L) Merr) varieties. Crop Sci.12, 382–386.
Hatam, M. 1977 Effect of various sources of nitrogen and added organic matter on nodulation, nodule activity and nitrogen content in soybean. J. Sci. Techn.1, 31–35.
Hatam, M. 1978 Comparison ofin vivo andin vitro assays for nitrate reductase activity determination in soybean tissues. J. Soil Sci. Plant Nutr.24, 315–318.
Jawaraski, E. G. 1971 Nitrate reductase assay in intact plant tissues. Biochem. Biophy. Res. Commun.43, 1274–1279.
Liu, M. C. and Hadley, H. H. 1971 Relationship of nitrate reductase activity to protein content in related nodulating and non-nodulating soybeans. Crop Sci.11, 467–471.
Neyra, C. A. and Hageman, R. H. 1975 Nitrate intake and induction of nitrate reductase in excised corn roots. Plant Physiol.56, 692–695.
Streeter, J. G. 1972 Nitrogen nutrition of field grown soybean plants II. Seasonal variations in nitrate reductase, glutamate dehydrogenase and nitrogen constituents of plant parts. Agron. J.64, 315–319.
Wallace, W. and Pate, J. S. 1965 Nitrate reductase in the field pea (Pisum arvense L.). Ann. Bot.29, 655–671.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hatam, M. Seasonal and diurnal variations in nitrate reductase activity of soybean (Glycine max (L.) Merr.). Plant Soil 56, 27–32 (1980). https://doi.org/10.1007/BF02197949
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF02197949