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Plant and Soil

, Volume 73, Issue 1, pp 27–34 | Cite as

Influence of humic acid on iron required for the formation of hydroxyproline in discs ofBeta vulgaris L. storage tissue

  • D. Vaughan
  • B. G. Ord
Article
  • 38 Downloads

Summary

When discs of beetroot storage tissue are washed (aged) in water for three days, there is at least a 100 per cent increase in the content of their cell-wall bound hydroxyproline. Humic acid, prepared from an agricultural soil, enhances this increase. The effects of humic acid on the increase in the hydroxyproline content of cell walls, as well as on the formation of14C-labelled hydroxyproline from14C-proline, are compared with those effects produced by the chelating agents 2,2′-dipyridyl and 8-hydroxyquinoline. It is suggested that humic acid enhances hydroxyproline formation either by a mechanism which renders more ferrous iron available for the hydroxylation of proline to hydroxyproline within the tissue, and/or a mechanism which stimulates the enzyme required for the hydroxylation process.

Key words

Beta vulgaris Cell walls Chelating agents Humic acid Hydroxyproline Iron Proline Proteins Storage tissue 

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Copyright information

© Martinus Nijhoff/Dr W. Junk Publishers 1983

Authors and Affiliations

  • D. Vaughan
    • 1
  • B. G. Ord
    • 1
  1. 1.The Macaulay Institute for Soil ResearchAberdeenUK

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