Plant and Soil

, Volume 88, Issue 3, pp 333–343 | Cite as

The response of field grownPhaseolus vulgaris to Rhizobium inoculation and the quantification of N2 fixation using15N

  • F. F. Duque
  • M. C. P. Neves
  • A. A. Franco
  • R. L. Victoria
  • R. M. Boddey


A field experiment was performed to assess the effects of Rhizobium inoculation and nitrogen fertilizer (100 kg N ha−1) on four cultivars of Phaseolus beans; Carioca, Negro Argel, Venezuela 350 and Rio Tibagi. In the inoculated treatment 2.5 kg N ha−1 of15N labelled fertilizer was added in order to apply the isotope dilution technique to quantify the contribution of N2 fixation to the nutrition of these cultivars.

Nodulation of all cultivars in the uninoculated treatments was poor, but the cultivars Carioca and Negro Argel were well nodulated when inoculated. Even when inoculated, nodulation of the cultivars Venezuela 350 and Rio Tibagi was poor and these cultivars showed little response to inoculation in terms of nitrogen accumulation or grain yield. The estimates of the contribution of N2 fixation estimated using the isotope dilution technique, for the Carioca and Negro Argel cultivars, amounted to 31.7 and 18.4 kg N ha−1 respectively. These two cultivars produced 991 and 883 kg ha−1 of grain, respectively, when inoculated and 663 and 620 kg ha−1 with the addition of 100 kg N ha−1 of N fertilizer. The response to nitrogen was particularly poor due to high leaching losses in the very sandy soil at the experimental site.

The Venezuela 350 and Rio Tibagi cultivars only responded to N fertilizer and not to inoculation with Rhizobium which stresses the great importance of selecting plant cultivars for nitrogen fixation in the field.

Key words

Acetylene reduction Bean cultivars Harvest index 15N dilution Nitrogen fixation Nodulation Phaseolus vulgaris Rhizobium inoculation 


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Copyright information

© Martinus Nijhoff Publishers 1985

Authors and Affiliations

  • F. F. Duque
    • 1
  • M. C. P. Neves
    • 1
  • A. A. Franco
    • 1
  • R. L. Victoria
    • 1
  • R. M. Boddey
    • 1
  1. 1.EMBRAPA-Programa Nacional de Pesquisa em Biologia do SoloRio de JaneiroBrazil

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