Abstract
Sections of the tibias from intact and from aparathyroid animals were examined histologically and histochemically to determine the changes in bone following parathyroidectomy. The proximal epiphyseal cartilages of parathyroidectomized rats showed arelative increase of intercellular matrix, decrease in cellularity, and disorganization of the cellular arrangement. The epiphyseal cartilages of the aparathyroid rats exhibited an intense PAS-positive intercellular matrix in contrast to the alcianophilic nature of the matrix from control animals. The diaphyseal trabeculae from aparathyroid animals stained red with PAS and contained wide regions of calcified cartilaginous cores. In addition, the epiphyseal cartilages and bone trabeculae from the parathyroidectomized animals stained less intensely to the ninhydrin-Schiff reaction than did the bone from the intact animal. The response to the various histochemical reactions indicated that following parathyroidectomy there was a change in the collagen-protein mucopolysaccharide components. This condition would produce an alteration in the calcification of bone.
Résumé
Des coupes de tibias d'animaux entiers et parathyroidectomisés sont étudiées histologiquement et histochimiquement pour préciser les effets de la parathyroidectomie sur l'os. Les épiphyses cartilagineuses proximales montrent uneaugmentation relative de matrice intercellulaire, une diminution en cellules, et une désorganisation de l'arrangement cellulaire. Le cartilage épiphysaire des rats parathyroidectomisés montre une matrice intercellulaire intensément PAS positive, contrairement aux affinités à l'alcian de la matrice des animaux témoins. Les travées diaphysaires des animaux parathyroidectomisés se colorent en rouge au PAS et contiennent de nombreux ilôts étendus de cartilage calcifié. En outre, le cartilage épiphysaire et les travées osseuses des animaux parathyroidectomisés se colorent moins intensément à la réaction de ninhydrine—Schiff que l'os des témoins. L'étude par les diverses colorations histochimiques, après parathyroidectomie, indique une altération des constituants collagène-protéine et mucopolysaccharide. Ces changements sont susceptibles de produire un trouble de la calcification de l'os.
Zusammenfassung
Tibiaschnitte von Kontrollratten und von parathyreoidektomierten Ratten wurden histologisch und histochemisch untersucht, um die nach Parathyreoidektomie aufgetretenen Knochenveränderungen festzustellen. Der proximale Epiphysenknorpel von parathyreoidektomierten Ratten zeigte einerelative Zunahme der intracellulären Matrix, eine Abnahme des Zellgehaltes und eine Veränderung der zellulären Anordnung. Die Epiphysenknorpel der parathyeoidektomierten Ratten wiesen eine intensiv PAS-positive intracelluläre Matrix auf, im Gegensatz zur alcianophilen Reaktion der Matrix der Kontrolltiere. Die Diaphysen-Trabekel der parathyreoidektomierten Tiere ließen sich mit PAS rot färben und enthielten breite Zonen von verkalkten knorpeligen Kernen. Ferner ließen sich die Epiphysenknorpel und die Knochen-Trabekel von parathyreoidektomierten Tieren im Vergleich mit den Kontrolltieren weniger intensiv mit der Ninhydrin-Schiff-Reaktion färben. Die Resultate der verschiedenen histochemischen Reaktionen zeigten, daß die Parathyreoidektomie eine Veränderung der Protein-Mucopolysaccharide des Kollagens zur Folge hat. Diese neuen Bedingungen könnten eine Veränderung der Knochenverkalkung hervorrufen.
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This investigation was supported by USPH Research Grant HD-00185-06 from the National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland.
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Bernick, S. Histochemical study of bone in parathyroidectomized rats. Calc. Tis Res. 6, 316–328 (1970). https://doi.org/10.1007/BF02196212
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DOI: https://doi.org/10.1007/BF02196212