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Lipid metabolism of chick epiphyseal bone and cartilage

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Abstract

The content and composition of lipids of chick epiphyseal tissue were investigated. The amounts of total lipids, triglycerides and phospholipids in bone were 7.50, 7.81 and 1.02 mg/g, respectively, and in cartilage, 5.20, 1.46 and 0.53 mg/g, respectively. The main fatty acids of both bone and cartilage were palmitic and oleic in phospholipids, and palmitic, oleic and linoleic in triglycerides. For the substrates studied, the order of incorporation into total lipids of both bone and cartilage was palmitate > glucose > acetate > citrate. Acetate was the main precursor for iatty acid synthesis whereas only a minor portion of glucose was found in the fatty acids. Esterification appeared to be the predominant pathway of lipid synthesis in chick bone and cartilage.

Résumé

Le contenu et la composition des lipides du tissue épiphysaire ont été étudiés chez le poulet. Les valeurs des lipides totaux, des triglycérides et des phospholipides sont, respectivement dans l'os, de 7,50, 1,81 et 1,02 mg/g et, dans le cartilage, de 5,20, 1,46 et 0,53 mg/g. Les principaux acides gras trouvés dans l'os aussi bien que dans le cartilage sont les acides palmitique et oléique, parmi les phospholipides, et les acides palmitique, oléique et linoléique parmi les triglycérides. Quant aux substrats étudiés, l'ordre d'incorporation dans les lipides totaux de l'os et du cartilage a été: palmitate > glucose > acétate > citrate. L'acétate était le principal précurseur dans la synthèse des acides gras, alors que seule une faible protion de glucose a été trouvée dans les acides gras. L'estérification semble donc être la voie prédominante de la synthèse des lipides dans l'os et le cartilage du poulet.

Zusammenfassung

Es wurde eine Untersuchung über den Gehalt und die Zusammensetzung der Lipide im Epiphysengewebe von Hühnchen durchgeführt. Es wurden 7,50 mg Gesamtlipide, 1,81 mg Triglyceride und 1,02 mg Phospholipide per Gramm Knochen gefunden. Die entsprechenden Werte per Gramm Knorpel waren 5,20, 1,46 und 0,53 mg. Die hauptsächlichsten Fettsäuren in den Phospholipiden von Knochen- und Knorpelgewebe waren Palmitin- und Ölsäure, in den Triglyceriden Palmitin-, Öl- und Linolsäure. Die untersuchten Verbindungen wurden in nachstehender Reihenfolge in Knochen- und Knorpel-Lipide eingebaut: Palmitin > Glucose > Acetat > Citrat. Acetat war die hauptsächliche Ausgangssubstanz für die Fettsäure-Synthese, während nur ein unbeträchtlicher Teil der Glucose in den Fettsäuren vorgefunden wurde. Veresterung ist anscheinend der vorherrschende Weg der Fettsynthese in Knorpel- und Knochengewebe von Hühnchen.

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Authors and Affiliations

  1. Department of Nutrition, Hebrew University-Hadassah Medical School, Jerusalem, Israel

    I. Wolinsky & K. Guggenheim

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  1. I. Wolinsky
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  2. K. Guggenheim
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Wolinsky, I., Guggenheim, K. Lipid metabolism of chick epiphyseal bone and cartilage. Calc. Tis Res. 6, 113–119 (1970). https://doi.org/10.1007/BF02196190

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