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Journal of Biomolecular NMR

, Volume 2, Issue 6, pp 631–637 | Cite as

Improved 4D NMR experiments for the assignment of backbone nuclei in13C/15N labelled proteins

  • Wayne Boucher
  • Ernest D. Laue
  • Sharon L. Campbell-Burk
  • Peter J. Domaille
Article

Summary

We recently proposed a novel 4D NMR strategy for the assignment of backbone nuclei in13C/15N-labelled proteins (Boucher et al., 1992). Intra-residue (and many sequential) assignments are obtained from a HCANNH experiment, whereas sequential assignments are based on a complementary HCA(CO)NNH experiment. We present here new constant time 4D HCANNH, HCA(CO)NNH and HNCAHA experiments that are more sensitive. Some of the data were presented at the 33rd ENC held at Asilomar, California, U.S.A., in April 1992.

Keywords

4D NMR Resonance assignment Backbone nucle Larger proteins Isotopic labelling c-H-ras p21 

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Copyright information

© ESCOM Science Publishers B.V. 1992

Authors and Affiliations

  • Wayne Boucher
    • 1
  • Ernest D. Laue
    • 1
  • Sharon L. Campbell-Burk
    • 2
  • Peter J. Domaille
    • 2
  1. 1.Cambridge Centre jor Molecular Recognition, Department of BiochemistryUniversity of CambridgeCambridgeUK
  2. 2.DuPont Merck Pharmaceutical CoWilmingtonUSA

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