Journal of Biomolecular NMR

, Volume 2, Issue 6, pp 619–629 | Cite as

Processing of multi-dimensional NMR data with the new software PROSA

  • Peter Güntert
  • Volker Dötsch
  • Gerhard Wider
  • Kurt Wüthrich


The new program PROSA is an efficient implementation of the common data-processing steps for multi-dimensional NMR spectra. PROSA performs linear prediction, digital filtering, Fourier transformation, automatic phase correction, and baseline correction. High efficiency is achieved by avoiding disk storage of intermediate data and by the absence of any graphics display, which enables calculation in the batch mode and facilitates porting PROSA on a variety of different computer systems; including supercomputers. Furthermore, all time-consuming routines are completely vectorized. The elimination of a graphics display was made possible by the use of a new, reliable automatic phase-correction routine. CPU times for complete processing of a typical heteronuclear three-dimensional NMR data set of a protein vary between less than 1 min on a NEC SX3 supercomputer and 40 min on a Sun-4 computer system.


Data processing Fourier transformation Linear prediction Phase correction Baseline correction 











nuclear Overhauser enhancement spectroscopy


total correlation spectroscopy


time-proportional phase incrementation




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Copyright information

© ESCOM Science Publishers B.V. 1992

Authors and Affiliations

  • Peter Güntert
    • 1
  • Volker Dötsch
    • 1
  • Gerhard Wider
    • 1
  • Kurt Wüthrich
    • 1
  1. 1.Eidgenössische Technische Hochschule-HönggerbergInstitut für Molekularbiologie und BiophysikZürichSwitzerland

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