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Caffeine-resistance in fission yeast is caused by mutations in a single essential gene,crm1 +

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Abstract

Caffeine is a base analogue and is known to affect a wide variety of cellular processes. In order to dissect genetically molecules which mediate the biological effects of caffeine, temperature-sensitive (ts) and caffeine-resistant mutants were isolated from fission yeast,Schizosaccharomyces pombe. Surprisingly, all twelve is isolates contained a mutation in the same locus,crm1. Cells of the iscrm1 mutant showed an abnormal chromosome structure at the restrictive temperature, an elevated expression of Pap1-dependent transcription, and cross-resistance to an unrelated drug such as staurosporine. Overproduction ofpap1 + also conferred caffeine resistance, whilst the resistance of thecrm1 mutant is abolished in thepap1 − background. These results show that thecrm1 + gene is a major locus for caffeine resistance, which arises from Pap1-dependent transcriptional activation.

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Authors and Affiliations

  1. Department of Biophysics, Faculty of Science, Kyoto University, Kitashirakawa Oiwake-cho, 606-01, Sakyo-ku, Kyoto, Japan

    Kazuki Kumada & Mitsuhiro Yanagida

  2. Laboratory of Cell Regulation, Imperial Cancer Research Fund, 44 Lincoln's Fields, P.O. Box 123, WC2 3PX, London, UK

    Takashi Toda

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  1. Kazuki Kumada
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  2. Mitsuhiro Yanagida
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  3. Takashi Toda
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Communicated by M. Sekiguchi

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Kumada, K., Yanagida, M. & Toda, T. Caffeine-resistance in fission yeast is caused by mutations in a single essential gene,crm1 + . Molec. Gen. Genet. 250, 59–68 (1996). https://doi.org/10.1007/BF02191825

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  • DOI: https://doi.org/10.1007/BF02191825

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