Molecular and General Genetics MGG

, Volume 248, Issue 6, pp 755–766 | Cite as

Theraspberry locus encodesDrosophila inosine monophosphate dehydrogenase

  • Roger Slee
  • Mary Bownes
Original Paper

Abstract

Investigation of an enhancer-trap line exhibiting testis-specificβ-galactosidase expression led to the isolation of theDrosophila gene encoding inosine monophosphate dehydrogenase (IMPD), the rate-limiting enzyme in guanine nucleotide synthesis, which has been implicated in cell cycle control and malignant transformation. Northern and in situ hybridization analysis demonstrated that the gene has a complex expression pattern involving several independently regulated transcripts. Two ubiquitous, but highly ovary enriched, transcripts of 2.5 and 1.9 kb are expressed in the nurse cells and delivered to the oocyte, whilst a 0.9 kb transcript is found exclusively in the testis. The 2.5 kb transcript encodes a 58 kDa protein, which is highly similar in length and sequence to mouse and human IMPDs and is presumably required for GTP synthesis during early embryogenesis. Over-expression of this cDNA inEscherichia coli yielded a product of the predicted size, which was demonstrated to possess IMPD activity in a spectrophotometric assay. The coding capacity of the other transcripts is currently uncertain. We present evidence that IMPD is the product of theraspberry (ras) locus at 9E and the functions of the gene are discussed in relation to the phenotypes ofras mutants.

Key words

Drosophila Inosine monophosphate dehydrogenase Guanine nucleotide metabolism 

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Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • Roger Slee
    • 1
  • Mary Bownes
    • 1
  1. 1.Institute of Cell and Molecular BiologyUniversity of EdinburghEdinburghUK

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