Molecular and General Genetics MGG

, Volume 248, Issue 4, pp 423–433 | Cite as

Characterization ofGandalf, a new inverted-repeat transposable element ofDrosophila koepferae

  • Ignacio Marín
  • Antonio Fontdevila
Original paper


The cloning and characterization ofGandalf, a new DNA-transposing mobile element obtained from theDrosophila koepferae (repleta group) genome is described. A fragment ofGandalf was found in a middle repetitive clone that shows variable chromosomal localization. Restriction, Southern blot, PCR and sequencing analyses have shown that mostGandalf copies are about 1 kb long, are flanked by 12 by inverted terminal repeats and contain subterminal repetitive regions on both sides of the element. As with other elements of the DNA-transposing type (known as the ‘Ac family’), theGandalf element generates 8 by direct duplications at the insertion point. Coding region analysis has shown that the longer open reading frame found inGandalf copies could encode part of a protein. However, whether or not the 1 kb copies of the element are actually the active transposons remains to be elucidated.Gandalf shows a very low copy number inD. buzzatii, a sibling species ofD. koepferae. An attempt to induce interspecific hybrid dysgenesis in hybrids of these two species has been unsuccessful.

Key words

Drosophila Transposable elements repleta group Ac family Hybrid instability 


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Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • Ignacio Marín
    • 1
  • Antonio Fontdevila
    • 1
  1. 1.Departamento de Genética y MicrobiologiaUniversidad Autónoma de BarcelonaBarcelonaSpain

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