Summary
Human lymphocytes are widely used as peripheral models for central neurones. Alterations in immune function have been reported in depressed patients, e.g. mitogen-induced proliferation is impaired during depression. One possible causative mechanism could be altered [Ca2+]i regulation. Phytohaemagglutinin (PHA)-induced rise of [Ca2+]i has been found to be diminished in lymphocyte suspensions from depressed patients (Ecker et al., this issue). We measured PHA-induced rise of [Ca2+]i in single Fura-2 AM-loaded T11+ lymphocytes of patients with major depression and controls to further analyse [Ca2+]i regulation in depression.
The [Ca2+]i of resting lymphocytes was 57±2 nmol/l (mean ± SEM). There was no difference in resting [Ca2+]i of resting lymphocytes of patients and controls. PHA evoked an increase of [Ca2+]i an 7 out of 14 cells from control subjects up to 400–500 nmol/l. In contrast, only 4 out of 13 cells from depressed patients showed an increase of [Ca2+]i up to 200 nmol/l. In a small fraction of cells from both groups the [Ca2+]i signal is oscillating.
Our preliminary data confirm alteration of [Ca2+]i regulation in lymphocytes of depressed patients.
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Vollmayr, B., Aldenhoff, J.B. Cytosolic free [Ca2+] in single T-lymphocytes from depressed patients and healthy controls. Eur Arch Psychiatry Clin Nuerosci 243, 214–217 (1994). https://doi.org/10.1007/BF02191576
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DOI: https://doi.org/10.1007/BF02191576