Abstract
Ribonuclease II (encoded byrnb) is one of the two main exonucleases involved in mRNA degradation inEscherichia coli. We report the precise physical mapping ofrnb to 29 min on the chromosomal map in the vicinity ofpyrF, and clarify the genetic and physical maps of thisE. coli chromosomal region. The results were confirmed by the construction of a strain partially deleted forrnb.
This is a preview of subscription content, log in via an institution to check access.
References
Alix JH (1989) A rapid procedure for cloning genes fromλ libraries by complementation ofE. coli defective mutants: application to thefabE region of theE. coli chromosome. DNA 8: 779–789
Arraiano CM, Yancey SD, Kushner SR (1988) Stabilization of discrete mRNA breakdown products inams pnp rnb multiple mutants ofEscherichia coli K-12. J Bacteriol 170: 4625–4633
Bachmann BJ, Low KB (1980) Linkage map ofEscherichia coli K-12, edition 6. Microbiol Rev 44: 1–56
Bachmann BJ (1990) Linkage map ofEscherichia coli K-12, edition 8. Microbiol Rev 54: 130–197
Becker S, Plapp R (1992) Location of thedcp gene on the physical map ofEscherichia coli. J Bacteriol 174: 1698–1699
Dessen P, Fondrat C, Valencien C, Mugnier C (1990) BISANCE: a French service for access to molecular sequence databases. CABIOS 6: 355–356
Donovan WP, Kushner SR (1983a) Cloning and physical analysis of thepyrF gene (coding for orotidine-5′-phosphate decarboxylase) fromEscherichia coli K-12. Gene 25: 39–48
Donovan WP, Kushner SR (1983b) Amplification of ribonuclease II (rnb) activity inEscherichia coli K-12. Nucleic Acids Res 11: 265–275
Donovan WP, Kushner SR (1986) Polynucleotide phosphorylase and ribonuclease II are required for cell viability and mRNA turnover inEscherichia coli K-12. Proc Natl Acad Sci USA 83: 120–124
Henrich B, Becker S, Schroeder U, Plapp R (1993)dcp gene ofEscherichia coli: cloning, sequencing, transcript mapping and characterization of the gene product. J Bacteriol 175: 7290–7300
Jasin H, Schimmel P (1984) Deletion of an essential gene inEscherichia coli by site-specific recombination with linear DNA fragments. J Bacteriol 159: 783–786
Kohara Y, Akiyama K, Isono K (1987) The physical map of the wholeEscherichia coli chromosome: applications of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50: 495–508
Kushner SR, Nagaishi H, Templin A, Clark AJ (1971) Genetic recombination inE. coli: the role of exonuclease I. Proc Natl Acad Sci USA 68: 824–827
Ono M, Kuwano M, Horiuchi T (1977) Genetic analysis of mutations affecting ribonuclease II inEscherichia coli. Mol Gen Genet 153: 1–4
Rudd KE (1992) Alignment ofE. coli DNA sequences to a revised, integrated genomic restriction map. In: Miller JH (ed) A short course in bacterial genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual (2nd edn) Cold Spring Harbor Laboratory Press, Cold Spring harbor, New York
Zilhão R, Camelo L, Arraiano CM (1993) Sequencing and expression of the gene rnb encodingEscherichia coli ribonuclease II. Mol Microbiol 8: 43–51
Author information
Authors and Affiliations
Additional information
Communicated by K. Isono
Rights and permissions
About this article
Cite this article
Zilhão, R., Caillet, J., Régnier, P. et al. Precise physical mapping of theEscherichia coli rnb gene, encoding ribonuclease II. Molec. Gen. Genet. 248, 242–246 (1995). https://doi.org/10.1007/BF02190807
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02190807