Abstract
The activity of the yeast activator HAP1in vivo requires heme. A heme responsive domain of HAP1 was identified previously. It is adjacent to the DNA-binding domain, and appears to block DNA binding in the absence of heme. Here we describe a novel genetic selection which yielded mutants of HAP1 that are independent of heme when assayed for activation. These mutants define a second region of HAP1, close to the activation domain, which also controls its response to heme.
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Communicated by C.P. Hollenberg
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Haldi, M.L., Guarente, L. Multiple domains mediate heme control of the yeast activator HAP1. Molec. Gen. Genet. 248, 229–235 (1995). https://doi.org/10.1007/BF02190805
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DOI: https://doi.org/10.1007/BF02190805