Summary
Isoproterenol (2 mg/kg) injected subcutaneously into male Sprague-Dawley rats elicited morphological damage in the endomyocardium which was analyzed 16h following injection. Our aim was to study the relationship between damaged individual myocytes asnd their capillary supply. Myocardial tissue sections were differentially stained in order to distinguish arteriolar (AC) and venular (VC) capillary portions. Tissue areas surrounding individual capillaries and the position of the capillaries with respect to the damaged individual myocytes were established by using the method of “capillary domains”. In multicellular necrotic lesions 84 % of the capillaries located within the necrotic foci and 77 % of the capillaries in the surrounding tissue area were identified as the distal, venular portion with presumably low OZ content. The proportion of VCs related to necrotic lesions was significantly higher than in surviving endomyocardial regions. In the case of individual necrotic myocytes, we found 88 % to be supplied by VCs, while the adjacent normal myocytes were supplied by 61 % VCs. Both values were significantly higher when compared to control hearts (42 %). These results strongly support the crucial role of a lack of oxygen delivery in the pathogenesis of isoproterenol-induced necrosis.
Similar content being viewed by others
References
Batra S, Kuo C, Rakusan K (1989) Oxygen transport to tissue XI. Plenum Publishing Corporation, New York London, pp 241–247
Batra S, Rakusan K, Campbell SE (1991) Geometry of capillary networks in hypertrophied rat heart. Microvasc Res 41:29–40
Benjamin IJ, Jalil JE, Tan LB, Cho K, Weber KT, Clark WA (1989) Isoproterenol-induced myocardial fibrosis in relation to myocyte necrosis. Cite Res 65:657–670
Boutet M, Huttner I, Rona G (1976) Permeability alteration of sarcolemmal membrane in catecholamine-induced cardiac muscle cell injury. In vivo studies with fine structural diffusion tracer horseradish peroxidase. Lab Invest 34:482–488
Brown MD, Egginton S (1988) Capillary density and fine structure in rabbit papillary muscles after high dose of norepinephrine. Microvasc Res 36:1–12
Chappel CI, Rona G, Balazs T, Gaudry R (1959) Comparison of cardiotoxie actions of certain sympathomimetic amines. Canad J Biochem Physiol 37:35–42
Cimini CM, Weis HR (1990) Isoproterenol and myocardial O2 supply/consumption in hypertension-induced myocardial hypertrophy. Am J Physiol 259 (Heart Cite Physiol 28):H346-H351
Dhalla NS, Yates JC, Lee SL, Singh A (1978) Functional and subcellular changes in the isolated rat heart perfused with oxidized isoproterenol. J Mol Cell Cardiol 10:31–34
Downey JM, Kirk ES (1974) Distribution of the coronary blood flow across the canine heart wall during systole. Circ Res 34:251–257
Feigl EO (1983) Coronary physiology. Physiol Rev 63:1–205
Fleckenstein A (1983) Calcium antagonism in heart and smooth muscle. Experimental facts and therapeutic prospects. J Wiley & Sons, New York Chichester Brisbane Toronto Singapore, pp 109–120
Graham TP, Cowell JW, Sonnenblick EH, Ross J, Braunwald E (1968) Control of myocardial oxygen consumption: Relative influence of contractile state and tension development. J Clin Invest 47:375–385
Hoffman JIE, Buckberg GD (1978) The myocardial supply: Demand ratio — a critical review. Am J Cardiol 41:327–332
Jennings RB, Steenbergen C, Kinney RB, Hill ML, Reimer KA (1983) Comparison of the effect of ischemia and anoxia on the sarcolemma of the dog heart. Eur Heart J 4(H):123–137
Klocke FJ, Ellis AK (1980) Control of coronary blood flow. Annu Rev Med 31:489–508
Klocke FJ, Kaiser GA, Ross J, Braunwald E (1965) Mechanism of increase of myocardial oxygen uptake produced by catecholamines. Am J Physiol 209:913–919
Lojda Z (1979) Studies on dipeptidyl(amino)peptidase IV (glycil-proline naphthylamidase. Histochemistry 59:153–166
Reimer KA, Jennings RB (1986) Biologic basis for limitation of infarct size. Adv Exp Med Biol 194:315–330 83:567–575
Rona G, Chappel CI, Balazs T, Gaudry R (1959) An infarct-like myocardial lesion and other toxic manifestations produced by isoproterenol in the rat. AMA Arch Pathol 67:99–111
Singal PK, Dhillon DS, Beamish RE, Kapur N, Dhalla NS (1982) Myocardial cell damage and cardiovascular changes due to i.v. infusion of adrenochrome in rats. Br J Exp Pathol 63:167–176
Takatsu N, Hashimoto H, Miyazaki Y, Ito T, Oqawa K, Satake (1988) Effects of phospholipase inhibitors and calcium antagonists on the changes in myocardial phospholipids induced by isoproterenol. Basic Res Cardiol 83:567–575
Weis HR (1983) Role of beta adrenergic receptors in the control of heterogeneity of O2 saturation in small myocardial veins. J Pharamacol Exp Ther 227:333–339
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Veprek, P., Batra, S., Korecky, B. et al. Isoproterenol induced rat endomyocardial damage in relationship to local capillary geometry. Basic Res Cardiol 86, 434–440 (1991). https://doi.org/10.1007/BF02190711
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02190711