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Basic Research in Cardiology

, Volume 86, Issue 3, pp 266–272 | Cite as

Is malondialdehyde a marker of the effect of oxygen free radicals in rat heart tissue?

  • György Ballagi-Pordány
  • J. Richter
  • M. Koltai
  • Z. Aranyi
  • G. Pogátsa
  • W. Schaper
Original Contributions

Abstract

We tested the effect of exogenous purine derived free radicals and H2O2 VS ischemia and reperfusion on the thiobarbituric-acid (TBA)-reactive material and malondialdehyde (MDA) formation in isolated rat hearts using the thiobarbituric acid test and high performance lipid chromatography (HPLC). We could not detect increased thiobarbituric-acid-reactive material or MDA- production during 6 MM H2O2 infusion, during free radical generation by purine-derived free radicals, or using ischemia and reperfusion. Increased thiobarbituric-acid-reactive material and MDA tissue levels were detected only during infusion of 12 mM H2O2 (p<0.001). We conclude that the generally used thiobarbituric acid assay for MDA is susceptible to artifacts and unsuited as an indirect measure for low-to-medium-levels of oxygen free radicals. Using HPLC assay, which accurately measures MDA, no evidence was found that MDA is a primary and direct lipid peroxidation product of exogenous or endogenous reactive oxygen species.

Key words

Malondialdehyde thiobarbituric-acid-reactive material reactiveoxygen species isolatedrat heart reperfusion 

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Copyright information

© Steinkopff Verlag 1991

Authors and Affiliations

  • György Ballagi-Pordány
    • 1
  • J. Richter
    • 2
  • M. Koltai
    • 1
  • Z. Aranyi
    • 1
  • G. Pogátsa
    • 1
  • W. Schaper
    • 2
  1. 1.National Institute of CardiologyResearch DepartmentBudapestHungary
  2. 2.Max Planck InstituteDepartment of Experimental CardiologyBad NauheimGermany

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