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Radioprotection of minipig salivary glands by orciprenaline-carbachol

An ultrastructural and semiquantitative light microscopic study

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Summary

Parotid and submandibular glands of miniature pigs were exposed to 36 Gy X-irradiation given as 6×6 Gy in 3 weeks. Half of the animals received orciprenaline and carbachol before each dose. The effects were analysed 6 months later by light and electron microscopy. Ultrastructural examination showed less change in the pretreated glands. Semi-quantitative light microscopic data confirmed the significance of the differences. Acinar cells of both glands were significantly more numerous (P<0.01) and the cells were better preserved (P<0.01) in the pretreated group. The effect was more pronounced in the parotid gland, which appeared almost normal. Intercalated ducts of the parotid glands (P<0.01) and striated ducts of the submandibular glands (P<0.05) showed less change in pretreated animals. The findings confirm the radioprotective effect of pharmacologically induced degranulation of acinar cells. The biological effects of the radiation schedule (cumulative radiation effect value 18.76) as well as the dosage of orciprenaline and carbachol are within the normal range of medical treatment. Similar results may be expected from future studies in man.

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Authors and Affiliations

  1. Abteilung für Pathologie, Allgemeines Krankenhaus Altona, Paul-Ehrlich-Strasse 1, D-2000, Hamburg 50, Germany

    Suzanne Lotz & Jörg Caselitz

  2. Abteilung für Radiologie, Knappschaftskrankenhaus Bardenberg, Würselen, Germany

    Holger Tschakert

  3. Institut für Mathematik und Datenverarbeitung in der Medizin, Universität Hamburg, Hamburg, Germany

    Wolfgang Rehpenning

  4. Institut für Pathologie, Universität Hamburg, Hamburg, Germany

    Gerhard Seifert

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  1. Suzanne Lotz
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  2. Jörg Caselitz
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  3. Holger Tschakert
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  4. Wolfgang Rehpenning
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  5. Gerhard Seifert
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Supported by the Hamburger Stiftung zur Förderung der Krebsbekämpfung and by the Deutsche Forschungsgemeinschaft and by the “Otto Stiftung”

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Lotz, S., Caselitz, J., Tschakert, H. et al. Radioprotection of minipig salivary glands by orciprenaline-carbachol. Vichows Archiv A Pathol Anat 417, 119–128 (1990). https://doi.org/10.1007/BF02190529

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  • DOI: https://doi.org/10.1007/BF02190529

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