Photosynthesis Research

, Volume 37, Issue 2, pp 89–102 | Cite as

Can CO2 assimilation in maize leaves be predicted accurately from chlorophyll fluorescence analysis?

  • Gerald E. Edwards
  • Neil R. Baker
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Abstract

Analysis is made of the energetics of CO2 fixation, the photochemical quantum requirement per CO2 fixed, and sinks for utilising reductive power in the C4 plant maize. CO2 assimilation is the primary sink for energy derived from photochemistry, whereas photorespiration and nitrogen assimilation are relatively small sinks, particularly in developed leaves. Measurement of O2 exchange by mass spectrometry and CO2 exchange by infrared gas analysis under varying levels of CO2 indicate that there is a very close relationship between the true rate of O2 evolution from PS II and the net rate of CO2 fixation. Consideration is given to measurements of the quantum yields of PS II (φPS II) from fluorescence analysis and of CO2 assimilation (\(\phi _{CO_2 } \)) in maize over a wide range of conditions. The\({{\phi _{PSII} } \mathord{\left/ {\vphantom {{\phi _{PSII} } {\phi _{CO_2 } }}} \right. \kern-\nulldelimiterspace} {\phi _{CO_2 } }}\) ratio was found to remain reasonably constant (ca. 12) over a range of physiological conditions in developed leaves, with varying temperature, CO2 concentrations, light intensities (from 5% to 100% of full sunlight), and following photoinhibition under high light and low temperature. A simple model for predicting CO2 assimilation from fluorescence parameters is presented and evaluated. It is concluded that under a wide range of conditions fluorescence parameters can be used to predict accurately and rapidly CO2 assimilation rates in maize.

Key words

C4 photosynthesis chlorophyll fluorescence CO2 assimilation maize Photosystem II quantum yield 

Abbreviations

A

measured rate of CO2 assimilation

A* (=A+RL)

the rate of CO2 assimilation corrected for dark-type mitochondrial respiration

Fm

maximal yield of PS II chlorophylla fluorescence resulting from a saturating flash of white light under a given steady-state photosynthesis

Fs

variable yield of fluorescence under steady-state photosynthesis

IL

light absorbed by the leaf

IPS II

light absorbed by PS II

Jc

rate of whole chain electron transport

JO2

rate of O2 evolution from PS II

PPFD

photosynthetic photon flux density

RD

rate of mitochondrial respiration in the dark

RL

rate of respiration in the light (independent of photorespiration)

Rubisco

ribulose 1,5-bisphosphate carboxylase/oxygenase

vc

velocity of Rubisco carboxylase

vo

velocity of Rubisco oxygenase

vpr

velocity of CO2 release by photorespiration

\(\phi _{CO_2 } \)

apparent quantum yield of CO2 assimilation (A/absorbed PPFD)

\(\phi _{CO_2 ^ \cdot } \)

quantum yield of CO2 assimilation corrected for dark-type respiration [(A+RL)/absorbed PPFD]

\(\phi _{O_2 ^ \cdot } \)

quantum yield for photosynthetic O2 evolution

ΦPS II

quantum yield of PS II photochemistry (electron flux through PS II/rate of photon absorption by PS II antennae)

α

\({{\alpha - \phi _{PSII} } \mathord{\left/ {\vphantom {{\alpha - \phi _{PSII} } {\phi _{CO_2 ^ \cdot } }}} \right. \kern-\nulldelimiterspace} {\phi _{CO_2 ^ \cdot } }}\) ratio

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References

  1. Andrews JR, Bredenkamp GJ and Baker NR (1993) Evaluation of the role of state transitions in determining the efficiency of light utilization of CO2 assimilation in leaves. Photosynth Res (submitted)Google Scholar
  2. Badger MR (1985) Photosynthetic oxygen exchange. Ann Rev Plant Physiol 36: 27–53Google Scholar
  3. Badger MR and Canvin DT (1981) Oxygen uptake during photosynthesis in C3 and C4 plants. In: Akoyunoglou G (ed) Proc. 5th Intl Cong Photosynth, Vol IV, pp 151–161. Balaban Intl Sci Serv, PhiladelphiaGoogle Scholar
  4. Baker NR and Leech RM (1977) Development of Photosystem I and Photosystem II activities in leaves of light-grown maize (Zea mays). Plant Physiol 60: 640–644Google Scholar
  5. Baker NR and Ort DR (1993) Light and crop photosynthetic performance. In: Baker NR and Thomas H (eds) Crop Photosynthesis: Spatial and Temporal Determinants. Vol 12, Topics in Photosynthesis, pp 289–312, Elsevier, LondonGoogle Scholar
  6. Brooks A and Farquhar GD (1985) Effect of temperature on the CO2/O2 specificity of ribulose-1,5-bisphosphate carboxylase/oxygenase and the rate of respiration in the light. Planta 165: 397–406Google Scholar
  7. Byrd GT, Sage RF and Brown RH (1992) A comparison of dark respiration between C3 and C4 plants. Plant Physiol 100: 191–198Google Scholar
  8. Collatz GJ, Ribas-Carbo M and Berry JA (1992) Coupled photosynthesis-stomatal conductance model for leaves of C4 plants. Aust J Plant Physiol 19: 519–538Google Scholar
  9. Demmig B and Björkman O (1987) Comparison of the effect of excessive light on chlorophyll fluorescence (77K) and photon yield of O2 evolution in leaves of higher plants. Planta 171: 171–184Google Scholar
  10. De Veau EJ and Burris JE (1989) Photorespiratory rates in wheat and maize as determined by18O-labeling. Plant Physiol 90: 500–511Google Scholar
  11. Edwards GE and Walker DA (1983) C3, C4: Mechanisms, and Cellular and Environmental Regulation of Photosynthesis. Blackwell, Oxford, 542 ppGoogle Scholar
  12. Ehleringer J and Pearcy RW (1983) Variation in quantum yield for CO2 uptake among C3 and C4 plants. Plant Physiol 73: 555–559Google Scholar
  13. Furbank RT and Badger RT (1982) Photosynthetic oxygen exchange in attached leaves of C4 monocotyledons. Aust J Plant Physiol 9: 553–558Google Scholar
  14. Furbank RT and Badger RT (1983) Photorespiratory characteristics of isolated bundle sheath strands of C4 monocotyledons. Aust J Plant Physiol 10: 451–458Google Scholar
  15. Furbank RT, Badger MR and Osmond CB (1983) Photoreduction of oxygen in mesophyll chloroplasts of C4 plants. Plant Physiol 73: 1038–1041Google Scholar
  16. Furbank RT, Jenkins CLD and Hatch MD (1990) C4 photosynthesis: Quantum requirement, C4 acid overcycling and Q-cycle involvement. Aust J Plant Physiol 17: 1–7Google Scholar
  17. Genty B, Briantais J-M and Baker NR (1989) The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. Biochim Biophys Acta 990: 87–92Google Scholar
  18. Henderson SA, von Caemmerer S and Farquhar GD (1992) Short-term measurements of carbon isotope discrimination in several C4 species. Aust J Plant Physiol 19: 263–285Google Scholar
  19. Krall JP and Edwards GE (1992) Relationship between Photosystem II activity and CO2 fixation in leaves. Physiol Plant 86: 180–187Google Scholar
  20. Ku MSB and Edwards GE (1978) Oxygen inhibition of photosynthesis. III. Temperature dependence of quantum yield and its relation to O2/CO2 solubility ratio. Planta 140: 1–6Google Scholar
  21. Lawlor DW and Fock H (1978) Photosynthesis, respiration, and carbon assimilation in water-stress maize at two oxygen concentrations. J Exp Bot 29: 579–593Google Scholar
  22. Long SP, East TM and Baker NR (1983) Chilling damage to photosynthesis in youngZea mays. J Exp Bot 34: 177–188Google Scholar
  23. Miranda V, Baker NR and Long SP (1981) Limitations of photosynthesis in different regions of theZea mays leaf. New Phytol 89: 179–190Google Scholar
  24. Oberhuber W and Edwards GE (1993) Temperature dependence of the linkage of quantum yield of Photosystem II to CO2 fixation in C4 and C3 plants. Plant Physiol 101: 507–512PubMedGoogle Scholar
  25. Oberhuber W, Dai Z and Edwards GE (1993) Light dependence of quantum yields of Photosystem II and CO2 fixation in C3 and C4 plants. Photosynth Res 35: 265–274Google Scholar
  26. Öquist G and Chow WS (1992) On the relationship between the quantum yield of Photosystem II electron transport, as determined by chlorophyll fluorescence and the quantum yield of CO2-dependent O2 evolution. Photosynth Res 33: 51–62Google Scholar
  27. Ortiz-Lopez A, Nie GY, Ort DR and Baker NR (1990) The involvement of photoinhibition of Photosystem II and impaired membrane energization in the reduced quantum yield of carbon assimilation in chilled maize. Planta 181: 78–84Google Scholar
  28. Pearcy RW and Ehleringer J (1984) Comparative ecophysiology of C3 and C4 plants. Plant Cell Environ 7: 1–13Google Scholar
  29. Rees D and Horton P (1990) The mechanisms of changes in Photosystem II efficiency in spinach thylakoids. Biochem Biophys Acta 1016: 219–227Google Scholar
  30. Seaton GR and Walker DA (1990) Chlorophyll fluorescence as a measure of photosynthetic carbon assimilation. Philos Trans R Soc Lond B 242: 29–35Google Scholar
  31. Seaton GR and Walker DA (1992) Validating chlorophyll fluorescence measures of efficiency: Observations on fluorimetric estimation of photosynthetic rate. Proc R Soc Lond B 249: 41–47Google Scholar
  32. Sharp RE, Matthews MA and Boyer JS (1984) Kok effect and the quantum yield of photosynthesis. Light partially inhibits dark respiration. Plant Physiol 75: 95–101Google Scholar
  33. Tobin AK (1992) Carbon and nitrogen metabolism: interactions during leaf development. In: Baker NR and Thomas H (eds) Crop Photosynthesis: Spatial and Temporal Determinants. Vol 12, Topics in Photosynthesis, pp 381–412. Elsevier, LondonGoogle Scholar
  34. Walker DA (1993) Polarographic measurement of oxygen. In: Hall DO, Scurlock JMO, Bolhar-Nordenkampf HR, Leegood RC and Long SP (eds) Photosynthesis and Production in a C-hanging Environment, pp 168–180. Chapman and Hall, LondonGoogle Scholar

Copyright information

© Kluwer Academic Publishers 1993

Authors and Affiliations

  • Gerald E. Edwards
    • 1
  • Neil R. Baker
    • 2
  1. 1.Botany DepartmentWashington State UniversityPullmanUSA
  2. 2.Department of BiologyUniversity of EssexColchesterUK

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