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Differences between strains of rhizobium in sensitivity to canavanine

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Summary

Four strains of rhizobia that nodulate canavanine-synthesizing legumes and four strains that nodulate noncanavanine-synthesizing legumes were tested for sensitivity to L-canavanine. The effect of canavanine on growth depends upon the strain of Rhizobium tested rather than the canavanine synthesizing capability of the host legume. In both groups of rhizobia, some strains were inhibited in growth by canavanine. Canavanine enhancement of growth was observed in rhizobia that nodulate noncanavanine-synthesizing legumes.

Canavanine was found to enhance incorporation of uridine-H3 and L-leucine-H3 into trichloroacetic acid insoluble fractions of starved cells of two strains of rhizobia tested. This demonstrated that under certain conditions some rhizobia can detoxify canavanine and utilize it in synthetic processes. re]19760729

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References

  1. Birdsong, B. A., Alston, R. and Turner, B. L., Distribution of canavanine in the family Leguminosae as related to phyletic groupings. Can. J. Bot. 38, 499–505 (1960).

    Google Scholar 

  2. Fowden, L., Lewis, D. and Tristram, H., Toxic amino acids: Their action as antimetabolites. Adv. Enzymol. Relat. Areas Mol. Biol. 29, 89–163 (1967).

    PubMed  Google Scholar 

  3. Grenson, M. and Hennaut, C., Mutations affecting activity of several distinct amino acid transport systems inSaccharomyces cerevisiae. J. Bacteriol. 105, 477–482 (1970).

    Google Scholar 

  4. Hare, J. D., Reversible inhibition of DNA synthesis by the arginine analogue canavanine in hamster and mouse cellsin vitro. Exp. Cell Res. 58, 170–174 (1969).

    PubMed  Google Scholar 

  5. Kalyankar, G. D., Ikawa, M., and Snell, E. E., The enzymatic cleavage of canavanine to homoserine and hydroxyguanidine. J. Biol. Chem. 233, 1175–1178 (1958).

    PubMed  Google Scholar 

  6. Konobu, K., Antagonism between arginine and canavanine on adaptive formation of formic hydrogenlyase inEscherichia coli No. 1. Yakuguka Zasshi 83, 33–36 (1963).

    Google Scholar 

  7. Kruse, P. F., White, P. B., Carter, H. A. and McCoy, T. A., Incorporation of canavanine into protein of Walker carcinosarcoma 256 cells culturesin vitro. Cancer Res. 19, 122–125 (1959).

    PubMed  Google Scholar 

  8. Lockhart, W. R. and Garner, H. R., Genetic mechanisms governing the effect of canavanine onNeurospora crassa. Genetics 40, 721–725 (1955).

    Google Scholar 

  9. Phillips, D. A. and Torrey, J. G., Cytokinin production byRhizobium japonicum. Physiol. Plant. 23, 1057–1063 (1970).

    Google Scholar 

  10. Rosenthal, G. A., Investigations of canavanine biochemistry in the jack bean plant,Canavalia ensiformis (L.) DC. I. Canavanine utilization in the developing plant. Plant Physiol. 46, 273–276 (1970).

    Google Scholar 

  11. Rosenthal, G. A., Investigations of canavanine biochemistry in the jack bean plant,Canavalia ensiformis (L.) DC. II. Canavanine biosynthesis in the developing plant. Plant Physiol. 50, 328–331 (1972).

    Google Scholar 

  12. Rosenthal, G. A., Gulati, D. K. and Sabharwal, P. S., Studies on the growth effects of the canaline-urea cycle amino acids withLemna minor L. Plant Physiol. 56, 420–424 (1975).

    Google Scholar 

  13. Simonnet, G. M. and Chapeville, F., Action de la canavanine sur la synthese du RNA chezE. coli. Eur. J. Biochem. 9, 199–206 (1969).

    PubMed  Google Scholar 

  14. Sköld, O. and Zetterberg, A., Studies on the amino acid regulation of RNA synthesis in mammalian cells in tissue culture. Exp. Cell Res. 55, 289–294 (1969).

    PubMed  Google Scholar 

  15. Stent, G. and Brenner, S., A genetic locus for the regulation of RNA synthesis. Proc. Nat. Acad. Sci. U.S.A. 47, 2005–2014 (1961).

    Google Scholar 

  16. Teas, H. H., Effect of canavanine on mutants ofNeurospora andBacillus subtilis. J. Biol. Chem. 190, 368–375 (1951).

    Google Scholar 

  17. Turner, B. L. and Harborne, J. B., Distribution of canavanine in the plant kingdom. Phytochem. 6, 863–866 (1967).

    Google Scholar 

  18. Valera, C. L. and Alexander, M., Nodulation factor forRhizobium-legume symbiosis. J. Bacteriol. 89, 1134–1139 (1965).

    PubMed  Google Scholar 

  19. Weaks, T. E., Effects of canavanine on arginine utilization in plants with special reference to mitotic activity. Physiol. Plant. 31, 144–148 (1974).

    Google Scholar 

  20. Weaks, T. E. and Hunt, G. E., The effects of canavanine on protein and nucleic acid synthesis in canavanine resistant and sensitive species of higher plants. Physiol. Plant. 29, 421–424 (1973).

    Google Scholar 

  21. Weaks, T. E. and Hunt, G. E., The effects of canavanine on the growth of isolated roots of four plant species. Bot. Gaz. 135, 18–21 (1974).

    Google Scholar 

  22. Williams, S. E. and Hunt, G. E., Canavanine distribution in jackbean fruit during fruit growth. Planta 77, 192–202 (1967).

    Google Scholar 

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  1. Department of Biological Sciences, Marshall University, 25701, Huntington, West Virginia, USA

    Thomas E. Weaks

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  1. Thomas E. Weaks
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Weaks, T.E. Differences between strains of rhizobium in sensitivity to canavanine. Plant Soil 48, 387–395 (1977). https://doi.org/10.1007/BF02187248

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