Isolation and laboratory culture ofMicrocystis aeruginosa Kütz. using a growth medium (MLA medium) suitable for both non-axenic and axenic cultures is described. Seventeen established strains ofM. aeruginosa were subjected to one or more of three purification methods: centrifugation cleaning, sulphide gradient selection, and antibiotic treatment (Imipenem®). While each method purified only about half of the strains attempted, the selective application of each method, based on the morphological characteristics of the strains, succeeded in purifying 12 of the 17 strains. Three of the 5 strains not purified were contaminated with a sulphide-tolerant, Imipenem-resistant spirochaete,Spirochaeta cf.aurantia, which could not be detected on normal, broad spectrum bacterial test media. The presence of this bacterial species was detected only by phase contrast and DAPI (4,6-diamidino-2-phenylindole) stained fluorescence microscopy.
This is a preview of subscription content, access via your institution.
Buy single article
Instant access to the full article PDF.
Price excludes VAT (USA)
Tax calculation will be finalised during checkout.
Allen MM (1968) Simple conditions for growth of unicellular bluegreen algae on plates. J. Phycol. 4: 1–4.
Baker PD (1992) Identification of common noxious Cyanobacteria. Part II — Chroococcales and Oscillatoriales. Urban Water Research Association of Australia Research Report 46.
Boon PI (1992) Antibiotic resistance of aquatic bacteria and its implications for limnological research. Aust. J. mar. freshwater Res. 43: 847–859.
Canale-Parola (1981) Free-living anaerobic and facultatively anaerobic Spirochaetes: The genusSpirochaeta. In Starr MP, Stolp H, Trüper HG, Balows A, Schlegel HG (eds), The Prokaryotes, Vol. 1. A Handbook on Habits, Isolation and Identification of Bacteria. Springer-Verlag, Berlin, Germany: 538–547.
Carmichael WW (1992) Cyanobacteria secondary metabolites — the cyanotoxins. J. appl. Bact. 72: 445–459.
Castenholz RW (1988) Culturing methods for cyanobacteria. Meth. Enzymol. 167: 68–92.
Ferris MJ, Hirsch CF (1991) Method for isolation and purification of cyanobacteria. Appl. environ. Microbiol. 57: 1448–1452.
Fogg GE (1942) Studies on nitrogen fixation by blue-green algae. 1. Nitrogen fixation byAnabaena cylindrica Lemm. J. exp. Biol. 19: 78–87.
Gorham PR, McLachlan J, Hammer UT, Kim WK (1964) Isolation and culture of toxic strains ofAnabaena flos-aquae (Lyngb.) de Bréb. Verb. int. Ver. Limnol. 15: 796–804.
Guillard RRL (1973) Methods for microflagellates and nanoplankton. In Stein JR (ed) Handbook of Phycological Methods: Culture methods and growth measurements. Cambridge University Press, London, UK: 69–86.
Hoshaw RW Rosowski JR (1973) Methods for microscopic algae. In Stein JR (ed.) Handbook of Phycological Methods: Culture methods and growth measurements. Cambridge University Press, London, UK: 53–68.
Mudaliar U, Furyk N, Wilson V, Infanti J, Mayall B (1994) Comparative in-vitro antibacterial activity of Meropenem. In Abstracts of Australian Society for Microbiology Annual Scientific Meeting, Melbourne, 1994, Abstract P02.32, Aust. Microbiol. 15(4): 132.
Parker DL (1982) Improved procedures for the cloning and purification ofMicrocystis cultures (Cyanophyta). J. Phycol. 18: 471–477.
Porter KG, Feig YS (1980) The use of DAPI for identifying and counting aquatic microflora. Limnol. Oceanogr. 25: 943–948.
Rippka R (1988) Isolation and purification of cyanobacteria. Meth. Enzymol. 167: 3–27.
Rippka R, Waterbury JB, Stanier RY (1981) Isolation and purification of cyanobacteria: Some general principles. In Starr MP, Stolp H, Trüper HG, Balows A, Schlegel HG (eds), The Prokaryotes, Vol. 1. A Handbook on Habits, Isolation and Identification of Bacteria. Springer-Verlag, Berlin, Germany: 212–220.
Simon RD (1987) Inclusion bodies in the cyanobacteria: cyanophycin, polyphosphate, polyhedral bodies. In Fay P, Van Baalen C (eds), The Cyanobacteria. Elsevier Science Publishers B.V, Amsterdam, Netherlands: 199–226.
Shirai M, Matumaru K, Ohotake A, Takamura Y, Aida T, Nakano M (1989) Development of a solid medium for growth and isolation of axenicMicrocystis strains (Cyanobacteria). Appl. environ. Microbiol. 55: 2569–2571.
Shirai M, Ohtake A, Takaai S, Matsumoto S, Sakamoto T, Sato A, Aida T, Harada K, Shimada T, Suzuki M, Nakano M (1990) Toxicity and toxins of natural blooms and isolated strains ofMicrocystis spp. (Cyanobacteria) and improved procedure for purification of cultures. Appl. environ. Microbiol. 57: 1241–1245.
Vaara T, Vaara M, Niemela S (1979). Two improved methods for obtaining axenic cultures of cyanobacteria. Appl. environ. Microbiol. 38: 1011–1014.
Author for correspondence
About this article
Cite this article
Bolch, C.J.S., Blackburn, S.I. Isolation and purification of Australian isolates of the toxic cyanobacteriumMicrocystis aeruginosa Kütz. J Appl Phycol 8, 5–13 (1996). https://doi.org/10.1007/BF02186215