Journal of Applied Phycology

, Volume 6, Issue 1, pp 35–39 | Cite as

Protoplast production fromPorphyra linearis using a simplified agarase procedure capable of commercial application

  • L. C.-M Chen
  • J. S. Craigie
  • Z. K. Xie


Abalone enzymes, Cellulase R-10, Macerozyme and agarase fromPseudomonas atlantica were tested for activity on agarose, cellulose, xylan, the cell wall matrix and porphyran isolated fromPorphyra linearis. Agarase, and to a lesser extent Macerozyme, digested both agarose and porphyran. Abalone enzymes and Cellulase R-10 reacted only weakly with porphyran. A simple standardized protocol for making protoplasts fromPorphyra linearis was developed using 0.025% agarase in seawater without added organic osmoticants. Protoplasts prepared with agarase remained viable for at least 24 h in the digestion medium. Regeneration of the protoplasts followed the normal pattern for this species. Agarase can be used to obtain large number of protoplasts which could substitute for conchospores in seeding nets for the aquaculture ofP. linearis

Key words

protoplast agarase enzyme aquaculture Porphyra Pseudomonas 


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Copyright information

© Kluwer Academic Publishers 1994

Authors and Affiliations

  • L. C.-M Chen
    • 1
  • J. S. Craigie
    • 1
  • Z. K. Xie
    • 2
  1. 1.Institute for Marine Biosciences, National Research Council of CanadaHalifaxCanada
  2. 2.Department of BiologyNanjing UniversityNanjingPeople's Republic of China

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