Journal of Applied Phycology

, Volume 4, Issue 4, pp 385–389 | Cite as

High performance liquid chromatography analysis of diarrhetic toxins inDinophysis spp. from the French coast

  • Pierre Masselin
  • Patrick Lassus
  • Michèle Bardouil
Article

Abstract

Diarrhetic shellfish poisoning has been a recurrent problem along the Brittany coast (France) since 1983. Okadaic acid (O.A.), the main toxin detected in mussels, is generally associated with the presence ofDinophysis cells in sea water. We report here the results of okadaic acid analyses by high performance liquid chromatography, on planktonic samples collected during the summer of 1991.D. sacculus, the majorDinophysis species, throughout this period, showed low okadaic acid content in raw extract, whereas toxic levels were 3- to 12-fold higher in sorted samples than in raw ones. A maximum O.A. concentration of 29.6 pg cell-1 was found inD. sacculus/D. cf. acuminata extract. Similarly, higher O.A. levels were noted in raw samples when these two species were associated. Generally speaking, variations in raw samples were similar to those in sorted samples. Nevertheless, whenD. rotundata reached a concentration equal to that ofD. sacculus in sorted samples, the O.A. level was lower.

Key words

dinoflagellate diarrhetic shellfish poisoning okadaic acid Dinophysis 

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Copyright information

© Kluwer Academic Publishers 1992

Authors and Affiliations

  • Pierre Masselin
    • 1
  • Patrick Lassus
    • 1
  • Michèle Bardouil
    • 1
  1. 1.Centre de NantesIFREMERNantes Cedex 01France

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