Effects of soil acidity on nodulation ofAlnus glutinosa and viability ofFrankia
Black alder seedlings were grown from seed for 7 weeks in six soils limed to various pH levels and inoculated withFrankia in two inoculation-seeding time combinations (inoculated and seeded concurrently; inoculated then seeded 5 weeks after inoculation). Three mine soils and three non-mine soils were used. Soil pHs in the study ranged from 3.6 to 7.6. In the second inoculation-seeding time combination, a series of soil samples at each of the pH levels below 7.0 were relimed to pH 7.0 immediately prior to seeding. The purpose of the study was to examine the effects of soil acidity on the nodulation of black alder byFrankia and the viability ofFrankia in acid soils. Based on the average number of nodules established per seedling, soil pH was determined to be a significant factor affecting nodulation in the mine soils. The highest levels of nodulation occurred between soil pH 5.5 and 7.2. Below pH 5.5, nodulation was reduced. There was also evidence of decreased viability of the endophyte below pH 4.5.
Key wordsAlnus glutinosa Frankia Nodulation Soil acidity
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- 1.Bond G 1951 The fixation of nitrogen associated with the root nodules ofmyrica gale L., with special reference to its pH relation and ecological significance. Ann. Bot. 15, 447–459.Google Scholar
- 3.Burggraaf A J P and Shipton W A 1982 Estimates ofFrankia growth under various pH and temperature regimes. Plant and Soil 69, 135–147.Google Scholar
- 5.Ferguson T P and Bond G 1953 Observations on the formation and function of the root nodules ofAlnus glutinosa (L.) Gaertn. Ann. Bot. 17, 175–187.Google Scholar
- 6.Lalonde M 1979 Techniques and observations of the nitrogen-fixing Alnus root nodule symbiosis.In Recent Advances in Biological Nitrogen Fixation. Ed. N S Subba Rao. Oxford and IBH Publ. Co., New Delhi, pp 421–434.Google Scholar
- 7.Quispel A 1954 Symbiotic nitrogen fixation in non-leguminous plants; II. The influence of some environmental conditions on different phases of the nodulation process inAlnus glutinosa. Acta Bot. Neerl. 7, 191–204.Google Scholar
- 8.Rodriquez-Barrueco C 1968 The occurrence of the root-nodule endophytes ofAlnus glutinosa andMyrica gale in soils. J. Gen. Microbiol. 52, 189–194.Google Scholar