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Structures of chlorosomes and aggregated BChlc inChlorobium tepidum from solid state high resolution CP/MAS13C NMR

Photosynthesis Research volume 41, pages 211–223 (1994)Cite this article

Abstract

Cross polarization/magic angle spinning (CP/MAS)13C (solid state high resolution) NMR spectra were observed for chlorosomes and BChlc aggregates. Similarity of both kinds of spectra (except for some signals assignable to proteins and lipids in chlorosomes) indicates that BChlc's in chlorosomes are present just as in synthetic BChlc aggregates. Chemical shifts for C131 carbonyl and C31 hydroxylethyl carbons indicate hydrogen bonding between them. Comparison of solution and solid state13C NMR chemical shifts shows the five coordinated nature of BChlc aggregates. Some chemical shift differences were attributable to ring currents shifts. Their comparisons with calculated ring current shift values predicted structures for the aggregates. Cross polarization dynamics of the CP/MAS13C NMR signals explored dynamic and structural nature of the BChlc aggregates.

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Abbreviations

BChl:

bacteriochlorophyll

CP:

cross polarization

MAS:

magic angle spinning

TMS:

tetramethylsilane

TOSS:

total elimination of spinning side bands

TCH :

cross polarization time constant between C and H

T1ϱH:

spin lattice relaxation time in the rotating frame

τ:

contact time

τm :

mixing time

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Affiliations

  1. Department of Biochemistry and Engineering, Faculty of Engineering, Tohoku University, 980, Sendai, Japan

    Tsunenori Nozawa, Katsunori Ohtomo, Manabu Suzuki, Hiroshi Nakagawa, Yuko Shikama & Zheng-Yu Wang

  2. Institute for Chemical Reaction Science, Tohoku University, 980, Sendai, Japan

    Hideo Konami

Authors
  1. Tsunenori Nozawa
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  2. Katsunori Ohtomo
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  3. Manabu Suzuki
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  4. Hiroshi Nakagawa
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  5. Yuko Shikama
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  6. Hideo Konami
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  7. Zheng-Yu Wang
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Nozawa, T., Ohtomo, K., Suzuki, M. et al. Structures of chlorosomes and aggregated BChlc inChlorobium tepidum from solid state high resolution CP/MAS13C NMR. Photosynth Res 41, 211–223 (1994). https://doi.org/10.1007/BF02184162

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  • DOI: https://doi.org/10.1007/BF02184162

Key words

  • antenna structure
  • green photosynthetic bacteria
  • cross polarization
  • magic angle spinning
  • ring current shift