Skip to main content

Pigment interactions in chlorosomes of various green bacteria

Photosynthesis Research volume 41, pages 175–180 (1994)Cite this article

Abstract

Resonance Raman experiments were performed on different green bacteria. With blue excitation, i.e. under Soret resonance or preresonance conditions, resonance Raman contributions were essentially arising from the chlorosome pigments. By comparing these spectra and those of isolated chlorosomes, it is possible to evaluate how the latter retain their native structure during the isolation procedures. The structure of bacteriochlorophyll oligomers in chlorosomes was interspecifically compared, in bacteriochlorophyllc- and bacteriochlorophylle- synthesising bacteria. It appears that interactions assumed by the 9-keto carbonyl group are identical inChlorobium limicola, Chlorobium tepidum, andChlorobium phaeobacteroides. In the latter strain, the 3-formyl carbonyl group of bacteriochlorophylle is kept free from intermolecular interactions. By contrast, resonance Raman spectra unambiguously indicate that the structure of bacteriochlorophyll oligomers is slightly different in chlorosomes fromChloroflexus auranticus, either isolated or in the whole bacteria.

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

43,34 €

Price includes VAT (Finland)
Tax calculation will be finalised during checkout.

Abbreviations

BChl:

bacteriochlorophyll

Chl:

chlorophyll

Cb. :

chlorobium

Cf. :

Chloroflexus

fwhm:

full width half maximum

RR:

resonance Raman

References

  • Biebl H and Pfennig N (1978) Growth yields of green sulfur bacteria in mixed cultures with sulfur and sulfate reducing bacteria. Arch Microbiol 117: 9–16

    Google Scholar 

  • Bystrova MI, Malghosheva IN and Krasnovskii AA (1979) Study of molecular mechanisms of self assembly of aggregated forms of bacteriochlorophyllc. Mol Biol USSR 13: 582–594

    Google Scholar 

  • Cohen-Bazire G, Pfennig N and Kunizawa R (1964) The fine structure of green bacteria. J Cell Biol 22: 207–221

    Google Scholar 

  • Cotton TM and vanDuyne RP (1981) Characterization of bacteriochlorophyll interactions in vitro by resonance Raman spectroscopy. J Am Chem Soc 103: 6020–6026

    Google Scholar 

  • Feiler U, Pourcet C, Mattioli TA, Lutz M and Robert B (1994) Structure and binding site of the primary acceptor in the reaction center ofChlorobium. Biochemistry (in press)

  • Gerola P and Olson JM (1986) A new bacteriochlorophylla-protein complex associated with chlorosomes of green sulfur bacteria. Biochim Biophys Acta 848: 69–76

    Google Scholar 

  • Griebenow K and Holzwarth AR (1989) Pigment organization and energy transfer in green bacteria 1. Isolation of native chlorosomes free of bound bacteriochlorophylla fromChloroflexus aurantiacus by gel electrophoretic filtration. Biochim Biophys Acta 973: 235–240

    Google Scholar 

  • Griebenow K and Holzwarth AR (1990) The 5.6 kilodalton protein in isolated chlorosomes ofChloroflexus aurantiacus strain OK-70-FL is a degradation product. Z Naturforsch 45: 823–828

    Google Scholar 

  • Hildebrandt P, Griebenow K, Holzwarth AR and Schaffner K (1991) Resonance Raman spectroscopic evidences for the identity of the bacteriochlorophyllc organization in protein free containing chlorosomes fromChloroflexus aurantiacus. Z Naturforsch 46: 228–232

    Google Scholar 

  • Hojrup P, Gerola P, Hansen HF, Mikkelsen JM, Shahed AE, Knudsen J, Roepstorff P and Olson JM (1991) The aminoacid sequence of a major protein component in the light harvesting complex of the green photosynthetic bacteriumChlorobium limicola f. thiosulfatophila. Biochim Biophys Acta 1077: 220–224

    Google Scholar 

  • Holzwarth AR, Griebenow K and Schaffner K (1990) A photosynthetic antenna system which contains a protein-free chromophore aggregate. Z Naturforsch 45: 203–206

    Google Scholar 

  • Lutz M (1979) Diffusion Raman de résonance des chlorophylles: applications à l'étude de l'organisation de la membrane photosynthétique. Thèse de doctorat d'état, Université Pierre et Marie Curie Paris VI

  • Lutz M (1984) Resonance Raman studies in photosynthesis. In: Clark RJH and Hester RE (eds) Advances in Infrared and Raman Spectroscopy, Vol 11, Ch 5, pp 211–300 John Wiley & Sons, Chichester

    Google Scholar 

  • Lutz M and Robert B (1988) Chlorophylls and the photosynthetic membrane. In: Spiro TG (ed) Biological Applications of Raman Spectroscopy, Vol III, Ch 8, pp 347–411. John Wiley & Sons, New York

    Google Scholar 

  • Lutz M and vanBrakel G (1988) Ground-state molecular interactions of bacteriochlorophyllc in chlorosomes of green bacteria and in model system: a resonance Raman study. In: Olson JM, Ormerod JG, Amesz J, Stackebrandt E and Trüper HG (eds) Green Photosynthetic Bacteria, pp 23–34. Plenum Press, New York and London

    Google Scholar 

  • Mattioli TA, Hoffmann A, Sockalingum DG, Schrader B, Robert B and Lutz M (1993) Application of near-IR Fourier transform resonance Raman spectroscopy to the study of photosynthetic proteins. Spectrochimica Acta 49A: 785–799

    Google Scholar 

  • Miller M, Simpson D and Redlinger TE (1993) The effect of detergent on the structure and composition of chlorosomes isolated fromChloroflexus aurantiacus. Photosynth Res 35: 275–283

    Google Scholar 

  • Niedermeier G, Scheer H and Feick RG (1992) The functional role of protein in the organization of bacteriochlorophyllc in chlorosomes ofChloroflexus aurantiacus. Eur J Biochem 204: 685–692

    Google Scholar 

  • Nozawa T, Noguchi T and Tasumi M (1990) Resonance Raman studies of bacteriochlorophyllc in chlorosomes fromChloroflexus aurantiacus. J Biochem (Tokyo) 108: 737–740

    Google Scholar 

  • Olson JM, Gerola PD, VanBrakel GH, Meiburg RF and Vasmel H (1985) Bacteriochlorophylla- andc- protein complexes from chlorosomes of green sulphur bacteria compared with bacteriochlorophyllc aggregates in dichloromethane-hexane. Springer Ser Chem Phys 42: 67–73

    Google Scholar 

  • Robert B and Lutz M (1986) Structure of the primary donor ofRhodopseudomonas sphaeroides: Difference resonance Raman spectroscopy of reaction centers. Biochemistry 25: 2303–2309

    Google Scholar 

  • Schmidt K (1981) A comparative study on the composition of chlorosomes and cytoplasmic membranes fromChloroflexus aurantiacus strain Ok-70-fl andChlorobium limicola f. thiosulfatophilum strain 6230

  • Smith KM, Kehres LA and Fajer J (1983) Aggregation of bacteriochlorophyllsc, d ande. Models for antenna chlorophylls of green and brown photosynthetic bacteria. J Am Chem Soc 105: 1387–1390

    Google Scholar 

  • Wahlund TM, Woese CR, Castenholz RW and Madigan MT (1991) A thermophilic green sulfur bacterium from New Zealand hot springs,Chlorobium tepidum sp. nov. Arch Microbiol 156: 81–90

    Google Scholar 

  • Wechsler T, Suter F, Fuller RC and Zuber H (1985) The complete aminoacid sequence of the bacteriochlorophyllc binding polypeptide from chlorosomes of the green photosynthetic bacteriumChloroflexus aurantiacus. FEBS Lett 181: 173–178

    Google Scholar 

  • Weiss CJr (1972) The Pi electron structure and absorption spectra of chlorophylls in solution. Journal of molecular spectroscopy 44: 37–80

    Google Scholar 

  • Widdel F, Kohring GW and Mayer F (1983) Studies on dissimilatory sulfate-reducing bacteria that decompose fatty acids. Arch Microbiol 134: 286–294

    Google Scholar 

  • Worcester DL, Michalski TJ and Katz JJ (1986) Small-angle neutron scattering studies of chlorophyll micelles models for bacterial antenna chlorophyll. Proc Natl Acad Sci USA 83: 3791–3795

    Google Scholar 

  • Wullink W, Knudsen J, Olson JM, Redlinger TE and VanBruggen EFJ (1991) Localisation of polypeptides in isolated chlorosomes from green phototrophic bacteria by immunogold labeling electron microscopy. Biochim Biophys Acta 1060: 97–105

    Google Scholar 

Download references

Author information

Affiliations

  1. Section de Biophysique des Protéines et des Membranes, DBCM/CEA et URA 1290 CNRS, C.E. Saclay, 91191, Gif/Yvette Cedex, France

    Ute Feiler, Delphine Albouy, Marc Lutz & Bruno Robert

Authors
  1. Ute Feiler
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Delphine Albouy
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Marc Lutz
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Bruno Robert
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Feiler, U., Albouy, D., Lutz, M. et al. Pigment interactions in chlorosomes of various green bacteria. Photosynth Res 41, 175–180 (1994). https://doi.org/10.1007/BF02184158

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02184158

Key words

  • Chlorobium
  • Chloroflexus
  • chlorophyll
  • light-harvesting
  • photosynthesis
  • resonance Raman