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Ammonium sensing in nitrogen fixing bacteria: Functions of theglnB andglnD gene products

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Abstract

A plentiful supply of fixed nitrogen as ammonium (or other compounds such as nitrate or amino acids) inhibits nitrogen fixation in free-living bacteria by preventing nitrogenase synthesis and/or activity. Ammonium and nitrate have variable effects on the ability ofRhizobiaceae (Rhizobium, Bradyrhizobium andAzorhizobium) species to nodulate legume hosts and on nitrogen fixation capacity in bacteroid cells contained in nodules or in plant-free bacterial cultures. In addition to effects on nitrogen fixation, excess ammonium can inhibit activity or expression of other pathways for utilization of nitrogenous compounds such as nitrate (through nitrate and nitrite reductase), or glutamine synthetase (GS) for assimilation of ammonium. This paper describes the roles of two key genesglnB andglnD, whose gene products sense levels of fixed nitrogen and initiate a cascade of reactions in response to nitrogen status. While work onEscherichia coli and other enteric bacteria provides the model system,glnB and, to a lesser extent,glnD have been studied in several nitrogen fixing bacteria. Such reports will be reviewed here. Recent results on the identity and function of theglnB andglnD gene products inAzotobacter vinelandii (a free-living soil diazotroph) and inRhizobium leguminosarum biovarviciae, hereinafter designatedR.l. viciae will be presented. New data suggests thatAzotobacter vinelandii probably contains aglnB-like gene and this organism may have twoglnD-like genes (one of which was recently identified and namednfrX). In addition, evidence for uridylylation of theglnB gene product (the PII protein) ofR. l. viciae in response to fixed nitrogen deficiency is presented. Also, aglnB mutant ofR. l. viciae has been isolated; its characteristics with respect to expression of nitrogen regulated genes is described.

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Abbreviations

KD:

kiloDaltons

kb:

kilobase

UMP:

uridine monophosphate

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Authors and Affiliations

  1. Department of Plant Pathology, University of Arizona, AZ85721, Tucson, USA

    Christina Kennedy, Natalie Doetsch & Dietmar Meletzus

  2. International Institute of Genetics and Biophysics, Via Marconi 10, 80125, Naples, Italy

    Eduardo Patriarca, Mohamad Amar & Maurizio Iaccarino

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  1. Christina Kennedy
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  2. Natalie Doetsch
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  3. Dietmar Meletzus
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  4. Eduardo Patriarca
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  5. Mohamad Amar
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  6. Maurizio Iaccarino
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Kennedy, C., Doetsch, N., Meletzus, D. et al. Ammonium sensing in nitrogen fixing bacteria: Functions of theglnB andglnD gene products. Plant Soil 161, 43–57 (1994). https://doi.org/10.1007/BF02183084

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