Advertisement

Plant and Soil

, Volume 55, Issue 3, pp 353–361 | Cite as

Evaluation of extractants for soil humic substances

I. Isotachophoretic studies
  • Gustavo A. Orioli
  • Néstor R. Curvetto
Article

Summary

An evaluation of alkaline extractants was done by isotachophoresis and compared with infrared, ultraviolet and visible spectroscopic analysis. The electrophoretograms show that the humic acid extracted. This result is partially corroborated by the E4/E6 ratio. Differences in the carboxyl content and TRIS. In the pyrophosphate humic acid three fractions of high molecular weight were not extracted. This resultis partially corroborated by the E4/E6 ratio. Differences in the carboxyl content found by ir spectra of the TRIS humic acid and the NaOH humic acid were not shown in the isotachophoretic separation.

Key Words

Electrophoresis Extractants Isotachophoresis Humic acid 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Curvetto, N., Balmaceda, N. A. and Orioli, G. A. 1975 Electrophoretic methods for fractionation of humic and fulvic acids. Turrialba25, 265–270.Google Scholar
  2. 2.
    Evereaerts, F. M. and Routs, R. J. 1971 Calculation and measurement of concentrations in isotachophoresis. J. Chromatog.58, 181–194.Google Scholar
  3. 3.
    Felbeck, G. T. Jr. 1965 Structural chemistry of soil humic substances. Adv. Agron.17, 327–368.Google Scholar
  4. 4.
    Griffith, A., Catsimpolas, N. and Kerney, J. 1973 Analytical gel isotachophoresis of proteins with carrier anpholites spacers. Ann. N. Y. Acad. Sci.209, 457–469.Google Scholar
  5. 5.
    Haworth, R. D. 1971 The chemical nature of humic acid. Soil Sci.111, 71–79.Google Scholar
  6. 6.
    Hjamarsson, S. 1975 Preparative isotachophoresis—The effect of using ampholine of different pH ranges as spacer ions in the fractionation of serum proteins. Sci. Tools22, 35–38.Google Scholar
  7. 7.
    Kononova, M. M. 1966 Soil organic matter. Oxford, Pergamon Press.Google Scholar
  8. 8.
    Kononova, M. M. 1967 Methods of determining humus composition and their rationalization. Sov. Soil Sci. 7, 894–903.Google Scholar
  9. 9.
    Levesque, M. 1966 Effects of NaOH concentration on the extraction of organic matter and of major inorganic constituents from a soil. Can. J. Soil Sci.45, 7–12.Google Scholar
  10. 10.
    Ortiz de Serra, M. I. and Schnitzer, M. 1972 Extraction of humic acid by alkali and chelating resin. Can J. Soil Sci.52, 365–374.Google Scholar
  11. 11.
    Ortiz de Serra, M. I. and Schnitzer, M. 1973 The chemistry of humic and fulvic acids extracted from Argentine soils—I. Analytical characteristics. Soil Biol. Biochem.5, 281–286.Google Scholar
  12. 12.
    Ponomareva, V. V. and Plotnikova, T. A. 1968 Methods and some results of the fractionation of Chernozem humus. Sov. Soil Sci.11, 1562–1572.Google Scholar
  13. 13.
    Posner, A. M. 1966 The humic acids extracted by various reagents from a soil. Part I. Yield, inorganic components, and titration curves. J. Soil Sci.17, 65–78.Google Scholar
  14. 14.
    Schnitzer, M. 1965 The application of infrared spectroscopy to investigations on soil humic compounds. Cand. Spectrosc.10, 121–127.Google Scholar
  15. 15.
    Schnitzer, M. and Khan, S. U. 1972 Humic substances in the environment. Marcel dekker, Inc., New York.Google Scholar
  16. 16.
    Schnitzer, M. and Skinner, S. I. M. 1968 Alkaliversus acid extraction of soil organic matter. Soil Sci.105, 392–396.Google Scholar
  17. 17.
    Swift, R. S. and Posner, A. M. 1972 Autoxidation of humic acid under alkaline conditions. J. Soil. Sci.23, 381–303.Google Scholar
  18. 18.
    Theng, B. K. G., Wake, J. R. H. and Posner, A. M. 1967 The humic acids extracted by various reagents from a soil. Part II. Infrared, visible, and ultraviolet absorption spectra. J. Soil Sci.18, 349–363.Google Scholar

Copyright information

© Martinus Nijhoff Publishers 1980

Authors and Affiliations

  • Gustavo A. Orioli
    • 1
  • Néstor R. Curvetto
    • 1
  1. 1.Departmento de Ciencias AgrariasUniversidad Nacional del SurBahía BlancaArgentina

Personalised recommendations