Résumé
En vue d'une étude, au laboratoire, de la nodulation et de la symbiose plante-bactérie des préparations de protoplastes de mésophylle et de nodule racinaire ont été effectuées chez le haricot (Phaseolus vulgaris variété Constant). Pour chaque préparation, les conditions opératoires et les concentrations optimales de cellulase et de pectinase en solution, dans du mannitol 0,6M ont été définies.
Áprès 15 jours, 10 à 15% des protoplastes de mésophylle mis en culture dans le milieu B5 de Gamborg se divisent 2 à 3 fois. Des essais de fusion réalisés avec des faibles densités cellulaires entre protoplastes de mésophylle et protoplastes de nodule ont, donné des taux de fusion de l'ordre de 2,43%.
Summary
With a view to studyin vitro the processes of nodulation and plant-bacteria symbiosis, preparation of mesophyll protoplasts and root nodule protoplasts have been realized fromPhaseolus vulgaris (Constant var.).
For every preparation the process of experimentation and optimum concentrations of cellulase and pectinase diluted in mannitol 0,6M have been defined.
After a 15 days' period, 10 to 15% of mesophyll protoplasts divided twice or even three times in B5 Gamborg's medium. Although we used low rate cell concentrations, root nodule protoplasts and mesophyll protoplasts have been able to fuse in the proportion of 2,43%.
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Eb, P., Bacle, B. & Tailliez, R. Etude de la symbiose Rhizobium-legumineuse: Préparation de cellules isolées pour l'étudein vitro . Plant Soil 88, 223–230 (1985). https://doi.org/10.1007/BF02182448
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DOI: https://doi.org/10.1007/BF02182448