Plant and Soil

, Volume 89, Issue 1–3, pp 3–14 | Cite as

Adaptation to salinity at the plant cell level

  • H. R. Lerner
Mechanisms of Salt Tolerance in Algae and Terrestrial Plants


Various mechanisms of adaptation of plant cells to salinity are reviewed: (1) protection of enzymes and maintenance of turgor by organic solutes; (2) prevention of ion toxicity by compartmentation; and (3) energization of solute transport by the proton pump. All these mechanisms seem to play a role in adaptation. The particular advantages of using salt-adapted cells in suspension culture to identify mechanisms of adaptation are pointed out.

Key words

Adaptation to stress Salinity Suspension culture of higher plant cells 





dehydroxyacetone kinase


difference of potential across a membrane

Δ pH

difference of pH across a membrane


sodium dodecyl sulfate


osmotic pressure


abscisic acid and FCCP, p-trifluoromethoxyphenylhydrazone


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  1. 1.
    Chaleff R S 1981 Genetics of Higher Plants. Applications of Cell Culture, Cambridge University Press, London.Google Scholar
  2. 2.
    Citri N 1973 Conformational adaptability in enzymes.In Advances in Enzymology, vol. 37, Ed. A Meister. pp 397–648. Interscience Pub., New York.Google Scholar
  3. 3.
    Critchley C 1983 Further studies on the role of chloride in photosynthetic O2 evolution in higher plants. Biochim. Biophys. Acta 724, 1–5.Google Scholar
  4. 4.
    Dure III L, Galav G, Chlan C and Pyle J 1983 Developmentally regulated gene sets in cotton embryogenesis.In Plant Molecular Biology: Proceedings of the UCLA Symposia on Molecular and Cellular Biology, new series, vol. 12. Ed. R B Goldberg. pp 331–342. Alan R. Liss Inc., New York.Google Scholar
  5. 5.
    Flowers T J, Troke P F and Yeo A R 1977 The mechanism of salt tolerance in halophytes. Annu. Rev. Plant Physiol. 28, 89–121.CrossRefGoogle Scholar
  6. 6.
    Göring H 1981 Plant responses to effects of adverse physical and chemical environmental factors.In Genetic Responses and Plant Breeding for Resistance to Diseases, Pests and Abiotic Environmental Conditions. Eds. D D Brezhnev and V F Dorofeef. pp 152–164. Vavilov All-Union Scientific Research Institute of Plant Industry, Leningrad.Google Scholar
  7. 7.
    Göring H and Bui Huy Thien 1979 Influence of nutrient deficiency on proline accumulation in the cytoplasm ofZea mays L. seedlings. Biochem. Physiol. Pflanzen 174, 9–16.Google Scholar
  8. 8.
    Greenway H and Munns R 1980 Mechanism of salt tolerance in nonhalophytes. Annu. Rev. Plant Physiol. 31, 149–190.CrossRefGoogle Scholar
  9. 9.
    Hasegawa, P M, Bressan R A and Handa A K 1980 Growth characteristics of NaCl-selected and nonselected cells ofNicotiana tabacum L. Plant Cell Physiol. 21, 1347–1355.Google Scholar
  10. 10.
    Jeschke W D, Stelter W, Reising B and Behl R 1983 Vacuolar Na/K exchange, its occurrence in root cells ofHordeum, Atriplex andZea and its significance for K/Na discrimination in roots. J. Exp. Bot. 34, 964–979.Google Scholar
  11. 11.
    Kaempfer R 1984 Regulation of eukaryotic translation.In Comprehensive Virology, vol. 19. Eds. H Fraenkel-Conrat and R R Wagner. Plenum, New York (In press).Google Scholar
  12. 12.
    Kramer D 1983 The possible role of transfer cells in the adaptation of plants to salinity. Physiol. Plant. 58, 549–555.Google Scholar
  13. 13.
    Lerner H R and Avron M 1977 Dihydroxyacetone kinase activity inDunaliella parva. Plant Physiol. 59, 15–17.Google Scholar
  14. 14.
    Lerner H R, Reinhold L, Guy R, Braun Y, Hasidim M and Poljakoff-Mayber A 1983 Salt activation and inhibition of membrane ATPase from roots of the halophyteAtriplex nummularia. Plant Cell Environ. 6, 501–506.Google Scholar
  15. 15.
    Lerner H R and Reuveni M 1982 Induction of pore formation selectively in the plasmalemma of plant cells by poly-l-lysine treatment: a method for the direct measurement of cytosol solutes in plant cells.In Plasmalemma and Tonoplast: Their Functions in the Plant Cell. Eds. D Marmé, E Marrè and R Hertel. pp 49–52. Elsevier Biomedical Press, Amsterdam.Google Scholar
  16. 16.
    Lerner H R, Sussman I and Avron M 1980 Characterization and partial purification of dihydroxyacetone kinase inDunaliella salina. Biochim. Biophys. Acta 615, 1–9.PubMedGoogle Scholar
  17. 17.
    Marrè E 1979 Fusicoccin: a tool in plant physiology. Annu. Rev. Plant Physiol. 30, 273–288.CrossRefGoogle Scholar
  18. 18.
    Munns R, Greenway H and Kirst G D 1983 Halotolerant eukaryotes.In Encyclopedia of Plant Physiology, New Series, vol 12C. Eds. O L Lange, P S Nobel, C B Osmond and H Ziegler. pp 59–135. Springer-Verlag, Berlin.Google Scholar
  19. 19.
    Nash D, Paleg L G and Wiskich J T 1982 Effect of proline, betaine and some other solutes on the heat stability of mitochondrial enzymes. Aust. J. Plant Physiol. 9, 47–57.Google Scholar
  20. 20.
    Nuss D L and Koch G 1976 Variation in the relative synthesis of immunoglobulin G and non-immunoglobulin G proteins in cultured MPC11 cells with changes in the overall rate of polypeptide chain initiation and elongation. J. Mol. Biol. 102, 601–612.CrossRefPubMedGoogle Scholar
  21. 21.
    Paleg L G, Douglas T J, van Dall A and Keech D B 1981 Proline, betaine and other organic solutes protect enzymes against heat inactivation. Aust. J. Plant Physiol. 8, 107–114.Google Scholar
  22. 22.
    Pitman M G and Luttge V 1983 The ionic environment and plant ionic relations.In Encyclopedia of Plant Physiology, New Series, vol 12C. Eds. O L Lange, P S Nobel, C B Osmond and H Ziegler. pp 5–34. Springer-Verlag, Berlin.Google Scholar
  23. 23.
    Singh N K, Handa A K, Hasegawa P M and Bressan R A 1984 Expression of salt-tolerance-specific proteins in cultured tobacco cells. 35th Annual Meeting of the Tissue Culture Association, Houston, Texas. June 3–7, 1984.Google Scholar
  24. 24.
    Singh N K, La Rosa P C, Bressan R A, Hasegawa P M and Handa A K 1984 Protein synthesis in response to NaCl adaptation and abscisic acid. 1984 UCLA Symposia.Google Scholar
  25. 25.
    Stewart C R and Hanson A D 1980 Proline accumulation as a response to water stress.In Adaptation of Plants to Water and High Temperature Stress. Eds. N C Turner and P J Kramer. pp 173–189. J. Wiley, New York.Google Scholar
  26. 26.
    Watad A A, Reinhold L and Lerner H R 1983 Comparison between a stable NaCl-selectedNicotiana cell line and the wild type. Plant Physiol. 73, 624–629.Google Scholar
  27. 27.
    Wyn Jones R G and Gorham J 1983 Osmoregulation.In Encyclopedia of Plant Physiology, New Series, vol 12C. Eds. O L Lange, P S Nobel, C B Osmond and H Ziegler. pp 35–58. Springer-Verlag, Berlin.Google Scholar
  28. 28.
    Wyn Jones R G and Pollard A 1983 Proteins, enzymes and inorganic ions.In Encyclopedia of Plant Physiology, New Series, vol 15. Eds. A Lauchli and R L Bieleski. pp 528–562. Springer-Verlag, Berlin.Google Scholar
  29. 29.
    Yeo A R 1983 Salinity resistance: physiologies and prices. Physiol. Plant. 58, 214–222.Google Scholar

Copyright information

© Martinus Nijhoff Publishers 1985

Authors and Affiliations

  • H. R. Lerner
    • 1
  1. 1.Department of BotanyThe Hebrew University of JerusalemJerusalemIsrael

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