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Influence of growth rate on pigment and lipid composition of the microalgaIsochrysis aff.galbana clone T.iso

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Abstract

A continuous culture ofIsochrysis aff.galbana clone T.iso, used to feedPecten maximus larvae at IFREMER (Brest, France), was carried out in a chemostat at its optimum temperature for growth (26 °C). Changes in pigments, lipid class (neutral, glyco- and phospholipids) and degree of fatty acid unsaturation were studied at three different growth rates (0.33, 0.5, 1 d−1). As predicted by chemostat theory, a slow growth rate produced higher cell numbers and higher biomass per unit volume. These cells were low in chlorophylla and carotenoids, but rich in neutral lipids. In contrast, cultures with a fast growth rate yielded lower cell concentrations, buth higher chlorophylla, carotenoid and membrane lipid contents per cell. Changes in polyunsaturated fatty acid distribution were related to differences in algal growth rates. Neutral lipids contained mainly saturated and monounsaturated fatty acids (C18:1ω9) at low growth rates whereas they were enriched in polyunsaturated fatty acids, especially C22:6ω3, at high growth rates. Therefore, it is suggested that the growth rate in continuous cultures be controlled so as to adjust the relative proportions of polyunsaturated fatty acids in lipid classes of the diet meant for larval nutrition.

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References

  • Arao T, Kawaguchi A, Yamada M (1987) Positional distribution of fatty acids in lipids of the marine diatomPhaedactylum tricornutum. Phytochemistry 26: 2576–2578.

    Google Scholar 

  • Berner T, Wyman K, Dubinsky ZK, Falkowski PG (1989) Photoadaptation and the “package” effect inDunaliella tertiolecta (Chlorophyceae). J. Phycol. 25: 70–78.

    Google Scholar 

  • Bligh EG, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can. J. Biochem. Physiol. 37: 911–917.

    PubMed  Google Scholar 

  • Chretiennot-Dinet MJ, Robert R, His E (1986) Utilisation des “algues fourrages” en aquaculture. Ann. Biol. 25: 97–119.

    Google Scholar 

  • Cohen Z, Vonshak A, Richmond A (1987) Fatty acid composition ofSpirulina strains grown under various environmental conditions. Phytochemistry 26: 2255–2258.

    Google Scholar 

  • Cohen Z, Vonshak A, Richmond A (1988) Effect of environmental conditions on fatty acid composition of the red algaPorphyridium crientum: correlation to growth rate. J. Phycol. 24: 328–332.

    Google Scholar 

  • Darley WM, Volcani BE (1969) Role of silicon in diatom metabolism. A silicon requirement for DNA synthesis in the diatomCylindrotheca fusiformis. Exp. Cell Res. 58: 334–342.

    PubMed  Google Scholar 

  • Dunstan GH, Volkman JK, Barret SM, Garland CD (1993) Changes in the lipid composition and maximisation of the polyunsaturated fatty acid content of three microalgae grown in mass culture. J. appl. Phycol. 5: 71–83.

    Google Scholar 

  • Emdadi D, Berland B (1989) Variation in lipid class composition during batch growth ofNannochloropsis salina andPavlova lutheri. Mar. Chem. 26: 215–225.

    Google Scholar 

  • Enright CT, Newkirk GF, Cragie JS, Castell JS (1986) Evaluation of phytoplankton as diet of juvenileOstea edulis L. J. exp. mar. Biol. Ecol. 96: 1–13.

    Google Scholar 

  • Ewart JW, Pruder GD (1981) Comparative growth ofIsochrysis galbana Parke andIsochrysis aff.galbana Clone T.iso at four temperatures and three light intensities. J. world Maricult. Soc. 12: 333–339.

    Google Scholar 

  • Harrison PJ, Thompson PA, Calderwood GS (1990) Effects of nutrients and light limitation on the biochemical composition of phytoplankton. J. appl. Phycol. 2: 45–56.

    Google Scholar 

  • Henderson RJ, Mackinlay EE (1989) Effect of temperature on lipid composition of the marine cryptomonadChroomonas salina. Phytochemistry 28: 2943–2948.

    Google Scholar 

  • Herzig R, Falkowski PG (1989) Nitrogen limitation inIsochrysis galbana (Haptophyceae). I-Photosynthetic energy conversion and growth efficiences. J. Phycol. 25: 462–471.

    Google Scholar 

  • Kanazawa A, Teschima SI, Sakamato M (1985) Effects of dietary lipids, fatty acids, and phospholipids on growth and survival of prawn (Penaeus japonicus). Aquaculture 50: 39–49.

    Google Scholar 

  • Langdon CJ, Waldock MJ (1981) The effect of algal and artificial diets on the growth and fatty acid composition ofCrassostrea gigas spat. J. mar. biol. Assoc. U.K. 61: 431–448.

    Google Scholar 

  • Marty Y, Delaunay F, Moal J, Samain JF (1992) Changes in the fatty acid composition ofPecten maximus (L.) during larval development. J. exp. mar. Biol. Ecol. 163: 221–234.

    Google Scholar 

  • Mortensen H, Borsheim KU, Rainuzzo JR, Knutsen G (1988) Fatty acid composition of the marine diatomChaetoceros gracilis Schüt. Effects of silicate deprivation, temperature and light intensity. J. exp. mar. Biol. Ecol. 122: 173–185.

    Google Scholar 

  • Napolitano G.E, Ackman R.G, Ratnayake WMN (1990) Fatty acid composition of three cultured algal species (Isochrysis galbana, Chaetoceros gracilis andChaetoceros calcitrans) used as foof for bivalve larvae. J. world. aquaculture Society. 21: 122–130.

    Google Scholar 

  • Okuyama H, Morita N, Kogome K (1992) Occurence of octadecapentaenoic acid in lipids of a cold stenothermic alga, prymnesiophyte strain B. J. Phycol. 28: 465–472.

    Google Scholar 

  • Parsons TR, Maita Y, Lalli CM (1984) A Manual of Chemical and Biological Methods for Seawater Analysis. Pergamon Press, New York, 173 pp.

    Google Scholar 

  • Pillsbury KS (1985) The relative food value and biochemical composition of five phytoplankton diets for queen conch,Strombus gigas (Linne) larvae. J. exp. mar. Biol. Ecol. 90: 221–231.

    Google Scholar 

  • Pohl P, Wagner H (1972) Control fatty acid and lipid biosynthesis inEuglena gracilis by ammonium, light and DCMU. Z. Naturforsch. 27: 53–61.

    Google Scholar 

  • Pohl P, Zurheide F (1979) Fatty acids and lipids of marine algae and the control of their biosynthesis by environmental factors. In Hoppe HA, Levring T, Tanaka Y (eds), Marine Pharmaceutical Science. D. Gruyter, New York: 473–523.

    Google Scholar 

  • Renaud SM, Parry DL, Thinh LV, Kuo C, Padovan A, Sammy N (1991) Effect of light intensity on proximate biochemical and fatty acid composition ofIsochrysis sp. andNannochloropsis oculata for use in tropical aquaculture. J. appl. Phycol. 3: 43–53.

    Google Scholar 

  • Roughan PG, Slack CR (1982) Cellular organisation of glycolipid metabolism. Ann. Rev. Plant. Physiol. 33: 97–132.

    Google Scholar 

  • Shifrin NS, Chisholm SW (1980) Phytoplankton lipids: environmental influences on production and possible commercial applications. In Shelef g, Soeder CJ (eds), Algae Biomass. Elsevier/North Holland Biochemical Press Amsterdam: 627–645.

    Google Scholar 

  • Shifrin NS, Chisholm SW (1981) Phytoplankton lipids: interspecific differences and effects of nitrate, silicate and light-dark cycles. J. Phycol. 17: 374–384.

    Google Scholar 

  • Smith LA, Norman HA, Cho SH, Thompson GA, Jr (1985) Isolation and quantitative analysis of phosphatidylglycerol and glycolipid molecular species using revered phase HPLC with flame ionisation detector. J. Chromatogr. 349: 291–299.

    Google Scholar 

  • Suen Y, Hubbard JS, Holzer G, Tornabene TG (1987) Total lipid production of the green algaNannochloropsis sp. QII under different nitrogen regimes. J. Phycol. 23: 289–296.

    Google Scholar 

  • Sukenik A, Carmely Y, Berner T (1989) Regulation of fatty acid composition by irradiance level in the eustigmatophyteNannochloropsis sp. J. Phycol. 25: 686–692.

    Google Scholar 

  • Sukenik A, Whanon R (1991) Biochemical quality of marine unicellular algae with special emphasis on lipid composition. I.Isochrysis galbana. Aquaculture 97: 61–72.

    Google Scholar 

  • Sukenik A, Zmora O, Carmeli Y (1993) Biochemical quality of marine unicellular algae with special emphasis on lipid composition. II.Nannochloropsis sp. Aquaculture 117: 313–326.

    Google Scholar 

  • Thompson PA, Guo MX, Harrison PJ (1992) Effects of variation in temperature. II On the fatty acid composition of eight species of marine phytoplankton. J. Phycol. 28: 488–497.

    Google Scholar 

  • Thompson PA, Guo MX, Harrison PJ (1993) The influence of irradiance on the biochemical composition of three phytoplankton species and their nutritional value of larvae of the Pacific Oyster (Crassostrea gigas). Mar. Biol. 117: 259–268.

    Google Scholar 

  • Thompson PA, Harrison PJ, Whyte JNC (1990) Influence of irradiance on the fatty acid composition of phytoplankton. J. Phycol. 26: 278–288.

    Google Scholar 

  • Volkman JK, Jeffrey SW, Nichols PD, Rogers GI, Garland CD (1989) Fatty acid and lipid composition of 10 species of microalgae used in mariculture. J. exp. mar. Biol. Ecol. 128: 219–240.

    Google Scholar 

  • Whyte JN (1987) Biochemical composition and energy content of six species of phytoplankton used in mariculture of bivalves. Aquaculture 60: 231–241.

    Google Scholar 

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Saoudi-Helis, L., Dubacq, J.P., Marty, Y. et al. Influence of growth rate on pigment and lipid composition of the microalgaIsochrysis aff.galbana clone T.iso. J Appl Phycol 6, 315–322 (1994). https://doi.org/10.1007/BF02181945

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  • DOI: https://doi.org/10.1007/BF02181945

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