Effect of inoculation ofAzospirillum lipoferum on nitrogen fixation in rhizosphere soil, their association with root, yield and nitrogen uptake by mustard (Brassica juncea)
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A microplot field experiment was conducted in the presence or absence of P and N application to evaluate the influence of the seed inoculation of mustard (cv. Baruna T59) withAzospirillum lipoferum on N2-fixation in rhizosphere, association of the bacteria with the roots and grain yield and N uptake. Inoculation significantly increased the N content in rhizosphere soil particularly at early stage (40 days) of plant growth, which was accompanied by the increased association of the bacteria (A. lipoferum) in rhizosphere soil, root surface washing and surface-sterilized macerated root. A significant increase in grain yield and N uptake was also observed due to inoculation. Application of P particularly at the 20 kg. ha−1 level further enhanced the beneficial effect ofAzospirillum lipoferum inoculation, while N addition markedly reduced such an effect.
Key wordsAzospirillum Mustard Nitrogen fixation Rhizosphere soil Root association Seed inoculation
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- 1.Alexander M 1965 Most probable number method for microbial populations.In Methods of Soil Analysis, Part 2 (C. A. Black, ed) pp. 1467–1472, Am. Soc. Agron. Inc, Madison, Wisconsin, USA.Google Scholar
- 2.Boddey R M and Dobereiner J 1982 Association of Azospirillum and other diazotrophs with tropical Gramineace. Trans. 12th International Cong. Soil Sci., Symposia Paper 1, 28–47.Google Scholar
- 3.Bulow J F W Von and Dobereiner J 1975 Potential for nitrogen fixation in maize genotypes in Brazil. Proc. Nat. Acad. Sci (USA) 72, 2389–2393.Google Scholar
- 5.Jackson M L 1967 Soil Chemical Analysis. Prentice Hall of India Private Limited, New Delhi.Google Scholar
- 6.Lakshmi-Kumari M, Kavimandan S K and Subba Rao N S 1976 Occurrence of nitrogen-fixing Spirillum in roots of sorghum, maize and other plants. Indian J. Exp. Biol. 14, 638–639.Google Scholar
- 9.Rai S N and Gaur A C 1982 Nitrogen fixation byAzospirillum spp. and effect ofAzospirillum lipoferum on the yield and nitrogen uptake of wheat crop. Pl. Soil 69, 233–238.Google Scholar
- 10.Sanoria C L, Singh K L, Ramamurthy K and Maurya B R 1982 Field trials withAzospirillum brasilense in an Indo-Gangetic Alluvial. J. Indian Soc. Soil Sci. 30, 208–209.Google Scholar
- 11.Subba Rao N S, Tilak K V B R, Singh C S and Lakshmi-Kumari M 1979 Response of a few economic species of graminaceous plants to inoculation withAzospirillum brasilense. Curr. Sci. 48, 133–134.Google Scholar
- 12.Subba Rao N S 1979 Crop response to microbial inoculation.In Recent Advances in Biological Nitrogen Fixation (ed. N S Subba Rao), pp 406–420. Oxford and IBH Publishing Co.Google Scholar
- 13.Tarrand J J, Kreig N R and Dobereiner J 1978 A taxonomic study of theSpirillum lipoferum group, with description of a new genus, Azospirillum gen. nov. and two speciesAzospirillum lipoferum (Beijerinck) comb. nov. andAzospirillum brasilense Sp. nov. Can. J. Microbiol 24, 669–980.Google Scholar