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Refractometric determination of sulphate and other anions in plants separated by High-Performance Liquid Chromatography

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Summary

Anion exchange chromatography by High Performance Liquid Chromatography (HPLC) and the refractometric detection of the separated anions proved to be a rapid and sensitive method for the simultaneous determination of sulphate, nitrate, nitrite, chloride and phosphate in plants. Anion content was quantified using sodium selenate as internal standard. Only 20 μl of a ten times diluted deproteinized plant extract was needed for determination.

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Authors and Affiliations

  1. Department of Plant Physiology, University of Groningen, P.O. Box 14, 9750 AA, Haren (Gn), The Netherlands

    Frank M. Maas, Ingrid Hoffmann, Marian J. Van Harmelen & Luit J. De Kok

Authors
  1. Frank M. Maas
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  2. Ingrid Hoffmann
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  3. Marian J. Van Harmelen
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  4. Luit J. De Kok
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Maas, F.M., Hoffmann, I., Van Harmelen, M.J. et al. Refractometric determination of sulphate and other anions in plants separated by High-Performance Liquid Chromatography. Plant Soil 91, 129–132 (1986). https://doi.org/10.1007/BF02181825

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  • DOI: https://doi.org/10.1007/BF02181825

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