Abstract
Serosal preincubation of frog skin with tetradecanoyl phorbol acetate, TPA, an activator of protein kinase C, inhibits the hydrosmotic response elicited by vasopressin (AVP) but not that induced by 8br-cAMP. This proves that serosal TPA primarily influences a pre-cAMP step. The TPA-induced inhibition of AVP response appears to be related to TPA-induced prostaglandin synthesis. The pretreatment with naproxen, in fact, prevents the inhibition induced by serosal TPA on the AVP response. On the contrary, mucosal TPA produces a more marked inhibition of the response to AVP and significantly diminishes the water flow induced by 8br-cAMP; this suggests that mucosal TPA interferes mainly with a post-cAMP step. Furthermore, naproxen is unable to completely prevent the inhibition induced by mucosal TPA on AVP response thus indicating that mucosal TPA may also activate a prostaglandin-independent mechanism able to inhibit one of the last steps of the hydrosmotic response to AVP.
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References
Bourguet J, Jard S (1964) Un dispositif automatique de mesure et d'enregistrement du flux net d'eau a travers la peau et la vessie des amphibiens. Biochim Biophys Acta 88: 442–444.
Kelleher D J, Pessin J E, Ruoho A E, Johnson G L (1984) Phorbol ester induces desensitization of adenylate cyclase and phosphorylation of the Badrenergic receptors in turkey erytrocytes. Proc. Nat. Acad. Sci. 81: 4316–4320.
Masur S K, Sapirstein V, Rivero D (1985) Phorbol myristate acetate induces endocytosis as well as exocytosis and hydrosmosis in toad urinary bladder. Biochim. Biophys Acta 821: 286–296.
Nishizuka Y (1984) The role of protein kinase C in cell surface signal transduction and tumor promotion. Nature 308: 693–697.
Ohuchi K, Levine L (1978) Stimulation of prostaglandin synthesis by tumor-promoting phorbol 12, 13 diesters in canine kidney (MCDK) cells. J Biol Chem. 253: 4783–4790.
Orloff J, Zusmann R (1978) Role of prostaglandin E (PGE) on water flow in the urinary bladder of the toad and mammalian kidney. J Membrane Biol Special issue 40:293–304.
Schlondorff D, Satriano J A (1985) Interaction of vasopressin, cAMP and prostaglandins in toad urinary bladder. Am J Physiol 248: F454-F458.
Schlondorff D, Levine S D (1985) Inhibition of vasopressin-stimulated water flow in toad bladder by Phorbol Myristate acetate Dioctanoylglycerol and RHC-80267. J Clin Invest 76: 1071–1078.
Seamon K B, Daly J W (1983) Forskolin, cyclic AMP and cellular physiology. Trend Pharmacol Sci 4: 120–123.
Sibley D R, Nambi P, Peters J P, Lefkowitz R J (1984) Phorbol diesters promote B-adrenergic receptors phosphorylation and adenylate cyclase desensitization in duck erytrocytes. Biochem biophys Res Commun 121: 973–979.
Streb H, Irvine R F, Berridge M J, Schultz I (1983) Release of Ca++ from a non-mitochondrial store of pancreatic acinar cells by inositol 1, 4, 5-triphosphate. Nature 306: 67–69.
Svelto M, Casavola V (1985) Hydrosmotic response to vasopressin in frog skin. Control by endogenous factors. Biol Cell 55: 199–206.
Thomas A P, Marks J S, Coll K E, Williamson J B (1983) Quantitation and early kinetics of inositol lipid changes induced by vasopressin in isolated and cultured hepatocytes. J Biol Chem 258: 5716–5725.
Yorio T, Quist E, Masaracchia R (1985) Calcium phospholipid-dependent protein kinase and its relationship to antidiuretic hormone in toad urinary bladder epithelium. Biochem. Biophys Res Commun 133: 713–723.
Zusman R M, Keiser HR, Handler J S (1978) Effect of adrenal steroids on vasopressin stimulated PGE synthesis and water flow. Am J Physiol 234: F532-F540.
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Casavola, V., Iacovelli, L. & Svelto, M. Phorbol ester effect on the hydrosmotic response to vasopressin in frog skin. Pflugers Arch. 408, 318–320 (1987). https://doi.org/10.1007/BF02181476
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DOI: https://doi.org/10.1007/BF02181476